TY  - JOUR
A1  - Fandrich, Artur
A1  - Buller, Jens
A1  - Schäfer, Daniel
A1  - Wischerhoff, Erik
A1  - Laschewsky, André
A1  - Lisdat, Fred
T1  - Electrochemical characterization of a responsive macromolecular interface on gold
JF  - Physica status solidi : A, Applications and materials science
N2  - This study reports on the investigation of a thermoresponsive polymer as a thin film on electrodes and the influence of coupling a peptide and an antibody to the film. The utilized polymer from the class of poly(oligoethylene glycol)-methacrylate polymers (poly(OEGMA)) with carboxy functions containing side chains was synthesized and properly characterized in aqueous solutions. The dependence of the cloud point on the pH of the surrounding media is discussed. The responsive polymer was immobilized on gold electrodes as shown by electrochemical, quartz crystal microbalance (QCM), and atomic force microscopy (AFM) techniques. The temperature dependent behavior of the polymer covalently grafted to gold substrates is investigated using cyclic voltammetry (CV) in ferro-/ferricyanide solution. Significant changes in the slope of the temperature-dependence of the voltammetric peak current and the peak separation values clearly indicate the thermally induced conformational change on the surface. Finally, a biorecognition reaction between a short FLAG peptide (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C) covalently immobilized on the polymer interface and the corresponding IgG antibody was performed. The study shows that the responsiveness of the electrode is retained after peptide coupling and antibody binding, although the response is diminished.
KW  - biorecognition reactions
KW  - cyclic voltammetry
KW  - electrodes
KW  - gold
KW  - interfaces
KW  - responsive polymers
Y1  - 2015
U6  - https://doi.org/10.1002/pssa.201431698
SN  - 1862-6300
SN  - 1862-6319
VL  - 212
IS  - 6
SP  - 1359
EP  - 1367
PB  - Wiley-VCH
CY  - Weinheim
ER  -