TY  - JOUR
A1  - Haralampiev, Ivan
A1  - Mertens, Monique
A1  - Schwarzer, Roland
A1  - Herrmann, Andreas
A1  - Volkmer, Rudolf
A1  - Wessig, Pablo
A1  - Mueller, Peter
T1  - Recruitment of SH-Containing peptides to lipid and biological membranes through the use of a palmitic acid functionalized with a Maleimide Group
JF  - Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition
N2  - This study presents a novel and easily applicable approach to recruit sulfhydryl-containing biomolecules to membranes by using a palmitic acid which is functionalized with a maleimide group. Notably, this strategy can also be employed with preformed (biological) membranes. The applicability of the assay is demonstrated by characterizing the binding of a Rhodamine-labeled peptide to lipid and cellular membranes using methods of fluorescence spectroscopy, lifetime measurement, and microscopy. Our approach offers new possibilities for preparing biologically active liposomes and manipulating living cells.
KW  - liposomes
KW  - maleimide
KW  - membranes
KW  - palmitic acid
KW  - palmitoylation
KW  - peptides
Y1  - 2015
U6  - https://doi.org/10.1002/anie.201408089
SN  - 1433-7851
SN  - 1521-3773
VL  - 54
IS  - 1
SP  - 323
EP  - 326
PB  - Wiley-VCH
CY  - Weinheim
ER  -