TY  - JOUR
A1  - Lau, Skadi
A1  - Maier, Anna
A1  - Braune, Steffen
A1  - Gossen, Manfred
A1  - Lendlein, Andreas
T1  - Effect of endothelial culture medium composition on platelet responses to polymeric biomaterials
JF  - International journal of molecular sciences
N2  - Near-physiological in vitro thrombogenicity test systems for the evaluation of blood-contacting endothelialized biomaterials requires co-cultivation with platelets (PLT). However, the addition of PLT has led to unphysiological endothelial cell (EC) detachment in such in vitro systems. A possible cause for this phenomenon may be PLT activation triggered by the applied endothelial cell medium, which typically consists of basal medium (BM) and nine different supplements. To verify this hypothesis, the influence of BM and its supplements was systematically analyzed regarding PLT responses. For this, human platelet rich plasma (PRP) was mixed with BM, BM containing one of nine supplements, or with BM containing all supplements together. PLT adherence analysis was carried out in six-channel slides with plasma-treated cyclic olefin copolymer (COC) and poly(tetrafluoro ethylene) (PTFE, as a positive control) substrates as part of the six-channel slides in the absence of EC and under static conditions. PLT activation and aggregation were analyzed using light transmission aggregometry and flow cytometry (CD62P). Medium supplements had no effect on PLT activation and aggregation. In contrast, supplements differentially affected PLT adherence, however, in a polymer- and donor-dependent manner. Thus, the use of standard endothelial growth medium (BM + all supplements) maintains functionality of PLT under EC compatible conditions without masking the differences of PLT adherence on different polymeric substrates. These findings are important prerequisites for the establishment of a near-physiological in vitro thrombogenicity test system assessing polymer-based cardiovascular implant materials in contact with EC and PLT.
KW  - cyclic olefin copolymer
KW  - poly(tetrafluoroethylene)
KW  - endothelial cells
KW  - platelets
KW  - in vitro thrombogenicity testing
Y1  - 2021
U6  - https://doi.org/10.3390/ijms22137006
SN  - 1422-0067
SN  - 1661-6596
VL  - 22
IS  - 13
PB  - Molecular Diversity Preservation International
CY  - Basel
ER  -