TY  - JOUR
A1  - Liu, Songqin
A1  - Wollenberger, Ursula
A1  - Katterle, Martin
A1  - Scheller, Frieder W.
T1  - Ferroceneboronic acid-based amperometric biosensor for glycated hemoglobin
N2  - An amperometric biosensor for the determination of glycated hemoglobin in human whole blood is proposed. The principle is based on the electrochemical measurement of ferroceneboronic acid (FcBA) that has been specifically bound to the glycated N-terminus. Hemoglobin is immobilized on a zirconium dioxide nanoparticle modified pyrolytic graphite electrode (PGE) in the presence of didodecyldimethylammonium bromide (DDAB). The incubation of this sensor in FcBA solution leads to the formation of an FcBA-modified surface due to the affinity interaction between boronate and the glycated sites of the hemoglobin. The binding of FcBA results in well-defined redox peaks with an E-0' of 0.299 V versus Ag/AgCl (1 M KCl). The square wave voltammetric response of the bound FcBA reflects the amount of glycated hemoglobin at the surface. This signal increases linearily with the degree of glycated hemoglobin from 6.8 to 14.0% of total immobilized hemoglobin. The scheme was applied to the determination of the fraction of glycated hemoglobin in whole blood samples.
Y1  - 2006
UR  - http://www.sciencedirect.com/science/journal/09254005
U6  - https://doi.org/10.1016/j.snb.2005.07.011
SN  - 0925-4005
ER  -