TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Makower, Alexander
A1  - Ghindilis, A. L.
A1  - Bier, Frank Fabian
A1  - Ehrentreich-Förster, Eva
A1  - Wollenberger, Ursula
A1  - Bauer, Christian G.
A1  - Micheel, Burkhard
A1  - Pfeiffer, Dorothea
A1  - Szeponik, Jan
A1  - Michael, N.
A1  - Kaden, H.
T1  - Enzyme sensors for subnanomolar concentrations
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Krylov, Andrey. V.
A1  - Adamzig, H.
A1  - Walter, A. D.
A1  - Loechel, B.
A1  - Kurth, E.
A1  - Pulz, O.
A1  - Szeponik, Jan
A1  - Wegerich, Franziska
A1  - Lisdat, Fred
T1  - Parallel generation and detection of superoxide and hydrogen peroxide in a fluidic chip
JF  - Sensors and actuators : B, Chemical
N2  - A fluidic chip system was developed, which combines a stable generation of superoxide radicals and hydrogen peroxide with their sensorial detection. The generation of both reactive oxygen species was achieved by immobilization of xanthine oxidase on controlled pore glass in a reaction chamber. Antioxidants can be introduced into the fluidic chip system by means of mixing chamber. The detection of both species is based on the amperometric principle using a biosensor chip with two working electrodes. As sensing protein for both electrodes cytochrome c was used. The novel system was designed for the quantification of the antioxidant efficiency of different potential scavengers of the respective reactive species in an aqueous medium. Several model antioxidants such as ascorbic acid or catalase have been tested under flow conditions.
KW  - biosensor
KW  - cytochrome c
KW  - flow system
KW  - reactive oxygen species
KW  - antioxidant
Y1  - 2006
U6  - https://doi.org/10.1016/j.snb.2005.11.062
SN  - 0925-4005
VL  - 119
IS  - 1
SP  - 118
EP  - 126
PB  - Elsevier
CY  - Lausanne
ER  - 
TY  - JOUR
A1  - Pinyou, Piyanut
A1  - Ruff, Adrian
A1  - Poeller, Sascha
A1  - Barwe, Stefan
A1  - Nebel, Michaela
A1  - Alburquerque, Natalia Guerrero
A1  - Wischerhoff, Erik
A1  - Laschewsky, Andre
A1  - Schmaderer, Sebastian
A1  - Szeponik, Jan
A1  - Plumere, Nicolas
A1  - Schuhmann, Wolfgang
T1  - Thermoresponsive amperometric glucose biosensor
JF  - Biointerphases
N2  - The authors report on the fabrication of a thermoresponsive biosensor for the amperometric detection of glucose. Screen printed electrodes with heatable gold working electrodes were modified by a thermoresponsive statistical copolymer [polymer I: poly(omega-ethoxytriethylenglycol methacrylate-omega-3-(N,N-dimethyl-N-2-methacryloyloxyethyl ammonio) propanesulfonate-co-omega-butoxydiethylenglycol methacrylate-co-2-(4-benzoyl-phenoxy)ethyl methacrylate)] with a lower critical solution temperature of around 28 degrees C in aqueous solution via electrochemically induced codeposition with a pH-responsive redox-polymer [polymer II: poly(glycidyl methacrylate-co-allyl methacrylate-co-poly(ethylene glycol) methacrylate-co-butyl acrylate-co-2-(dimethylamino) ethyl methacrylate)-[Os(bpy)(2)(4-(((2-(2-(2-aminoethoxy) ethoxy) ethyl) amino) methyl)-N,N-dimethylpicolinamide)](2+)] and pyrroloquinoline quinone-soluble glucose dehydrogenase acting as biological recognition element. Polymer II bears covalently bound Os-complexes that act as redox mediators for shuttling electrons between the enzyme and the electrode surface. Polymer I acts as a temperature triggered immobilization matrix. Probing the catalytic current as a function of the working electrode temperature shows that the activity of the biosensor is dramatically reduced above the phase transition temperature of polymer I. Thus, the local modulation of the temperature at the interphase between the electrode and the bioactive layer allows switching the biosensor from an on-to an off-state without heating of the surrounding analyte solution. (C) 2015 American Vacuum Society.
Y1  - 2016
U6  - https://doi.org/10.1116/1.4938382
SN  - 1934-8630
SN  - 1559-4106
VL  - 11
PB  - American Institute of Physics
CY  - Melville
ER  - 
TY  - JOUR
A1  - Szeponik, Jan
A1  - Möller, B.
A1  - Pfeiffer, Dorothea
A1  - Lisdat, Fred
A1  - Wollenberger, Ursula
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
T1  - Ultrasensitive bienzyme sensor for adrenaline
Y1  - 1997
ER  -