TY - JOUR A1 - Sass, Enrico T1 - Academic entrepreneurship BT - Why do university scientists play the entrepreneurship game? JF - Erkenntnisse zum Wissens- und Technologietransfer N2 - Research on entrepreneurial motivation of university scientists is often determined by quantitative methods without taking into account context-related influences. According to different studies, entrepreneurial scientists found a spin-off company due to motives like independency, market opportunity, money or risk of unemployment (short-term contracts). To give a comprehensive explanation, it is important to use a qualitative research view that considers academic rank, norms and values of university scientists. The author spoke with 35 natural scientists and asked professors and research fellows for their entrepreneurial motivation. The results of this study are used to develop a typology of entrepreneurial and non-entrepreneurial scientists within German universities. This paper presents the key findings of the study (Sass 2011). KW - entrepreneurial scientists KW - academic entrepreneurship KW - entrepreneurial motivation KW - entrepreneurial types KW - university spin-offs Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-66873 ER - TY - JOUR A1 - Sass, Enrico T1 - Neugierde und Wettkampfsport BT - die Gründungsmotivation von Professoren JF - Erkenntnisse zum Wissens- und Technologietransfer N2 - Ausgründungen aus der Wissenschaft (spin-offs) gehören zu den anspruchsvollsten Instrumenten des Wissens- und Technologietransfers. Die Initiatoren erfolgreicher Gründungsvorhaben sind oftmals engagierte Hochschullehrer, die nicht nur Anerkennung in der Scientific Community suchen, sondern ihre Forschungsergebnisse ebenso in anwendungsorientierte Produkte und Dienstleistungen überführen. Was treibt diese Wissenschaftler an? In welchem Zusammenhang steht die Gründungsmotivation mit der ursprünglichen wissenschaftlichen Motivation? Ist das Initiieren einer Ausgründung mehr als das Lösen eines herausfordernden Rätsels? Der vorliegende Artikel gibt eine Antwort auf diese Fragen und gewährt einen Einblick in die Gründungsmotivation von Hochschulprofessoren aus den Naturwissenschaften. Mit Hilfe einer qualitativen Untersuchung werden verschiedene Gründertypen gebildet. KW - Gründungsmotivation KW - Gründungsmotive KW - Spin-off-Gründer KW - academic entrepreneurship Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-66159 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Stefanakis, Nikolaos A1 - Abel, Markus A1 - Bergner, Andre T1 - Sound Synthesis Based on Ordinary Differential Equations JF - Computer music journal N2 - Ordinary differential equations (ODEs) have been studied for centuries as a means to model complex dynamical processes from the real world. Nevertheless, their application to sound synthesis has not yet been fully exploited. In this article we present a systematic approach to sound synthesis based on first-order complex and real ODEs. Using simple time-dependent and nonlinear terms, we illustrate the mapping between ODE coefficients and physically meaningful control parameters such as pitch, pitch bend, decay rate, and attack time. We reveal the connection between nonlinear coupling terms and frequency modulation, and we discuss the implications of this scheme in connection with nonlinear synthesis. The ability to excite a first-order complex ODE with an external input signal is also examined; stochastic or impulsive signals that are physically or synthetically produced can be presented as input to the system, offering additional synthesis possibilities, such as those found in excitation/filter synthesis and filter-based modal synthesis. Y1 - 2015 U6 - https://doi.org/10.1162/COMJ_a_00314 SN - 0148-9267 SN - 1531-5169 VL - 39 IS - 3 SP - 46 EP - 58 PB - MIT Press CY - Cambridge ER - TY - JOUR A1 - Tscheuschner, Georg A1 - Kaiser, Melanie N. A1 - Lisec, Jan A1 - Beslic, Denis A1 - Muth, Thilo A1 - Krüger, Maren A1 - Mages, Hans Werner A1 - Dorner, Brigitte G. A1 - Knospe, Julia A1 - Schenk, Jörg A. A1 - Sellrie, Frank A1 - Weller, Michael G. T1 - MALDI-TOF-MS-based identification of monoclonal murine Anti-SARS-CoV-2 antibodies within one hour JF - Antibodies N2 - During the SARS-CoV-2 pandemic, many virus-binding monoclonal antibodies have been developed for clinical and diagnostic purposes. This underlines the importance of antibodies as universal bioanalytical reagents. However, little attention is given to the reproducibility crisis that scientific studies are still facing to date. In a recent study, not even half of all research antibodies mentioned in publications could be identified at all. This should spark more efforts in the search for practical solutions for the traceability of antibodies. For this purpose, we used 35 monoclonal antibodies against SARS-CoV-2 to demonstrate how sequence-independent antibody identification can be achieved by simple means applied to the protein. First, we examined the intact and light chain masses of the antibodies relative to the reference material NIST-mAb 8671. Already half of the antibodies could be identified based solely on these two parameters. In addition, we developed two complementary peptide mass fingerprinting methods with MALDI-TOF-MS that can be performed in 60 min and had a combined sequence coverage of over 80%. One method is based on the partial acidic hydrolysis of the protein by 5 mM of sulfuric acid at 99 degrees C. Furthermore, we established a fast way for a tryptic digest without an alkylation step. We were able to show that the distinction of clones is possible simply by a brief visual comparison of the mass spectra. In this work, two clones originating from the same immunization gave the same fingerprints. Later, a hybridoma sequencing confirmed the sequence identity of these sister clones. In order to automate the spectral comparison for larger libraries of antibodies, we developed the online software ABID 2.0. This open-source software determines the number of matching peptides in the fingerprint spectra. We propose that publications and other documents critically relying on monoclonal antibodies with unknown amino acid sequences should include at least one antibody fingerprint. By fingerprinting an antibody in question, its identity can be confirmed by comparison with a library spectrum at any time and context. KW - SARS-CoV-2 antibody KW - reproducibility crisis KW - peptide mass KW - fingerprinting KW - monoclonal antibody KW - traceability KW - identity KW - antibody KW - identification KW - antibody light chain KW - MALDI-TOF-MS Y1 - 2022 U6 - https://doi.org/10.3390/antib11020027 SN - 2073-4468 VL - 11 IS - 2 PB - MDPI CY - Basel ER - TY - JOUR A1 - Zurnic, Irena A1 - Hütter, Sylvia A1 - Rzeha, Ute A1 - Stanke, Nicole A1 - Reh, Juliane A1 - Müllers, Erik A1 - Hamann, Martin V. A1 - Kern, Tobias A1 - Gerresheim, Gesche K. A1 - Lindel, Fabian A1 - Serrao, Erik A1 - Lesbats, Paul A1 - Engelman, Alan N. A1 - Cherepanov, Peter A1 - Lindemann, Dirk T1 - Interactions of Prototype Foamy Virus Capsids with Host Cell Polo-Like Kinases Are Important for Efficient Viral DNA Integration JF - PLoS Pathogens N2 - Unlike for other retroviruses, only a few host cell factors that aid the replication of foamy viruses (FVs) via interaction with viral structural components are known. Using a yeast-two-hybrid (Y2H) screen with prototype FV (PFV) Gag protein as bait we identified human polo-like kinase 2 (hPLK2), a member of cell cycle regulatory kinases, as a new interactor of PFV capsids. Further Y2H studies confirmed interaction of PFV Gag with several PLKs of both human and rat origin. A consensus Ser-Thr/Ser-Pro (S-T/S-P) motif in Gag, which is conserved among primate FVs and phosphorylated in PFV virions, was essential for recognition by PLKs. In the case of rat PLK2, functional kinase and polo-box domains were required for interaction with PFV Gag. Fluorescently-tagged PFV Gag, through its chromatin tethering function, selectively relocalized ectopically expressed eGFP-tagged PLK proteins to mitotic chromosomes in a Gag STP motif-dependent manner, confirming a specific and dominant nature of the Gag-PLK interaction in mammalian cells. The functional relevance of the Gag-PLK interaction was examined in the context of replication-competent FVs and single-round PFV vectors. Although STP motif mutated viruses displayed wild type (wt) particle release, RNA packaging and intra-particle reverse transcription, their replication capacity was decreased 3-fold in single-cycle infections, and up to 20-fold in spreading infections over an extended time period. Strikingly similar defects were observed when cells infected with single-round wt Gag PFV vectors were treated with a pan PLK inhibitor. Analysis of entry kinetics of the mutant viruses indicated a post-fusion defect resulting in delayed and reduced integration, which was accompanied with an enhanced preference to integrate into heterochromatin. We conclude that interaction between PFV Gag and cellular PLK proteins is important for early replication steps of PFV within host cells. Y1 - 2016 U6 - https://doi.org/10.1371/journal.ppat.1005860 SN - 1553-7366 SN - 1553-7374 VL - 12 PB - PLoS CY - San Fransisco ER - TY - BOOK T1 - Science goes market : ein Programm zur Entwicklung von Managementqualifikationen und Karrierechancen für Wissenschaftler Y1 - 2009 UR - http://www.epe.ceip.de/files/20090916_epe_brosch__re_nachdruck_2009_komprimiert.pdf CY - Potsdam ER -