TY  - JOUR
A1  - Bornhorst, Julia
A1  - Kipp, Anna P.
A1  - Haase, Hajo
A1  - Meyer, Soeren
A1  - Schwerdtle, Tanja
T1  - The crux of inept biomarkers for risks and benefits of trace elements
JF  - Trends in Analytical Chemistry
N2  - Nowadays, the role of trace elements (TE) is of growing interest because dyshomeostasis of selenium (Se), manganese (Mn), zinc (Zn), and copper (Cu) is supposed to be a risk factor for several diseases. Thereby, research focuses on identifying new biomarkers for the TE status to allow for a more reliable description of the individual TE and health status. This review mirrors a lack of well-defined, sensitive, and selective biomarkers and summarizes technical limitations to measure them. Thus, the capacity to assess the relationship between dietary TE intake, homeostasis, and health is restricted, which would otherwise provide the basis to define adequate intake levels of single TE in both healthy and diseased humans. Besides that, our knowledge is even more limited with respect to the real life situation of combined TE intake and putative interactions between single TE.
KW  - Trace elements
KW  - Copper
KW  - Zinc
KW  - Manganese
KW  - Selenium
KW  - Biomarker
KW  - Inductively coupled plasma mass spectrometry
KW  - Hyphenated techniques
KW  - Isotope ratios
Y1  - 2018
U6  - https://doi.org/10.1016/j.trac.2017.11.007
SN  - 0165-9936
SN  - 1879-3142
VL  - 104
SP  - 183
EP  - 190
PB  - Elsevier
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Gubert, Priscila
A1  - Puntel, Bruna
A1  - Lehmen, Tassia
A1  - Fessel, Joshua P.
A1  - Cheng, Pan
A1  - Bornhorst, Julia
A1  - Trindade, Lucas Siqueira
A1  - Avila, Daiana S.
A1  - Aschner, Michael
A1  - Soares, Felix A. A.
T1  - Metabolic effects of manganese in the nematode Caenorhabditis elegans through DAergic pathway and transcription factors activation
JF  - Neurotoxicology : the interdisciplinary journal of effects to toxic substances on the nervous system
N2  - Manganese (Mn) is an essential trace element for physiological functions since it acts as an enzymatic co-factor. Nevertheless, overexposure to Mn has been associated with a pathologic condition called manganism. Furthermore, Mn has been reported to affect lipid metabolism by mechanisms which have yet to be established. Herein, we used the nematode Caenorhabditis elegans to examine Mn’s effects on the dopaminergic (DAergic) system and determine which transcription factors that regulate with lipid metabolism are affected by it. Worms were exposed to Mn for four hours in the presence of bacteria and in a liquid medium (85 mM NaCl). Mn increased fat storage as evidenced both by Oil Red O accumulation and triglyceride levels. In addition, metabolic activity was reduced as a reflection of decreased oxygen consumption caused by Mn. Mn also affected feeding behavior as evidenced by decreased pharyngeal pumping rate. DAergic neurons viability were not altered by Mn, however the dopamine levels were significantly reduced following Mn exposure. Furthermore, the expression of sbp-1 transcription factor and let-363 protein kinase responsible for lipid accumulation control was increased and decreased, respectively, by Mn. Altogether, our data suggest that Mn increases the fat storage in C. elegans, secondary to DAergic system alterations, under the control of SBP-1 and LET-363 proteins.
KW  - Manganese
KW  - Caenorhabditis elegans
KW  - Lipid metabolism
KW  - Dopaminergic system
KW  - Manganism
Y1  - 2018
U6  - https://doi.org/10.1016/j.neuro.2018.04.008
SN  - 0161-813X
SN  - 1872-9711
VL  - 67
SP  - 65
EP  - 72
PB  - Elsevier
CY  - Amsterdam
ER  -