TY  - JOUR
A1  - Allan, Eric
A1  - Bossdorf, Oliver
A1  - Dormann, Carsten F.
A1  - Prati, Daniel
A1  - Gossner, Martin M.
A1  - Tscharntke, Teja
A1  - Blüthgen, Nico
A1  - Bellach, Michaela
A1  - Birkhofer, Klaus
A1  - Boch, Steffen
A1  - Böhm, Stefan
A1  - Börschig, Carmen
A1  - Chatzinotas, Antonis
A1  - Christ, Sabina
A1  - Daniel, Rolf
A1  - Diekötter, Tim
A1  - Fischer, Christiane
A1  - Friedl, Thomas
A1  - Glaser, Karin
A1  - Hallmann, Christine
A1  - Hodac, Ladislav
A1  - Hölzel, Norbert
A1  - Jung, Kirsten
A1  - Klein, Alexandra Maria
A1  - Klaus, Valentin H.
A1  - Kleinebecker, Till
A1  - Krauss, Jochen
A1  - Lange, Markus
A1  - Morris, E. Kathryn
A1  - Müller, Jörg
A1  - Nacke, Heiko
A1  - Pasalic, Esther
A1  - Rillig, Matthias C.
A1  - Rothenwoehrer, Christoph
A1  - Schally, Peter
A1  - Scherber, Christoph
A1  - Schulze, Waltraud X.
A1  - Socher, Stephanie A.
A1  - Steckel, Juliane
A1  - Steffan-Dewenter, Ingolf
A1  - Türke, Manfred
A1  - Weiner, Christiane N.
A1  - Werner, Michael
A1  - Westphal, Catrin
A1  - Wolters, Volkmar
A1  - Wubet, Tesfaye
A1  - Gockel, Sonja
A1  - Gorke, Martin
A1  - Hemp, Andreas
A1  - Renner, Swen C.
A1  - Schöning, Ingo
A1  - Pfeiffer, Simone
A1  - König-Ries, Birgitta
A1  - Buscot, Francois
A1  - Linsenmair, Karl Eduard
A1  - Schulze, Ernst-Detlef
A1  - Weisser, Wolfgang W.
A1  - Fischer, Markus
T1  - Interannual variation in land-use intensity enhances grassland multidiversity
JF  - Proceedings of the National Academy of Sciences of the United States of America
N2  - Although temporal heterogeneity is a well-accepted driver of biodiversity, effects of interannual variation in land-use intensity (LUI) have not been addressed yet. Additionally, responses to land use can differ greatly among different organisms; therefore, overall effects of land-use on total local biodiversity are hardly known. To test for effects of LUI (quantified as the combined intensity of fertilization, grazing, and mowing) and interannual variation in LUI (SD in LUI across time), we introduce a unique measure of whole-ecosystem biodiversity, multidiversity. This synthesizes individual diversity measures across up to 49 taxonomic groups of plants, animals, fungi, and bacteria from 150 grasslands. Multidiversity declined with increasing LUI among grasslands, particularly for rarer species and aboveground organisms, whereas common species and belowground groups were less sensitive. However, a high level of interannual variation in LUI increased overall multidiversity at low LUI and was even more beneficial for rarer species because it slowed the rate at which the multidiversity of rare species declined with increasing LUI. In more intensively managed grasslands, the diversity of rarer species was, on average, 18% of the maximum diversity across all grasslands when LUI was static over time but increased to 31% of the maximum when LUI changed maximally over time. In addition to decreasing overall LUI, we suggest varying LUI across years as a complementary strategy to promote biodiversity conservation.
KW  - biodiversity loss
KW  - agricultural grasslands
KW  - Biodiversity Exploratories
Y1  - 2014
U6  - https://doi.org/10.1073/pnas.1312213111
SN  - 0027-8424
VL  - 111
IS  - 1
SP  - 308
EP  - 313
PB  - National Acad. of Sciences
CY  - Washington
ER  - 
TY  - JOUR
A1  - Allu, Annapurna Devi
A1  - Soja, Aleksandra Maria
A1  - Wu, Anhui
A1  - Szymanski, Jedrzej
A1  - Balazadeh, Salma
T1  - Salt stress and senescence: identification of cross-talk regulatory components
JF  - Journal of experimental botany
N2  - Leaf senescence is an active process with a pivotal impact on plant productivity. It results from extensive signalling cross-talk coordinating environmental factors with intrinsic age-related mechanisms. Although many studies have shown that leaf senescence is affected by a range of external parameters, knowledge about the regulatory systems that govern the interplay between developmental programmes and environmental stress is still vague. Salinity is one of the most important environmental stresses that promote leaf senescence and thus affect crop yield. Improving salt tolerance by avoiding or delaying senescence under stress will therefore play an important role in maintaining high agricultural productivity. Experimental evidence suggests that hydrogen peroxide (H2O2) functions as a common signalling molecule in both developmental and salt-induced leaf senescence. In this study, microarray-based gene expression profiling on Arabidopsis thaliana plants subjected to long-term salinity stress to induce leaf senescence was performed, together with co-expression network analysis for H2O2-responsive genes that are mutually up-regulated by salt induced-and developmental leaf senescence. Promoter analysis of tightly co-expressed genes led to the identification of seven cis-regulatory motifs, three of which were known previously, namely CACGTGT and AAGTCAA, which are associated with reactive oxygen species (ROS)-responsive genes, and CCGCGT, described as a stress-responsive regulatory motif, while the others, namely ACGCGGT, AGCMGNC, GMCACGT, and TCSTYGACG were not characterized previously. These motifs are proposed to be novel elements involved in the H2O2-mediated control of gene expression during salinity stress-triggered and developmental senescence, acting through upstream transcription factors that bind to these sites.
KW  - Arabidopsis
KW  - hydrogen peroxide
KW  - longevity
KW  - reactive oxygen species
KW  - salt stress
KW  - senescence
KW  - signal cross-talk
KW  - transcription factor
Y1  - 2014
U6  - https://doi.org/10.1093/jxb/eru173
SN  - 0022-0957
SN  - 1460-2431
VL  - 65
IS  - 14
SP  - 3993
EP  - 4008
PB  - Oxford Univ. Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Arlt, Olga
A1  - Schwiebs, Anja
A1  - Japtok, Lukasz
A1  - Rueger, Katja
A1  - Katzy, Elisabeth
A1  - Kleuser, Burkhard
A1  - Radeke, Heinfried H.
T1  - Sphingosine-1-Phosphate modulates dendritic cell function: focus on non-migratory effects in vitro and in vivo
JF  - Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry and pharmacology
N2  - Dendritic cells (DCs) are the cutting edge in innate and adaptive immunity. The major functions of these antigen presenting cells are the capture, endosomal processing and presentation of antigens, providing them an exclusive ability to provoke adaptive immune responses and to induce and control tolerance. Immature DCs capture and process antigens, migrate towards secondary lymphoid organs where they present antigens to naive T cells in a well synchronized sequence of procedures referred to as maturation. Indeed, recent research indicated that sphingolipids are modulators of essential steps in DC homeostasis. It has been recognized that sphingolipids not only modulate the development of DC subtypes from precursor cells but also influence functional activities of DCs such as antigen capture, and cytokine profiling. Thus, it is not astonishing that sphingolipids and sphingolipid metabolism play a substantial role in inflammatory diseases that are modulated by DCs. Here we highlight the function of sphingosine 1-phosphate (S1P) on DC homeostasis and the role of SIP and SW metabolism in inflammatory diseases.
KW  - Sphingosine-1-phosphate
KW  - Dendritic cells
KW  - Fingolimod
KW  - IL-12
KW  - Inflammation
Y1  - 2014
U6  - https://doi.org/10.1159/000362982
SN  - 1015-8987
SN  - 1421-9778
VL  - 34
IS  - 1
SP  - 27
EP  - 44
PB  - Karger
CY  - Basel
ER  - 
TY  - THES
A1  - Arnold, Anne
T1  - Modeling photosynthesis and related metabolic processes : from detailed examination to consideration of the metabolic context
T1  - Modellierung von Photosynthese und damit zusammenhängende metabolische Prozesse : von detaillierter Betrachtung hin zur Erörterung im metabolischen Kontext
N2  - Mathematical modeling of biological systems is a powerful tool to systematically investigate the functions of biological processes and their relationship with the environment. To obtain accurate and biologically interpretable predictions, a modeling framework has to be devised whose assumptions best approximate the examined scenario and which copes with the trade-off of complexity of the underlying mathematical description: with attention to detail or high coverage. Correspondingly, the system can be examined in detail on a smaller scale or in a simplified manner on a larger scale. In this thesis, the role of photosynthesis and its related biochemical processes in the context of plant metabolism was dissected by employing modeling approaches ranging from kinetic to stoichiometric models. The Calvin-Benson cycle, as primary pathway of carbon fixation in C3 plants, is the initial step for producing starch and sucrose, necessary for plant growth. Based on an integrative analysis for model ranking applied on the largest compendium of (kinetic) models for the Calvin-Benson cycle, those suitable for development of metabolic engineering strategies were identified. Driven by the question why starch rather than sucrose is the predominant transitory carbon storage in higher plants, the metabolic costs for their synthesis were examined. The incorporation of the maintenance costs for the involved enzymes provided a model-based support for the preference of starch as transitory carbon storage, by only exploiting the stoichiometry of synthesis pathways. Many photosynthetic organisms have to cope with processes which compete with carbon fixation, such as photorespiration whose impact on plant metabolism is still controversial. A systematic model-oriented review provided a detailed assessment for the role of this pathway in inhibiting the rate of carbon fixation, bridging carbon and nitrogen metabolism, shaping the C1 metabolism, and influencing redox signal transduction. The demand of understanding photosynthesis in its metabolic context calls for the examination of the related processes of the primary carbon metabolism. To this end, the Arabidopsis core model was assembled via a bottom-up approach. This large-scale model can be used to simulate photoautotrophic biomass production, as an indicator for plant growth, under so-called optimal, carbon-limiting and nitrogen-limiting growth conditions. Finally, the introduced model was employed to investigate the effects of the environment, in particular, nitrogen, carbon and energy sources, on the metabolic behavior. This resulted in a purely stoichiometry-based explanation for the experimental evidence for preferred simultaneous acquisition of nitrogen in both forms, as nitrate and ammonium, for optimal growth in various plant species. The findings presented in this thesis provide new insights into plant system's behavior, further support existing opinions for which mounting experimental evidences arise, and posit novel hypotheses for further directed large-scale experiments.
N2  - Mathematische Modellierung biologischer Systeme eröffnet die Möglichkeit systematisch die Funktionsweise biologischer Prozesse und ihrer Wechselwirkungen mit der Umgebung zu untersuchen. Um präzise und biologisch relevante Vorhersagen treffen zu können, muss eine Modellierungsstrategie konzipiert werden, deren Annahmen das untersuchte Szenario bestmöglichst widerspiegelt und die dem Trade-off der Komplexität der zugrunde liegenden mathematischen Beschreibung gerecht wird: Detailtreue gegenüber Größe. Dementsprechend kann das System detailliert, in kleinerem Umfang oder in vereinfachter Darstellung im größeren Maßstab untersucht werden. In dieser Arbeit wird mittels verschiedener Modellierungsansätze, wie kinetischen und stöchiometrischen Modellen, die Rolle der Photosynthese und damit zusammenhängender biochemischer Prozesse im Rahmen des Pflanzenstoffwechsels analysiert. Der Calvin-Benson-Zyklus, als primärer Stoffwechselweg der Kohlenstofffixierung in C3-Pflanzen, ist der erste Schritt der Stärke- und Saccharoseproduktion, welche maßgeblich für das Wachstum von Pflanzen sind. Basierend auf einer integrativen Analyse zur Modellklassifizierung wurden aus der größten bekannten Sammlung von (kinetischen) Modellen des Calvin-Benson-Zyklus diejenigen ermittelt, die für die Entwicklung von Metabolic-Engineering-Strategien geeignet sind. Angeregt von der Fragestellung warum Kohlenstoff transitorisch vorwiegend in Form von Stärke anstatt Saccharose gespeichert wird, wurden die metabolischen Kosten beider Syntheseprozesse genauer betrachtet. Die Einbeziehung der Bereitstellungskosten der beteiligten Enzyme stützt die Tatsache, dass bevorzugt Stärke als temporärer Kohlenstoffspeicher dient. Die entprechende Untersuchung erfolgte einzig auf Grundlage der Stöchiometrie der Synthesewege. In vielen photosynthetisch-aktiven Organismen findet zudem Photorespiration statt, die der Kohlenstofffixierung entgegenwirkt. Die genaue Bedeutung der Photorespiration für den Pflanzenmetabolismus ist noch umstritten. Eine detaillierte Einschätzung der Rolle dieses Stoffwechselweges bezüglich der Inhibierung der Kohlenstofffixierungsrate, der Verknüpfung von Kohlenstoff- und Stickstoffmetabolismus, der Ausprägung des C1-Stoffwechsels sowie die Einflussnahme auf die Signaltransduktion wurde in einer modell-basierten, kritischen Analyse vorgenommen. Um die Photosynthese in ihrem metabolischen Kontext verstehen zu können, ist die Betrachtung der angrenzenden Prozesse des primären Kohlenstoffmetabolismus unverzichtbar. Hierzu wurde in einem Bottom-up Ansatz das Arabidopsis core Modell entworfen, mittels dessen die Biomasseproduktion, als Indikator für Pflanzenwachtum, unter photoautotrophen Bedingungen simuliert werden kann. Neben sogenannten optimalen Wachstumsbedingungen kann dieses großangelegte Modell auch kohlenstoff- und stickstofflimitierende Umweltbedingungen simulieren. Abschließend wurde das vorgestellte Modell zur Untersuchung von Umwelteinflüssen auf das Stoffwechselverhalten herangezogen, im speziellen verschiedene Stickstoff-, Kohlenstoff- und Energiequellen. Diese auschließlich auf der Stöchiometrie basierende Analyse bietet eine Erklärung für die bevorzugte, gleichzeitige Aufnahme von Nitrat und Ammonium, wie sie in verschiedenen Spezies für optimales Wachstum experimentell beobachtet wurde. Die Resultate dieser Arbeit liefern neue Einsichten in das Verhalten von pflanzlichen Systemen, stützen existierende Ansichten, für die zunehmend experimentelle Hinweise vorhanden sind, und postulieren neue Hypothesen für weiterführende großangelegte Experimente.
KW  - stöchiometrische Modellierung
KW  - kinetische Modellierung
KW  - metabolische Netzwerke
KW  - metabolische Kosten
KW  - Photosynthese
KW  - stoichiometric modeling
KW  - kinetic modeling
KW  - metabolic networks
KW  - metabolic costs
KW  - photosynthesis
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-72277
ER  - 
TY  - JOUR
A1  - Arnold, Anne
A1  - Nikoloski, Zoran
T1  - In search for an accurate model of the photosynthetic carbon metabolism
JF  - Mathematics and computers in simulation : transactions of IMACS
N2  - The photosynthetic carbon metabolism, including the Calvin-Benson cycle, is the primary pathway in C-3-plants, producing starch and sucrose from CO2. Understanding the interplay between regulation and efficiency of this pathway requires the development of mathematical models which would explain the observed dynamics of metabolic transformations. Here, we address this question by casting the existing models of Calvin-Benson cycle and the end-product processes into an analysis framework which not only facilitates the comparison of the different models, but also allows for their ranking with respect to chosen criteria, including stability, sensitivity, robustness and/or compliance with experimental data. The importance of the photosynthetic carbon metabolism for the increase of plant biomass has resulted in many models with various levels of detail. We provide the largest compendium of 15 existing, well-investigated models together with a comprehensive classification as well as a ranking framework to determine the best-performing models for metabolic engineering and planning of in silica experiments. The classification can be additionally used, based on the model structure, as a tool to identify the models which match best the experimental design. The provided ranking is just one alternative to score models and, by changing the weighting factor, this framework also could be applied for selection of other criteria of interest.
KW  - Calvin-Benson cycle
KW  - Carbon metabolism
KW  - Model ranking
KW  - Differential and algebraic equations
Y1  - 2014
U6  - https://doi.org/10.1016/j.matcom.2012.03.011
SN  - 0378-4754
SN  - 1872-7166
VL  - 96
SP  - 171
EP  - 194
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - THES
A1  - Arnold, Stefanie
T1  - Epitop-Kartierung von PBP2A und Identifizierung MRSA-spezifischer  immunodominanter Peptidsequenzen
Y1  - 2014
ER  - 
TY  - JOUR
A1  - Attermeyer, Katrin
A1  - Hornick, T.
A1  - Kayler, Z. E.
A1  - Bahr, A.
A1  - Zwirnmann, E.
A1  - Grossart, Hans-Peter
A1  - Premke, K.
T1  - Enhanced bacterial decomposition with increasing addition of autochthonous to allochthonous carbon without any effect on bacterial community composition
JF  - Biogeosciences
N2  - Dissolved organic carbon (DOC) concentrations - mainly of terrestrial origin - are increasing worldwide in inland waters. Heterotrophic bacteria are the main consumers of DOC and thus determine DOC temporal dynamics and availability for higher trophic levels. Our aim was to study bacterial carbon (C) turnover with respect to DOC quantity and chemical quality using both allochthonous and autochthonous DOC sources. We incubated a natural bacterial community with allochthonous C (C-13-labeled beech leachate) and increased concentrations and pulses (intermittent occurrence of organic matter input) of autochthonous C (phytoplankton lysate). We then determined bacterial C consumption, activities, and community composition together with the C flow through bacteria using stable C isotopes. The chemical analysis of single sources revealed differences in aromaticity and low-and high-molecular-weight substance fractions (LMWS and HMWS, respectively) between allochthonous and autochthonous C sources. Both DOC sources (allochthonous and autochthonous DOC) were metabolized at a high bacterial growth efficiency (BGE) around 50%. In treatments with mixed sources, rising concentrations of added autochthonous DOC resulted in a further, significant increase in bacterial DOC consumption of up to 68% when nutrients were not limiting. This rise was accompanied by a decrease in the humic substance (HS) fraction and an increase in bacterial biomass. Changes in DOC concentration and consumption in mixed treatments did not affect bacterial community composition (BCC), but BCC differed in single vs. mixed incubations. Our study highlights that DOC quantity affects bacterial C consumption but not BCC in nutrient-rich aquatic systems. BCC shifted when a mixture of allochthonous and autochthonous C was provided simultaneously to the bacterial community. Our results indicate that chemical quality rather than source of DOC per se (allochthonous vs. autochthonous) determines bacterial DOC turnover.
Y1  - 2014
U6  - https://doi.org/10.5194/bg-11-1479-2014
SN  - 1726-4170
SN  - 1726-4189
VL  - 11
IS  - 6
SP  - 1479
EP  - 1489
PB  - Copernicus
CY  - Göttingen
ER  - 
TY  - JOUR
A1  - Audisio, Paolo
A1  - Cline, Andrew R.
A1  - Solano, Emanuela
A1  - Mancini, Emiliano
A1  - Lamanna, Francesco
A1  - Antonini, Gloria
A1  - Trizzino, Marco
T1  - A peculiar new genus and species of pollen-beetle (Coleoptera, Nitidulidae) from eastern Africa, with a molecular phylogeny of related Meligethinae
JF  - Systematics and biodiversity
KW  - new species
KW  - new genus
KW  - molecular analysis
KW  - pollen-beetles
KW  - host-plants
KW  - Asteraceae
KW  - Kenya
KW  - Tarchonanthopria freidbergi
Y1  - 2014
U6  - https://doi.org/10.1080/14772000.2013.877539
SN  - 1477-2000
SN  - 1478-0933
VL  - 12
IS  - 1
SP  - 77
EP  - 91
PB  - Routledge, Taylor & Francis Group
CY  - Abingdon
ER  - 
TY  - JOUR
A1  - Augusiak, Jacqueline
A1  - Van den Brink, Paul J.
A1  - Grimm, Volker
T1  - Merging validation and evaluation of ecological models to 'evaludation': A review of terminology and a practical approach
JF  - Ecological modelling : international journal on ecological modelling and engineering and systems ecolog
N2  - Confusion about model validation is one of the main challenges in using ecological models for decision support, such as the regulation of pesticides. Decision makers need to know whether a model is a sufficiently good representation of its real counterpart and what criteria can be used to answer this question. Unclear terminology is one of the main obstacles to a good understanding of what model validation is, how it works, and what it can deliver. Therefore, we performed a literature review and derived a standard set of terms. 'Validation' was identified as a catch-all term, which is thus useless for any practical purpose. We introduce the term 'evaludation', a fusion of 'evaluation' and 'validation', to describe the entire process of assessing a model's quality and reliability. Considering the iterative nature of model development, the modelling cycle, we identified six essential elements of evaludation: (i) 'data evaluation' for scrutinising the quality of numerical and qualitative data used for model development and testing; (ii) 'conceptual model evaluation' for examining the simplifying assumptions underlying a model's design; (iii) 'implementation verification' for testing the model's implementation in equations and as a computer programme; (iv) 'model output verification' for comparing model output to data and patterns that guided model design and were possibly used for calibration; (v) 'model analysis' for exploring the model's sensitivity to changes in parameters and process formulations to make sure that the mechanistic basis of main behaviours of the model has been well understood; and (vi) 'model output corroboration' for comparing model output to new data and patterns that were not used for model development and parameterisation. Currently, most decision makers require 'validating' a model by testing its predictions with new experiments or data. Despite being desirable, this is neither sufficient nor necessary for a model to be useful for decision support. We believe that the proposed set of terms and its relation to the modelling cycle can help to make quality assessments and reality checks of ecological models more comprehensive and transparent. (C) 2013 Elsevier B.V. All rights reserved.
KW  - Model validation
KW  - Terminology
KW  - Decision support
KW  - Documentation
KW  - Ecological models
KW  - Risk assessment
Y1  - 2014
U6  - https://doi.org/10.1016/j.ecolmodel.2013.11.009
SN  - 0304-3800
SN  - 1872-7026
VL  - 280
SP  - 117
EP  - 128
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Azuma, Yusuke
A1  - Kuekenshoener, Tim
A1  - Ma, Guangyong
A1  - Yasunaga, Jun-ichiro
A1  - Imanishi, Miki
A1  - Tanaka, Gen
A1  - Nakase, Ikuhiko
A1  - Maruno, Takahiro
A1  - Kobayashi, Yuji
A1  - Arndt, Katja Maren
A1  - Matsuoka, Masao
A1  - Futaki, Shiroh
T1  - Controlling leucine-zipper partner recognition in cells through modification of a-g interactions
JF  - Chemical communications
N2  - By focusing on the a-g interactions, successful design and selection were accomplished to obtain a leucine-zipper segment that discriminates the appropriate partner over another that provides very similar patterns of electrostatic interactions.
Y1  - 2014
U6  - https://doi.org/10.1039/c4cc00555d
SN  - 1359-7345
SN  - 1364-548X
VL  - 50
IS  - 48
SP  - 6364
EP  - 6367
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - GEN
A1  - Azuma, Yusuke
A1  - Kükenshöner, Tim
A1  - Ma, Guangyong
A1  - Yasunaga, Jun-ichiro
A1  - Imanishi, Miki
A1  - Tanaka, Gen
A1  - Nakase, Ikuhiko
A1  - Maruno, Takahiro
A1  - Kobayashi, Yuji
A1  - Arndt, Katja Maren
A1  - Matsuoka, Masao
A1  - Futaki, Shiroh
T1  - Controlling leucine-zipper partner recognition in cells through modification of a–g interactions
N2  - By focusing on the a–g interactions, successful design and selection were accomplished to obtain a leucine-zipper segment that discriminates the appropriate partner over another that provides very similar patterns of electrostatic interactions.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 276 
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-98758
ER  - 
TY  - JOUR
A1  - Badalyan, Artavazd
A1  - Dierich, Marlen
A1  - Stiba, Konstanze
A1  - Schwuchow, Viola
A1  - Leimkühler, Silke
A1  - Wollenberger, Ulla
T1  - Electrical wiring of the aldehyde oxidoreductase PaoABC with a polymer containing osmium redox centers
BT  - biosensors for benzaldehyde and GABA
JF  - Biosensors
N2  - Biosensors for the detection of benzaldehyde and g-aminobutyric acid (GABA) are reported using aldehyde oxidoreductase PaoABC from Escherichia coli immobilized in a polymer containing bound low potential osmium redox complexes. The electrically connected enzyme already electrooxidizes benzaldehyde at potentials below −0.15 V (vs. Ag|AgCl, 1 M KCl). The pH-dependence of benzaldehyde oxidation can be strongly influenced by the ionic strength. The effect is similar with the soluble osmium redox complex and therefore indicates a clear electrostatic effect on the bioelectrocatalytic efficiency of PaoABC in the osmium containing redox polymer. At lower ionic strength, the pH-optimum is high and can be switched to low pH-values at high ionic strength. This offers biosensing at high and low pH-values. A “reagentless” biosensor has been formed with enzyme wired onto a screen-printed electrode in a flow cell device. The response time to addition of benzaldehyde is 30 s, and the measuring range is between 10–150 µM and the detection limit of 5 µM (signal to noise ratio 3:1) of benzaldehyde. The relative standard deviation in a series (n = 13) for 200 µM benzaldehyde is 1.9%. For the biosensor, a response to succinic semialdehyde was also identified. Based on this response and the ability to work at high pH a biosensor for GABA is proposed by coimmobilizing GABA-aminotransferase (GABA-T) and PaoABC in the osmium containing redox polymer.
KW  - redox polymer
KW  - aldehyde oxidoreductase
KW  - ionic strength
KW  - benzaldehyde
KW  - GABA
KW  - biosensor
Y1  - 2014
U6  - https://doi.org/10.3390/bios4040403
VL  - 4
IS  - 4
SP  - 403
EP  - 421
PB  - MDPI
CY  - Basel
ER  - 
TY  - GEN
A1  - Badalyan, Artavazd
A1  - Dierich, Marlen
A1  - Stiba, Konstanze
A1  - Schwuchow, Viola
A1  - Leimkühler, Silke
A1  - Wollenberger, Ulla
T1  - Electrical wiring of the aldehyde oxidoreductase PaoABC with a polymer containing osmium redox centers
BT  - biosensors for benzaldehyde and GABA
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Biosensors for the detection of benzaldehyde and g-aminobutyric acid (GABA) are reported using aldehyde oxidoreductase PaoABC from Escherichia coli immobilized in a polymer containing bound low potential osmium redox complexes. The electrically connected enzyme already electrooxidizes benzaldehyde at potentials below −0.15 V (vs. Ag|AgCl, 1 M KCl). The pH-dependence of benzaldehyde oxidation can be strongly influenced by the ionic strength. The effect is similar with the soluble osmium redox complex and therefore indicates a clear electrostatic effect on the bioelectrocatalytic efficiency of PaoABC in the osmium containing redox polymer. At lower ionic strength, the pH-optimum is high and can be switched to low pH-values at high ionic strength. This offers biosensing at high and low pH-values. A “reagentless” biosensor has been formed with enzyme wired onto a screen-printed electrode in a flow cell device. The response time to addition of benzaldehyde is 30 s, and the measuring range is between 10–150 µM and the detection limit of 5 µM (signal to noise ratio 3:1) of benzaldehyde. The relative standard deviation in a series (n = 13) for 200 µM benzaldehyde is 1.9%. For the biosensor, a response to succinic semialdehyde was also identified. Based on this response and the ability to work at high pH a biosensor for GABA is proposed by coimmobilizing GABA-aminotransferase (GABA-T) and PaoABC in the osmium containing redox polymer.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1082 
KW  - redox polymer
KW  - aldehyde oxidoreductase
KW  - ionic strength
KW  - benzaldehyde
KW  - GABA
KW  - biosensor
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-475070
SN  - 1866-8372
IS  - 1082
ER  - 
TY  - JOUR
A1  - Baeten, Lander
A1  - Warton, David I.
A1  - Van Calster, Hans
A1  - De Frenne, Pieter
A1  - Verstraeten, Gorik
A1  - Bonte, Dries
A1  - Bernhardt-Römermann, Markus
A1  - Cornelis, Johnny
A1  - Decocq, Guillaume
A1  - Eriksson, Ove
A1  - Hedl, Radim
A1  - Heinken, Thilo
A1  - Hermy, Martin
A1  - Hommel, Patrick
A1  - Kirby, Keith J.
A1  - Naaf, Tobias
A1  - Petrik, Petr
A1  - Walther, Gian-Reto
A1  - Wulf, Monica
A1  - Verheyen, Kris
T1  - A model-based approach to studying changes in compositional heterogeneity
JF  - Methods in ecology and evolution : an official journal of the British Ecological Society
Y1  - 2014
SN  - 2041-210X
SN  - 2041-2096
VL  - 5
IS  - 2
SP  - 156
EP  - 164
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - INPR
A1  - Balazadeh, Salma
T1  - Stay-green not always stays green
T2  - Molecular plant
Y1  - 2014
U6  - https://doi.org/10.1093/mp/ssu076
SN  - 1674-2052
SN  - 1752-9867
VL  - 7
IS  - 8
SP  - 1264
EP  - 1266
PB  - Cell Press
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Balazadeh, Salma
A1  - Schildhauer, Joerg
A1  - Araujo, Wagner L.
A1  - Munne-Bosch, Sergi
A1  - Fernie, Alisdair R.
A1  - Proost, Sebastian
A1  - Humbeck, Klaus
A1  - Müller-Röber, Bernd
T1  - Reversal of senescence by N resupply to N-starved Arabidopsis thaliana: transcriptomic and metabolomic consequences
JF  - Journal of experimental botany
N2  - Leaf senescence is a developmentally controlled process, which is additionally modulated by a number of adverse environmental conditions. Nitrogen shortage is a well-known trigger of precocious senescence in many plant species including crops, generally limiting biomass and seed yield. However, leaf senescence induced by nitrogen starvation may be reversed when nitrogen is resupplied at the onset of senescence. Here, the transcriptomic, hormonal, and global metabolic rearrangements occurring during nitrogen resupply-induced reversal of senescence in Arabidopsis thaliana were analysed. The changes induced by senescence were essentially in keeping with those previously described; however, these could, by and large, be reversed. The data thus indicate that plants undergoing senescence retain the capacity to sense and respond to the availability of nitrogen nutrition. The combined data are discussed in the context of the reversibility of the senescence programme and the evolutionary benefit afforded thereby. Future prospects for understanding and manipulating this process in both Arabidopsis and crop plants are postulated.
KW  - Arabidopsis
KW  - gene expression
KW  - metabolomics
KW  - nitrogen limitation
KW  - senescence
KW  - transcriptome
Y1  - 2014
U6  - https://doi.org/10.1093/jxb/eru119
SN  - 0022-0957
SN  - 1460-2431
VL  - 65
IS  - 14
SP  - 3975
EP  - 3992
PB  - Oxford Univ. Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Basel, Nicolai
A1  - Harms, Ute
A1  - Prechtl, Helmut
A1  - Weiss, Thomas
A1  - Rothgangel, Martin
T1  - Students' arguments on the science and religion issue: the example of evolutionary theory and Genesis
JF  - Journal of biological education
N2  - Treating creationism as a controversial topic within the science and religion issue in the science classroom has been widely discussed in the recent literature. Some researchers have proposed that this topic is best addressed by focusing on sociocognitive conflict. To prepare new learning opportunities for this approach, it is necessary to know the concrete arguments that students use in their discussions on this issue. Therefore, this study aimed to provide a systematic description of these arguments. For this purpose, upper secondary students (N=43) argued for either the acceptance of evolutionary theory or faith in Genesis in a written speech. The study was conducted during their regular biology and religious education classes. Generated arguments were analysed by qualitative content analysis. Three dimensions of the arguments were described: the content (science or religion), the valuation of the argument (positive or negative), and whether the argument consisted of a descriptive or normative argumentation. The results indicate that students found it easier to generate arguments about the scientific side of the issue; however, these arguments were negatively constructed. The results are discussed with regard to implications for educational approaches for teaching controversial issues at the high-school level.
KW  - evolutionary theory
KW  - argumentation
KW  - science
KW  - religion issue
KW  - controversial issues
Y1  - 2014
U6  - https://doi.org/10.1080/00219266.2013.849286
SN  - 0021-9266
SN  - 2157-6009
VL  - 48
IS  - 4
SP  - 179
EP  - 187
PB  - Routledge, Taylor & Francis Group
CY  - Abingdon
ER  - 
TY  - JOUR
A1  - Bauer, Barbara
A1  - Vos, Matthijs
A1  - Klauschies, Toni
A1  - Gaedke, Ursula
T1  - Diversity, functional similarity, and top-down control drive synchronization and the reliability of ecosystem function
JF  - The American naturalist : a bi-monthly journal devoted to the advancement and correlation of the biological sciences
N2  - The concept that diversity promotes reliability of ecosystem function depends on the pattern that community-level biomass shows lower temporal variability than species-level biomasses. However, this pattern is not universal, as it relies on compensatory or independent species dynamics. When in contrast within--trophic level synchronization occurs, variability of community biomass will approach population-level variability. Current knowledge fails to integrate how species richness, functional distance between species, and the relative importance of predation and competition combine to drive synchronization at different trophic levels. Here we clarify these mechanisms. Intense competition promotes compensatory dynamics in prey, but predators may at the same time increasingly synchronize, under increasing species richness and functional similarity. In contrast, predators and prey both show perfect synchronization under strong top-down control, which is promoted by a combination of low functional distance and high net growth potential of predators. Under such conditions, community-level biomass variability peaks, with major negative consequences for reliability of ecosystem function.
KW  - biodiversity
KW  - ecosystem services
KW  - population dynamics
KW  - predator-prey system
KW  - species richness
KW  - synchrony
Y1  - 2014
U6  - https://doi.org/10.1086/674906
SN  - 0003-0147
SN  - 1537-5323
VL  - 183
IS  - 3
SP  - 394
EP  - 409
PB  - Univ. of Chicago Press
CY  - Chicago
ER  - 
TY  - JOUR
A1  - Becher, Matthias A.
A1  - Grimm, Volker
A1  - Thorbek, Pernille
A1  - Horn, Juliane
A1  - Kennedy, Peter J.
A1  - Osborne, Juliet L.
T1  - BEEHAVE: a systems model of honeybee colony dynamics and foraging to explore multifactorial causes of colony failure
JF  - Journal of applied ecology : an official journal of the British Ecological Society
N2  - BEEHAVE offers a valuable tool for researchers to design and focus field experiments, for regulators to explore the relative importance of stressors to devise management and policy advice and for beekeepers to understand and predict varroa dynamics and effects of management interventions. We expect that scientists and stakeholders will find a variety of applications for BEEHAVE, stimulating further model development and the possible inclusion of other stressors of potential importance to honeybee colony dynamics.
KW  - Apis mellifera
KW  - colony decline
KW  - cross-level interactions
KW  - feedbacks
KW  - foraging
KW  - modelling
KW  - multiple stressors
KW  - multi-agent simulation
KW  - predictive systems ecology
KW  - Varroa destructor
Y1  - 2014
U6  - https://doi.org/10.1111/1365-2664.12222
SN  - 0021-8901
SN  - 1365-2664
VL  - 51
IS  - 2
SP  - 470
EP  - 482
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Bechi, Beatrice
A1  - Herter, Susanne
A1  - McKenna, Shane
A1  - Riley, Christopher
A1  - Leimkühler, Silke
A1  - Turner, Nicholas J.
A1  - Carnell, Andrew J.
T1  - Catalytic bio-chemo and bio-bio tandem oxidation reactions for amide and carboxylic acid synthesis
JF  - Green chemistry : an international journal and green chemistry resource
N2  - A catalytic toolbox for three different water-based one-pot cascades to convert aryl alcohols to amides and acids and cyclic amines to lactams, involving combination of oxidative enzymes (monoamine oxidase, xanthine dehydrogenase, galactose oxidase and laccase) and chemical oxidants (TBHP or Cul(cat)/H2O2) at mild temperatures, is presented. Mutually compatible conditions were found to afford products in good to excellent yields.
Y1  - 2014
U6  - https://doi.org/10.1039/c4gc01321b
SN  - 1463-9262
SN  - 1463-9270
VL  - 16
IS  - 10
SP  - 4524
EP  - 4529
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  -