TY  - JOUR
A1  - Paijmans, Johanna L. A.
A1  - Barlow, Axel
A1  - Henneberger, Kirstin
A1  - Fickel, Jörns
A1  - Hofreiter, Michael
A1  - Foerste, Daniel W. G.
T1  - Ancestral mitogenome capture of the Southeast Asian banded linsang
JF  - PLoS ONE
N2  - Utilising a reconstructed ancestral mitochondrial genome of a clade to design hybridisation capture baits can provide the opportunity for recovering mitochondrial sequences from all its descendent and even sister lineages. This approach is useful for taxa with no extant close relatives, as is often the case for rare or extinct species, and is a viable approach for the analysis of historical museum specimens. Asiatic linsangs (genus Prionodon) exemplify this situation, being rare Southeast Asian carnivores for which little molecular data is available. Using ancestral capture we recover partial mitochondrial genome sequences for seven banded linsangs (P. linsang) from historical specimens, representing the first intraspecific genetic dataset for this species. We additionally assemble a high quality mitogenome for the banded linsang using shotgun sequencing for time-calibrated phylogenetic analysis. This reveals a deep divergence between the two Asiatic linsang species (P. linsang, P. pardicolor), with an estimated divergence of ~12 million years (Ma). Although our sample size precludes any robust interpretation of the population structure of the banded linsang, we recover two distinct matrilines with an estimated tMRCA of ~1 Ma. Our results can be used as a basis for further investigation of the Asiatic linsangs, and further demonstrate the utility of ancestral capture for studying divergent taxa without close relatives.
KW  - Shotgun sequencing
KW  - Mitochondria
KW  - Phylogenetics
KW  - Phylogenetic analysis
KW  - Paleogenetics
KW  - Sequence alignment
KW  - Genomics
KW  - Museum collections
Y1  - 2019
U6  - https://doi.org/10.1371/journal.pone.0234385
SN  - 1932-6203
VL  - 15
IS  - 6
PB  - PLOS
CY  - San Francisco, California, US
ER  - 
TY  - JOUR
A1  - Gurke, Marie
A1  - Vidal-Gorosquieta, Amalia
A1  - Pajimans, Johanna L. A.
A1  - Wȩcek, Karolina
A1  - Barlow, Axel
A1  - González-Fortes, Gloria M.
A1  - Hartmann, Stefanie
A1  - Grandal-d’Anglade, Aurora
A1  - Hofreiter, Michael
T1  - Insight into the introduction of domestic cattle and the process of Neolithization to the Spanish region Galicia by genetic evidence
JF  - PLoS ONE
N2  - Domestic cattle were brought to Spain by early settlers and agricultural societies. Due to missing Neolithic sites in the Spanish region of Galicia, very little is known about this process in this region. We sampled 18 cattle subfossils from different ages and different mountain caves in Galicia, of which 11 were subject to sequencing of the mitochondrial genome and phylogenetic analysis, to provide insight into the introduction of cattle to this region. We detected high similarity between samples from different time periods and were able to compare the time frame of the first domesticated cattle in Galicia to data from the connecting region of Cantabria to show a plausible connection between the Neolithization of these two regions. Our data shows a close relationship of the early domesticated cattle of Galicia and modern cow breeds and gives a general insight into cattle phylogeny. We conclude that settlers migrated to this region of Spain from Europe and introduced common European breeds to Galicia.
KW  - Haplogroups
KW  - Mitochondria
KW  - Cattle
KW  - Genomics
KW  - Domestic animals
KW  - Livestock
KW  - Single nucleotide polymorphisms
KW  - Neolithic period
Y1  - 2020
U6  - https://doi.org/10.1371/journal.pone.0249537
SN  - 1932-6203
VL  - 16
IS  - 4
PB  - Public Library of Science
CY  - San Francisco
ER  -