TY  - JOUR
A1  - Müller, Marik
A1  - Nedielkov, Ruslan
A1  - Arndt, Katja M.
T1  - Strategies for Enzymatic Inactivation of the Veterinary  Antibiotic Florfenicol
JF  - Antibiotics
N2  - Large quantities of the antibiotic florfenicol are used in animal farming and aquaculture, contaminating the ecosystem with antibiotic residues and promoting antimicrobial resistance, ultimately leading to untreatable multidrug-resistant pathogens. Florfenicol-resistant bacteria often activate export mechanisms that result in resistance to various structurally unrelated antibiotics. We devised novel strategies for the enzymatic inactivation of florfenicol in different media, such as saltwater or milk. Using a combinatorial approach and selection, we optimized a hydrolase (EstDL136) for florfenicol cleavage. Reaction kinetics were followed by time-resolved NMR spectroscopy. Importantly, the hydrolase remained active in different media, such as saltwater or cow milk. Various environmentally-friendly application strategies for florfenicol inactivation were developed using the optimized hydrolase. As a potential filter device for cost-effective treatment of waste milk or aquacultural wastewater, the hydrolase was immobilized on Ni-NTA agarose or silica as carrier materials. In two further application examples, the hydrolase was used as cell extract or encapsulated with a semi-permeable membrane. This facilitated, for example, florfenicol inactivation in whole milk, which can help to treat waste milk from medicated cows, to be fed to calves without the risk of inducing antibiotic resistance. Enzymatic inactivation of antibiotics, in general, enables therapeutic intervention without promoting antibiotic resistance.
KW  - aquaculture
KW  - antibiotic inactivation
KW  - enzyme optimization
KW  - enzymatic inactivation
KW  - florfenicol
KW  - immobilization
KW  - industrial farming
Y1  - 2022
U6  - https://doi.org/10.3390/antibiotics11040443
SN  - 2079-6382
VL  - 11
IS  - 4
SP  - 1
EP  - 18
PB  - MDPI
CY  - Basel, Schweiz
ER  -