TY  - JOUR
A1  - Hille, Carsten
A1  - Berg, Maik
A1  - Bressel, Lena
A1  - Munzke, Dorit
A1  - Primus, Philipp
A1  - Löhmannsröben, Hans-Gerd
A1  - Dosche, Carsten
T1  - Time-domain fluorescence lifetime imaging for intracellular pH sensing in living tissues
N2  - pH sensing in living cells represents one of the most prominent topics in biochemistry and physiology. In this study we performed one-photon and two-photon time-domain fluorescence lifetime imaging with a laser-scanning microscope using the time-correlated single-photon counting technique for imaging intracellular pH levels. The suitability of different commercial fluorescence dyes for lifetime-based pH sensing is discussed on the basis of in vitro as well of in situ measurements. Although the tested dyes are suitable for intensity-based ratiometric measurements, for lifetime- based techniques in the time-domain so far only BCECF seems to meet the requirements of reliable intracellular pH recordings in living cells.
Y1  - 2008
U6  - https://doi.org/10.1007/s00216-008-2147-0
ER  -