TY  - JOUR
A1  - Garcia, Ada
A1  - Rühl, Ralph
A1  - Herz, U.
A1  - Koebnick, Corinna
A1  - Schweigert, Florian J.
A1  - Worm, Margitta
T1  - Retinoid- and carotenoid-enriched diets influence the ontogenesis of the immune system in mice
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Rühl, Ralph
A1  - Schweigert, Florian J.
T1  - Automated solid-phase extraction and liquid chromatographic method for retinoid determination in biological samples
N2  - In this study, a method for partly automated sample preparation and fully automated solid-phase extraction method for plasma, kidney and liver samples for various retinoids like all-trans-4-oxo-retinoic acid, 13-cis-4-oxo- retinoic acid, 13-cis-retinoic acid, 9-cis-retinoic acid, all-trans-retinoic acid, retinol and retinyl palmitate was established. Plasma, embryo-, kidney-and liver-homogenates were automatically mixed and extracted on multiple usage solid-phase (C2) extraction cartridges immediately before HPLC analysis. Automated cleaning, preconditioning and incorporation of the loaded cartridge to fully automated HPLC separation and quantification of the various retinoids in a single HPLC run was established. The recovery of the retinoids was generally between 80 and 90%. Intra-day repeatability was <11.7%. As little as 1.2 ng/ml could be quantified in lipid-mixture standard samples. This method allows a highly automated sample preparation and a fully automated solid-phase extraction with good selectivity for the study of endogenous retinoids and retinoids after nutritional supplementations and pharmacological applications in several biological samples. (C) 2003 Elsevier B.V. All rights reserved
Y1  - 2003
ER  -