TY  - JOUR
A1  - Nießner, Reinhard
A1  - Broekaert, J.
A1  - Einax, J. W.
A1  - Scheller, Frieder W.
A1  - Stöcklein, Walter F. M.
T1  - Trendbericht analytische Biochemie 2000/2001
Y1  - 2002
ER  - 
TY  - JOUR
A1  - Himmel, Mirko
A1  - VanderVen, Peter F. M.
A1  - Stöcklein, Walter F. M.
A1  - Fürst, Dieter Oswald
T1  - The limits of promiscuity : isoform-specific dimerization of filamins
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Halámek, Jan
A1  - Wollenberger, Ursula
A1  - Stöcklein, Walter F. M.
A1  - Warsinke, Axel
A1  - Scheller, Frieder W.
T1  - Signal amplification in immunoassays using labeling via boronic acid binding to the sugar moiety of immunoglobulin G : proof of concept for glycated hemoglobin
N2  - A novel electrochemical immunoassay based on the multiple affinity labeling of the indicator antibody with an electro-active tag is presented. The concept is illustrated for the determination of the glycated hemoglobin HbA1c in hemoglobin samples. Hemoglobin is adsorbed to the surfactant-modified surface of a piezoelectric quartz crystal. Whereas the quartz crystal nanobalance is used to validate the total Hb binding, the HbA1c on the sensor surface is recognized by an antibody and quantified electrochemically after the sugar moieties of the antibody have been labeled in-situ with ferroceneboronic acid. The sensitivity of this sensor is about threefold higher than the sensitivity of a hemoglobin sensor, where the ferroceneboronic acid is bound directly to HbA1c.
Y1  - 2007
UR  - http://www.informaworld.com/openurl?genre=journal&issn=0003-2719
U6  - https://doi.org/10.1080/00032710701327096
SN  - 0003-2719
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Rohde, M.
A1  - Scharte, Gudrun
A1  - Behrsing, Olaf
A1  - Warsinke, Axel
A1  - Micheel, Burkhard
A1  - Scheller, Frieder W.
T1  - Sensitive detection of triazine and phenylurea pesticides in pure organic solvent by enzyme linked immunsorbent assay (ELISA): stabilities, solubilities and sensitives
Y1  - 2000
ER  - 
TY  - JOUR
A1  - Neumann, Meina
A1  - Schulte, Marc
A1  - Jünemann, Nora
A1  - Stöcklein, Walter F. M.
A1  - Leimkühler, Silke
T1  - Rhodobacter capsulatus XdhC is involved in molybdenum cofactor binding and insertion into xanthine dehydrogenase
N2  - Rhodobacter capsulatus xanthine dehydrogenase (XDH) is a cytoplasmic enzyme with an (alpha beta) 2 heterodimeric structure that is highly identical to homodimeric eukaryotic xanthine oxidoreductases. The crystal structure revealed that the molybdenum cofactor (Moco) is deeply buried within the protein. A protein involved in Moco insertion and XDH maturation has been identified, which was designated XdhC. XdhC was shown to be essential for the production of active XDH but is not a subunit of the purified enzyme. Here we describe the purification of XdhC and the detailed characterization of its role for XDH maturation. We could show that XdhC binds Moco in stoichiometric amounts, which subsequently can be inserted into Moco-free apo-XDH. A specific interaction between XdhC and XdhB was identified. We show that XdhC is required for the stabilization of the sulfurated form of Moco present in enzymes of the xanthine oxidase family. Our findings imply that enzyme-specific proteins exist for the biogenesis of molybdoenzymes, coordinating Moco binding and insertion into their respective target proteins. So far, the requirement of such proteins for molybdoenzyme maturation has been described only for prokaryotes
Y1  - 2006
UR  - http://www.jbc.org/
U6  - https://doi.org/10.1074/jbc.M601617200
ER  - 
TY  - JOUR
A1  - Stech, Marlitt
A1  - Merk, Helmut
A1  - Schenk, Jörg A.
A1  - Stöcklein, Walter F. M.
A1  - Wüstenhagen, Doreen Anja
A1  - Micheel, Burkhard
A1  - Duschl, Claus
A1  - Bier, Frank Fabian
A1  - Kubick, Stefan
T1  - Production of functional antibody fragments in a vesicle-based eukaryotic cell-free translation system
JF  - Journal of biotechnology
N2  - Cell-free protein synthesis is of increasing interest for the rapid and high-throughput synthesis of many proteins, in particular also antibody fragments. In this study, we present a novel strategy for the production of single chain antibody fragments (scFv) in a eukaryotic in vitro translation system. This strategy comprises the cell-free expression, isolation and label-free interaction analysis of a model antibody fragment synthesized in two differently prepared insect cell lysates. These lysates contain translocationally active microsomal structures derived from the endoplasmic reticulum (ER), allowing for posttranslational modifications of cell-free synthesized proteins. Both types of these insect cell lysates enable the synthesis and translocation of scFv into ER-derived vesicles. However, only the one that has a specifically adapted redox potential yields functional active antibody fragments. We have developed a new methodology for the isolation of functional target proteins based on the translocation of cell-free produced scFv into microsomal structures and subsequent collection of protein-enriched vesicles. Antibody fragments that have been released from these vesicles are shown to be well suited for label-free binding studies. Altogether, these results show the potential of insect cell lysates for the production, purification and selection of antibody fragments in an easy-to-handle and time-saving manner.
KW  - Cell-free
KW  - In vitro translation
KW  - Single chain antibody (scFv)
KW  - Insect lysate
KW  - Surface plasmon resonance
Y1  - 2012
U6  - https://doi.org/10.1016/j.jbiotec.2012.08.020
SN  - 0168-1656
VL  - 164
IS  - 2
SP  - 220
EP  - 231
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Kleuser, U.
A1  - Stöcklein, Walter F. M.
A1  - Pieper-Fürst, U.
A1  - Scheller, Frieder W.
T1  - Partikelverstärkte Oberflächenplasmonresonanz für die Quantifizierung von Matrix Metalloproteinase-2
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Warsinke, Axel
A1  - Scheller, Frieder W.
T1  - Organic solvent modified enzyme-liked immunoassay for the detection of triazine herbicides
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Nitsche, Andreas
A1  - Kurth, Andreas
A1  - Dunkhorst, Anna
A1  - Pänke, Oliver
A1  - Sielaff, Hendrik
A1  - Junge, Wolfgang
A1  - Muth, Doreen
A1  - Scheller, Frieder W.
A1  - Stöcklein, Walter F. M.
A1  - Pauli, Georg
A1  - Kage, Andreas
T1  - One-step selection of vaccinia virus binding DNA-aptamers by MonoLEX
Y1  - 2007
UR  - http://www.biomedcentral.com/1472-6750/7
U6  - https://doi.org/10.1186/1472-6750-7-48
ER  - 
TY  - CHAP
A1  - Memczak, Henry
A1  - Lauster, Daniel
A1  - Herrmann, Andreas
A1  - Stöcklein, Walter F. M.
A1  - Bier, Frank Fabian
T1  - Novel hemagglutinin-binding peptides for biosensing and inhibition of Influenza Viruses
T2  - Biopolymers
Y1  - 2013
SN  - 0006-3525
SN  - 1097-0282
VL  - 100
IS  - 3
SP  - 255
EP  - 255
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
T1  - Molecule-detective
BT  - Molekül-Detektive : Biosensoren
N2  - Biosensors are analytical devices incorporating biological material (receptor) intimately associated with or integrated within a physicochernical transducer. Advantages are the high selectivity for analyte detection. Examples given comprise the very successful commercial blood glucose biosensors made for the self-control by the diabetic patients. Other biosensors are part of an analytic system, including the sensor chips Of surface plasmon resonance or interferometry based devices, piezoelectric or reflectometric sensors capable of direct measurement of mass changes, and thermometric and other reagentless sensors. The development of nanotubes-based devices allows for significant enhancment of the signal-to-noise ratio of the biosensors. A milestone on the way towards miniaturization and parallelization of biosensors is the recently developed and prize-winning electronic DNA chip
Y1  - 2006
ER  - 
TY  - JOUR
A1  - Kramer, A.
A1  - Keitel, T.
A1  - Winkler, K.
A1  - Stöcklein, Walter F. M.
A1  - Hühne, Wolfgang
A1  - Schneider-Mergener, Jens
T1  - Molecular basis for the binding promiscuity of an anti-P24 (HIV-1) monoclonal antibody
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Stöllner, Daniela
A1  - Stöcklein, Walter F. M.
A1  - Scheller, Frieder W.
A1  - Warsinke, Axel
T1  - Membrane-immobilized haptoglobin as affinity matrix for a hemoglobin-A1c-immunosensor
Y1  - 2002
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Scheller, Frieder W.
T1  - Laccase : a marker enzyme for solvent modified immunoassays
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Schenk, Jörg A.
A1  - Sellrie, Frank
A1  - Böttger, Volker
A1  - Micheel, Burkhard
A1  - Stöcklein, Walter F. M.
T1  - Generation and application of a fluorescein-specific single chain antibody
N2  - A recombinant single chain antibody fragment (designated scDE1) of the murine monoclonal anti-fluorescein antibody B13-DE1 was generated using the original hybridoma cells as source for the variable antibody heavy and light chain (VH and VL) genes. After cloning the variable genes into a phage vector a functional antibody fragment was selected by phage display panning. Recombinant antibody could be expressed as phage antibody and as soluble single chain antibody in Escherichia coli. High yield of scDE1 could also be detected in bacterial culture supernatant. The scDE1 showed the same binding specificity as the parental monoclonal antibody, i.e. it bound fluorescein, fluorescein derivatives and a fluorescein peptide mimotope. Surface plasmon resonance revealed a K(D) of 19 nM for the scDE1 compared to 0.7 nM for the monoclonal antibody. The isolated soluble scDE1 could easily be conjugated to horseradish peroxidase which allowed the use of the conjugate as universal indicator for the detection of fluorescein-labelled proteins in different immunoassays. Detection of hCG in urine was performed as a model system using scDE1. In addition to E. coli the scFv genes could also be transferred and expressed in eukaryotic cells. Finally, we generated HEK293 cells expressing the scDE1 at the cell surface.
Y1  - 2007
UR  - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VRJ-4P3DY33- 1&_user=1584062&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000053886&_version=1&_urlVersion=0&_userid=1584062&md5=e 4
ER  - 
TY  - JOUR
A1  - Wollenberger, Ursula
A1  - Jin, Wen
A1  - Bernhardt, Rita
A1  - Lehmann, Claudia
A1  - Stöcklein, Walter F. M.
A1  - Brigelius-Flohé, Regina
A1  - Scheller, Frieder W.
T1  - Funktionalisierung von Elektroden für den direkten heterogenen Elektrotransfer
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Scheller, Frieder W.
T1  - Enzymes and antibodies in organic media : analytical applications
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Makower, Alexander
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - Enzyme sensors and enzyme amplifification systems
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Behrsing, Olaf
A1  - Scharte, Gudrun
A1  - Micheel, Burkhard
A1  - Benkert, Alexander
A1  - Schössler, W.
A1  - Warsinke, Axel
A1  - Scheller, Frieder W.
T1  - Enzyme kinetic assays with surface plasmon resonance (BIAcore) based on competition between enzyme and creatinine antibody
Y1  - 2000
ER  - 
TY  - JOUR
A1  - Chen, Jian
A1  - Stöcklein, Walter F. M.
A1  - Scheller, Frieder W.
A1  - Wollenberger, Ursula
T1  - Electrochemical determination of human hemoglobin by using ferrocene carboxylic acid modified carbon powder microelectrode
Y1  - 2003
ER  -