TY  - JOUR
A1  - Warncke, Max
A1  - Vogt, Birgit
A1  - Ulrich, Jacqueline
A1  - von Laer, Meike Dorothee
A1  - Beyer, Winfried
A1  - Klump, Hannes
A1  - Micheel, Burkhard
A1  - Sheriff, Ahmed
T1  - Efficient in vitro transduction of naive murine B cells with lentiviral vectors
N2  - The aim of this study was to determine the impact of lentiviral transduction on primary murine B cells. Studying B cell activities in vivo or using them for tolerance induction requires that the cells remain unaltered in their biological behavior except for expression of the transgene. As we show here, murine B cells can efficiently be transduced by lentiviral, VSV-G-pseudotyped vectors without the necessity of prior activation. Culture with LPS gave enhanced transduction efficiencies but led to the upregulation of CD86 and proliferation of the cells. Transduction of naive B cells by lentiviral vectors was dependent on multiplicity of infection and did not lead to a concomitant activation. Furthermore, the transduced cells could be used for studies in the NOD mouse system without altering the onset of diabetes. We conclude that lentiviral gene transfer into naive B cells is a powerful tool for manipulation of B cells for therapeutic applications.
Y1  - 2004
UR  - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4C707VR- 7&_coverDate=06%2F04%2F2004&_alid=269000954&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6713&_sort=d&view=c&_acct=C000053886&_v e
ER  - 
TY  - JOUR
A1  - Vogt, Birgit
A1  - Warncke, Marit
A1  - Micheel, Burkhard
A1  - Sheriff, Ahmed
T1  - Lentiviral gene transfer of CTLA4 generates B cells with reduced costimulatory properties : brief definite report
N2  - Peripheral T-cell (TC) tolerance can be induced by tolerogenic antigen-presenting cell (APC). A prerequisite is the reduction or blockade of B7 of APC. Besides dendritic cell, B cells can be used as APC. Here, we show the generation B cells with reduced B7 expression by lentiviral transduction of endoplasmic reticulum (ER)-directed CTLA4. Vectors coding for the human CTL4-Ig were used for the human B-cell line Raji. Transduction efficiency was over 90% (MOI = 3). For the murine B-cell line A20 and for primary mouse B cells, murine CTLA4 was used. We show that B cells with reduced B7 expression reduce the antigen (Ag) specific TC proliferation in vitro. B cells expressing an ER-directed CTLA4 may reduce Ag-specific immune responses.
Y1  - 2009
UR  - http://informahealthcare.com/loi/aut
U6  - https://doi.org/10.1080/08916930902832470
SN  - 0891-6934
ER  - 
TY  - BOOK
A1  - Gänz, Victoria
A1  - Hinz, Carsten
A1  - Huhn, Nicola
A1  - Klitsch, Constantin
A1  - Löffler, Robert
A1  - Meis, Robin
A1  - Penning, Isabelle
A1  - Richter, Christin
A1  - Schellen, Ricarda
A1  - Schrödter, Ayla
A1  - Schulz, Oliver
A1  - Simon, Veronika
A1  - Trojecka, Anetta
A1  - Verwohlt, Peter
A1  - Vogler, Christin
A1  - Vogt, Birgit
T1  - #Gesellschaftslehre 7/8
BT  - Gesellschaftslehre für die Gesamtschule und Sekundarschule
Y1  - 2022
SN  - 978-3-661-70052-6
VL  - [Schülerband]
PB  - C.C. Buchner
CY  - Bamberg
ER  - 
TY  - BOOK
A1  - Akyazi, Mehmet
A1  - Bergmann, Dana
A1  - Dieterichs, Michael
A1  - Gänz, Victoria
A1  - Hinz, Carsten
A1  - Huhn, Nicola
A1  - Klitsch, Constantin
A1  - Koch, Hayo
A1  - Löffler, Robert
A1  - Meis, Robin
A1  - Penning, Isabelle
A1  - Rasky, Nicolai
A1  - Richter, Christin
A1  - Schellen, Ricarda
A1  - Schrödter, Ayla
A1  - Schulz, Oliver
A1  - Simon, Veronika
A1  - Trojecka, Anetta
A1  - Verwohlt, Peter
A1  - Vogler, Christin
A1  - Vogt, Birgit
T1  - #Gesellschaftslehre 9/10
BT  - Gesellschaftslehre für die Gesamtschule und Sekundarschule
Y1  - 2023
SN  - 978-3-661-70053-3
VL  - [Schülerband]
PB  - C.C. Buchner
CY  - Bamberg
ER  -