TY  - JOUR
A1  - Kadjane, Pascal
A1  - Starck, Matthieu
A1  - Camerel, Franck
A1  - Hill, Diana
A1  - Hildebrandt, Niko
A1  - Ziessel, Raymond
A1  - Charbonnière, Loïc J.
T1  - Divergent approach to a large variety of versatile luminescent lanthanide complexes
N2  - Using a regioselective strategy for nucleophilic aromatic substitution on polyfluoropyridines, a nonacoordinating precursor was designed that is adequately suited for complexation of lanthanide cations. Further functionalizations afforded numerous applications for near-IR emission, two-photon absorption spectroscopy, or the formation of luminescent gels.
Y1  - 2009
UR  - http://pubs.acs.org/journal/inocaj
U6  - https://doi.org/10.1021/Ic9001169
SN  - 0020-1669
ER  - 
TY  - JOUR
A1  - Geißler, Daniel
A1  - Charbonnière, Loïc J.
A1  - Ziessel, Raymond F.
A1  - Löhmannsröben, Hans-Gerd
T1  - Quantum dots as FRET acceptors for highly sensitive multiplexing immunoassays
Y1  - 2009
ER  - 
TY  - JOUR
A1  - Geißler, Daniel
A1  - Charbonnière, Loïc J.
A1  - Ziessel, Raymond F.
A1  - Butlin, Nathaniel G.
A1  - Löhmannsröben, Hans-Gerd
A1  - Hildebrandt, Niko
T1  - Quantum dot biosensors for ultrasensitive multiplexed diagnostics
N2  - Time- and color-resolved detection of Foerster resonance energy transfer (FRET) from luminescent terbium complexes to different semiconductor quantum dots results in a fivefold multiplexed bioassay with sub-picomolar detection limits for all five bioanalytes (see picture). The detection of up to five biomarkers occurs with a sensitivity that is 40-240-fold higher than one of the best-established single-analyte reference assays.
Y1  - 2010
UR  - http://www3.interscience.wiley.com/cgi-bin/jhome/26737/
U6  - https://doi.org/10.1002/anie.200906399
SN  - 1433-7851
ER  - 
TY  - GEN
A1  - Cywiński, Piotr J.
A1  - Nono, Katia Nchimi
A1  - Charbonnière, Loïc J.
A1  - Hammann, Tommy
A1  - Löhmannsröben, Hans-Gerd
T1  - Photophysical evaluation of a new functional terbium complex in FRET-based time-resolved homogenous fluoroassays
N2  - A new functional luminescent lanthanide complex (LLC) has been synthesized with terbium as a central lanthanide ion and biotin as a functional moiety. Unlike in typical lanthanide complexes assembled via carboxylic moieties, in the presented complex, four phosphate groups are chelating the central lanthanide ion. This special chemical assembly enhances the complex stability in phosphate buffers conventionally used in biochemistry. The complex synthesis strategy and photophysical properties are described as well as the performance in time-resolved Förster Resonance Energy Transfer (FRET) assays. In those assays, this biotin-LLC transferred energy either to acceptor organic dyes (Cy5 or AF680) labelled on streptavidin or to quantum dots (QD655 or QD705) surface-functionalised with streptavidins. The permanent spatial donor–acceptor proximity is assured through strong and stable biotin–streptavidin binding. The energy transfer is evidenced from the quenching observed in donor emission and from a decrease in donor luminescence decay, both associated with simultaneous increase in acceptor intensity and in the decay time. The dye-based assays are realised in TRIS and in PBS, whereas QD-based systems are studied in borate buffer. The delayed emission analysis allows for quantifying the recognition process and for auto-fluorescence-free detection, which is particularly relevant for application in bioanalysis. In accordance with Förster theory, Förster-radii (R0) were found to be around 60 Å for organic dyes and around 105 Å for QDs. The FRET efficiency (η) reached 80% and 25% for dye and QD acceptors, respectively. Physical donor–acceptor distances (r) have been determined in the range 45–60 Å for organic dye acceptors, while for acceptor QDs between 120 Å and 145 Å. This newly synthesised biotin-LLC extends the class of highly sensitive analytical tools to be applied in the bioanalytical methods such as time-resolved fluoroimmunoassays (TR-FIA), luminescent imaging and biosensing.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 252 
KW  - acceptors
KW  - bioanalysis
KW  - contrast agents
KW  - europium
KW  - fluoroimmunoassay
KW  - labels
KW  - lanthanide luminescence
KW  - quantum dots
KW  - resonance energy-transfer
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-95390
SP  - 6060
EP  - 6067
ER  -