TY  - JOUR
A1  - Zhou, Ying
A1  - Zhang, Ling
A1  - Gui, Jiadong
A1  - Dong, Fang
A1  - Cheng, Sihua
A1  - Mei, Xin
A1  - Zhang, Linyun
A1  - Li, Yongqing
A1  - Su, Xinguo
A1  - Baldermann, Susanne
A1  - Watanabe, Naoharu
A1  - Yang, Ziyin
T1  - Molecular Cloning and Characterization of a Short-Chain Dehydrogenase Showing Activity with Volatile Compounds Isolated from Camellia sinensis
JF  - Plant molecular biology reporter
N2  - Camellia sinensis synthesizes and emits a large variety of volatile phenylpropanoids and benzenoids (VPB). To investigate the enzymes involved in the formation of these VPB compounds, a new C. sinensis short-chain dehydrogenase/reductase (CsSDR) was isolated, cloned, sequenced, and functionally characterized. The complete open reading frame of CsSDR contains 996 nucleotides with a calculated protein molecular mass of 34.5 kDa. The CsSDR recombinant protein produced in Escherichia coli exhibited dehydrogenase-reductase activity towards several major VPB compounds in C. sinensis flowers with a strong preference for NADP/NADPH co-factors, and showed affinity for (R)/(S)-1-phenylethanol (1PE), phenylacetaldehyde, benzaldehyde, and benzyl alcohol, and no affinity for acetophenone (AP) and 2-phenylethanol. CsSDR showed the highest catalytic efficiency towards (R)/(S)-1PE. Furthermore, the transient expression analysis in Nicotiana benthamiana plants validated that CsSDR could convert 1PE to AP in plants. CsSDR transcript level was not significantly affected by floral development and some jasmonic acid-related environmental stress, and CsSDR transcript accumulation was detected in most floral tissues such as receptacle and anther, which were main storage locations of VPB compounds. Our results indicate that CsSDR is expressed in C. sinensis flowers and is likely to contribute to a number of floral VPB compounds including the 1PE derivative AP.
KW  - Camellia sinensis
KW  - 1-Phenylethanol
KW  - Phenylpropanoids
KW  - Short chain dehydrogenase
KW  - Volatile compound
Y1  - 2015
U6  - https://doi.org/10.1007/s11105-014-0751-z
SN  - 0735-9640
SN  - 1572-9818
VL  - 33
IS  - 2
SP  - 253
EP  - 263
PB  - Springer
CY  - New York
ER  - 
TY  - JOUR
A1  - Zhou, Ying
A1  - Zeng, Lanting
A1  - Fu, Xiumin
A1  - Mei, Xin
A1  - Cheng, Sihua
A1  - Liao, Yinyin
A1  - Deng, Rufang
A1  - Xu, Xinlan
A1  - Jiang, Yueming
A1  - Duan, Xuewu
A1  - Baldermann, Susanne
A1  - Yang, Ziyin
T1  - The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato
JF  - Scientific reports
N2  - The physiological functions of sphingolipids in animals have been intensively studied, while less attention has been paid to their roles in plants. Here, we reveal the involvement of sphingolipid delta8 desaturase (SlSLD) in the chilling resistance of tomato (Solanum lycopersicum cv. Micro-Tom). We used the virus-induced gene silencing (VIGS) approach to knock-down SlSLD expression in tomato leaves, and then evaluated chilling resistance. Changes in leaf cell structure under a chilling treatment were observed by transmission electron microscopy. In control plants, SlSLD was highly expressed in the fruit and leaves in response to a chilling treatment. The degree of chilling damage was greater in SlSLD-silenced plants than in control plants, indicating that SlSLD knock-down significantly reduced the chilling resistance of tomato. Compared with control plants, SlSLD-silenced plants showed higher relative electrolytic leakage and malondialdehyde content, and lower superoxide dismutase and peroxidase activities after a chilling treatment. Chilling severely damaged the chloroplasts in SlSLD-silenced plants, resulting in the disruption of chloroplast membranes, swelling of thylakoids, and reduced granal stacking. Together, these results show that SlSLD is crucial for chilling resistance in tomato.
Y1  - 2016
U6  - https://doi.org/10.1038/srep38742
SN  - 2045-2322
VL  - 6
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - JOUR
A1  - Yang, Ziyin
A1  - Baldermann, Susanne
A1  - Watanabe, Naoharu
T1  - Recent studies of the volatile compounds in tea
JF  - FOOD RESEARCH INTERNATIONAL
N2  - Tea aroma is one of the most important factors affecting the character and quality of tea. Recent advances in methods and instruments for separating and identifying volatile compounds have led to intensive investigations of volatile compounds in tea. These studies have resulted in a number of insightful and useful discoveries. Here we summarize the recent investigations into tea volatile compounds: the volatile compounds in tea products; the metabolic pathways of volatile formation in tea plants and the glycosidically-bound volatile compounds in tea; and the techniques used for studying such compounds. Finally, we discuss practical applications for the improvement of aroma and flavor quality in teas. (C) 2013 Elsevier Ltd. All rights reserved.
KW  - Biosynthesis
KW  - Precursor
KW  - Stress
KW  - Tea
KW  - Volatile
Y1  - 2013
U6  - https://doi.org/10.1016/j.foodres.2013.02.011
SN  - 0963-9969
VL  - 53
IS  - 2
SP  - 585
EP  - 599
PB  - ELSEVIER SCIENCE BV
CY  - AMSTERDAM
ER  - 
TY  - JOUR
A1  - Yamamoto, Masayoshi
A1  - Baldermann, Susanne
A1  - Yoshikawa, Keisuke
A1  - Fujita, Akira
A1  - Mase, Nobuyuki
A1  - Watanabe, Naoharu
T1  - Determination of volatile compounds in four commercial samples of japanese green algae using solid phase microextraction gas chromatography mass spectrometry
JF  - The ScientificWorld journal
N2  - Green algae are of great economic importance. Seaweed is consumed fresh or as seasoning in Japan. The commercial value is determined by quality, color, and flavor and is also strongly influenced by the production area. Our research, based on solid phase microextraction gas chromatography mass spectrometry (SPME-GC-MS), has revealed that volatile compounds differ intensely in the four varieties of commercial green algae. Accordingly, 41 major volatile compounds were identified. Heptadecene was the most abundant compound from Okayama (Ulva prolifera), Tokushima (Ulva prolifera), and Ehime prefecture (Ulva linza). Apocarotenoids, such as ionones, and their derivatives were prominent volatiles in algae from Okayama (Ulva prolifera) and Tokushima prefecture (Ulva prolifera). Volatile, short chained apocarotenoids are among the most potent flavor components and contribute to the flavor of fresh, processed algae, and algae-based products. Benzaldehyde was predominant in seaweed from Shizuoka prefecture (Monostroma nitidum). Multivariant statistical analysis (PCA) enabled simple discrimination of the samples based on their volatile profiles. This work shows the potential of SPME-GC-MS coupled with multivariant analysis to discriminate between samples of different geographical and botanical origins and form the basis for development of authentication methods of green algae products, including seasonings.
Y1  - 2014
U6  - https://doi.org/10.1155/2014/289780
SN  - 1537-744X
PB  - Hindawi Publishing Corp.
CY  - New York
ER  - 
TY  - JOUR
A1  - Yadav, Heena
A1  - Dreher, Dorothée
A1  - Athmer, Benedikt
A1  - Porzel, Andrea
A1  - Gavrin, Aleksandr
A1  - Baldermann, Susanne
A1  - Tissier, Alain
A1  - Hause, Bettina
T1  - Medicago TERPENE SYNTHASE 10 is involved in defense against an oomycete root pathogen
JF  - Plant physiology : an international journal devoted to physiology, biochemistry, cellular and molecular biology, biophysics and environmental biology of plants
N2  - In nature, plants interact with numerous beneficial or pathogenic soil-borne microorganisms. Plants have developed various defense strategies to expel pathogenic microbes, some of which function soon after pathogen infection. We used Medicago truncatula and its oomycete pathogen Aphanomyces euteiches to elucidate early responses of the infected root. A. euteiches causes root rot disease in legumes and is a limiting factor in legume production. Transcript profiling of seedlings and adult plant roots inoculated with A. euteiches zoospores for 2 h revealed specific upregulation of a gene encoding a putative sesquiterpene synthase (M. truncatula TERPENE SYNTHASE 10 [MtTPS10]) in both developmental stages. MtTPS10 was specifically expressed in roots upon oomycete infection. Heterologous expression of MtTPS10 in yeast led to production of a blend of sesquiterpenes and sesquiterpene alcohols, with NMR identifying a major peak corresponding to himalachol. Moreover, plants carrying a tobacco (Nicotiana tabacum) retrotransposon Tnt1 insertion in MtTPS10 lacked the emission of sesquiterpenes upon A. euteiches infection, supporting the assumption that the identified gene encodes a multiproduct sesquiterpene synthase. Mttps10 plants and plants with reduced MtTPS10 transcript levels created by expression of an MtTPS10-artificial microRNA in roots were more susceptible to A. euteiches infection than were the corresponding wild-type plants and roots transformed with the empty vector, respectively. Sesquiterpenes produced by expression of MtTPS10 in yeast also inhibited mycelial growth and A. euteiches zoospore germination. These data suggest that sesquiterpene production in roots by MtTPS10 plays a previously unrecognized role in the defense response of M. truncatula against A. euteiches.
Y1  - 2019
U6  - https://doi.org/10.1104/pp.19.00278
SN  - 0032-0889
SN  - 1532-2548
VL  - 180
IS  - 3
SP  - 1598
EP  - 1613
PB  - American Society of Plant Physiologists
CY  - Rockville
ER  - 
TY  - JOUR
A1  - Witzel, Katja
A1  - Strehmel, Nadine
A1  - Baldermann, Susanne
A1  - Neugart, Susanne
A1  - Becker, Yvonne
A1  - Becker, Matthias
A1  - Berger, Beatrice
A1  - Scheel, Dierk
A1  - Grosch, Rita
A1  - Schreiner, Monika
A1  - Ruppel, Silke
T1  - Arabidopsis thaliana root and root exudate metabolism is altered by the growth-promoting bacterium Kosakonia radicincitans DSM 16656(T)
JF  - Plant and soil
N2  - Plant growth-promoting bacteria (PGPB) affect host physiological processes in various ways. This study aims at elucidating the dependence of bacterial-induced growth promotion on the plant genotype and characterizing plant metabolic adaptations to PGPB. Eighteen Arabidopsis thaliana accessions were inoculated with the PGPB strain Kosakonia radicincitans DSM 16656(T). Colonisation pattern was assessed by enhanced green fluorescent protein (eGFP)-tagged K. radicincitans in three A. thaliana accessions differing in their growth response. Metabolic impact of bacterial colonisation was determined for the best responding accession by profiling distinct classes of plant secondary metabolites and root exudates. Inoculation of 18 A. thaliana accessions resulted in a wide range of growth responses, from repression to enhancement. Testing the bacterial colonisation of three accessions did not reveal a differential pattern. Profiling of plant secondary metabolites showed a differential accumulation of glucosinolates, phenylpropanoids and carotenoids in roots. Analysis of root exudates demonstrated that primary and secondary metabolites were predominantly differentially depleted by bacterial inoculation. The plant genotype controls the bacterial growth promoting traits. Levels of lutein and beta-carotene were elevated in inoculated roots. Supplementing a bacterial suspension with beta-carotene increased bacterial growth, while this was not the case when lutein was applied, indicating that beta-carotene could be a positive regulator of plant growth promotion.
KW  - Arabidopsis
KW  - Carotenoids
KW  - Glucosinolates
KW  - Plant growth promoting bacteria
KW  - Phenylpropanoids
KW  - Root exudates
Y1  - 2017
U6  - https://doi.org/10.1007/s11104-017-3371-1
SN  - 0032-079X
SN  - 1573-5036
VL  - 419
SP  - 557
EP  - 573
PB  - Springer
CY  - Dordrecht
ER  - 
TY  - GEN
A1  - Witzel, Katja
A1  - Neugart, Susanne
A1  - Ruppel, Silke
A1  - Schreiner, Monika
A1  - Wiesner, Melanie
A1  - Baldermann, Susanne
T1  - Recent progress in the use of ‘omics technologies in brassicaceous vegetables
T2  - Frontiers in plant science
N2  - Continuing advances in 'omics methodologies and instrumentation is enhancing the understanding of how plants cope with the dynamic nature of their growing environment. 'Omics platforms have been only recently extended to cover horticultural crop species. Many of the most widely cultivated vegetable crops belong to the genus Brassica: these include plants grown for their root (turnip, rutabaga/swede), their swollen stem base (kohlrabi), their leaves (cabbage, kale, pak choi) and their inflorescence (cauliflower, broccoli). Characterization at the genome, transcript, protein and metabolite levels has illustrated the complexity of the cellular response to a whole series of environmental stresses, including nutrient deficiency, pathogen attack, heavy metal toxicity, cold acclimation, and excessive and sub optimal irradiation. This review covers recent applications of omics technologies to the brassicaceous vegetables, and discusses future scenarios in achieving improvements in crop end-use quality.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 429 
KW  - genomics
KW  - transcriptomics
KW  - metabolomics
KW  - proteomics
KW  - crop
KW  - microbiomics
Y1  - 2018
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-406479
ER  - 
TY  - JOUR
A1  - Witzel, Katja
A1  - Neugart, Susanne
A1  - Ruppel, Silke
A1  - Schreiner, Monika
A1  - Wiesner, Melanie
A1  - Baldermann, Susanne
T1  - Recent progress in the use of 'omics technologies in brassicaceous vegetables
JF  - Frontiers in plant science
N2  - Continuing advances in 'omics methodologies and instrumentation is enhancing the understanding of how plants cope with the dynamic nature of their growing environment. 'Omics platforms have been only recently extended to cover horticultural crop species. Many of the most widely cultivated vegetable crops belong to the genus Brassica: these include plants grown for their root (turnip, rutabaga/swede), their swollen stem base (kohlrabi), their leaves (cabbage, kale, pak choi) and their inflorescence (cauliflower, broccoli). Characterization at the genome, transcript, protein and metabolite levels has illustrated the complexity of the cellular response to a whole series of environmental stresses, including nutrient deficiency, pathogen attack, heavy metal toxicity, cold acclimation, and excessive and sub optimal irradiation. This review covers recent applications of omics technologies to the brassicaceous vegetables, and discusses future scenarios in achieving improvements in crop end-use quality.
KW  - genomics
KW  - transcriptomics
KW  - metabolomics
KW  - proteomics
KW  - crop
KW  - microbiomics
Y1  - 2015
U6  - https://doi.org/10.3389/fpls.2015.00244
SN  - 1664-462X
VL  - 6
PB  - Frontiers Research Foundation
CY  - Lausanne
ER  - 
TY  - JOUR
A1  - Wiesner-Reinhold, Melanie
A1  - Schreiner, Monika
A1  - Baldermann, Susanne
A1  - Schwarz, Dietmar
A1  - Hanschen, Franziska S.
A1  - Kipp, Anna Patricia
A1  - Rowan, Daryl D.
A1  - Bentley-Hewitt, Kerry L.
A1  - McKenzie, Marian J.
T1  - Mechanisms of Selenium Enrichment and Measurement in Brassicaceous Vegetables, and Their Application to Human Health
JF  - Frontiers in plant science
N2  - Selenium (Se) is an essential micronutrient for human health. Se deficiency affects hundreds of millions of people worldwide, particularly in developing countries, and there is increasing awareness that suboptimal supply of Se can also negatively affect human health. Selenium enters the diet primarily through the ingestion of plant and animal products. Although, plants are not dependent on Se they take it up from the soil through the sulphur (S) uptake and assimilation pathways. Therefore, geographic differences in the availability of soil Se and agricultural practices have a profound influence on the Se content of many foods, and there are increasing efforts to biofortify crop plants with Se. Plants from the Brassicales are of particular interest as they accumulate and synthesize Se into forms with additional health benefits, such as methylselenocysteine (MeSeCys). The Brassicaceae are also well-known to produce the glucosinolates; S-containing compounds with demonstrated human health value. Furthermore, the recent discovery of the selenoglucosinolates in the Brassicaceae raises questions regarding their potential bioefficacy. In this review we focus on Se uptake and metabolism in the Brassicaceae in the context of human health, particularly cancer prevention and immunity. We investigate the close relationship between Se and S metabolism in this plant family, with particular emphasis on the selenoglucosinolates, and consider the methodologies available for identifying and quantifying further novel Se-containing compounds in plants. Finally, we summarize the research of multiple groups investigating biofortification of the Brassicaceae and discuss which approaches might be most successful for supplying Se deficient populations in the future.
KW  - Brassica vegetables
KW  - selenium
KW  - biofortification
KW  - glucosinolates
KW  - human health
KW  - immune system
KW  - cancer
KW  - analytical methods
Y1  - 2017
U6  - https://doi.org/10.3389/fpls.2017.01365
SN  - 1664-462X
VL  - 8
PB  - Frontiers Research Foundation
CY  - Lausanne
ER  - 
TY  - JOUR
A1  - Tu, Vo Anh
A1  - Kaga, Atsushi
A1  - Gericke, Karl-Heinz
A1  - Watanabe, Naoharu
A1  - Narumi, Tetsuo
A1  - Toda, Mitsuo
A1  - Brueckner, Bernhard
A1  - Baldermann, Susanne
A1  - Mase, Nobuyuki
T1  - Synthesis and characterization of quantum dot nanoparticles bound to the plant volatile precursor of Hydroxy-apo-10'-carotenal
JF  - The journal of organic chemistry
N2  - This study is focused on the synthesis and characterization of hydroxy-apo-10'-carotenal/quantum dot (QD) conjugates aiming at the in vivo visualization of beta-ionone, a carotenoid-derived volatile compound known for its important contribution to the flavor and aroma of many fruits, vegetables, and plants. The synthesis of nanoparticles bound to plant volatile precursors was achieved via coupling reaction of the QD to C-27-aldehyde which was prepared from alpha-ionone via 12 steps in 2.4% overall yield. The formation of the QD-conjugate was confirmed by measuring its fluorescence spectrum to observe the occurrence of fluorescence resonance energy transfer.
Y1  - 2014
U6  - https://doi.org/10.1021/jo500605c
SN  - 0022-3263
VL  - 79
IS  - 15
SP  - 6808
EP  - 6815
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - JOUR
A1  - Silva, Bibiana
A1  - Oliveira Costa, Ana Carolina
A1  - Tchewonpi, Sorel Sagu
A1  - Bönick, Josephine
A1  - Huschek, Gerd
A1  - Gonzaga, Luciano Valdemiro
A1  - Fett, Roseane
A1  - Baldermann, Susanne
A1  - Rawel, Harshadrai Manilal
T1  - Comparative quantification and differentiation of bracatinga (Mimosa scabrella Bentham) honeydew honey proteins using targeted peptide markers identified by high-resolution mass spectrometry
JF  - Food research international
N2  - Honey traceability is an important topic, especially for honeydew honeys, due to the increased incidence of adulteration. This study aimed to establish specific markers to quantify proteins in honey. A proteomics strategy to identify marker peptides from bracatinga honeydew honey was therefore developed. The proteomics approach was based on initial untargeted identification of honey proteins and peptides by LC-ESI-Triple-TOF-MS/MS, which identified the major royal jelly proteins (MRJP) presence. Afterwards, the peptides were selected by the in silico digestion. The marker peptides were quantified by the developed targeted LC-QqQ-MS/MS method, which provided good linearity and specificity, besides recoveries between 92 and 100% to quantify peptides from bracatinga honeydew honey. The uniqueness and high response in mass spectrometry were backed by further complementary protein analysis (SDS-PAGE). The selected marker peptides EALPHVPIFDR (MRJP 1), ILGANVK (MRJP 2), TFVTIER (MRJP 3), QNIDVVAR (MRJP 4), FINNDYNFNEVNFR (MRJP 5) and LLQPYPDWSWTK (MRJP 7), quantified by LC-QqQ-MS/MS, highlighted that the content of QNIDVVAR from MRJP 4 could be used to differentiate bracatinga honeydew honey from floral honeys (p < 0.05) as a potential marker for its authentication. Finally, principal components analysis highlighted the QNIDVVAR content as a good descriptor of the analyzed bracatinga honeydew honey samples.
KW  - Honeydew honey
KW  - Major royal jelly proteins
KW  - Marker peptides
KW  - High-resolution mass spectrometry
KW  - Principal component analysis
Y1  - 2020
U6  - https://doi.org/10.1016/j.foodres.2020.109991
SN  - 0963-9969
SN  - 1873-7145
VL  - 141
PB  - Elsevier
CY  - New York, NY [u.a.]
ER  - 
TY  - JOUR
A1  - Shi, Jiang
A1  - Xie, Dongchao
A1  - Qi, Dandan
A1  - Peng, Qunhua
A1  - Chen, Zongmao
A1  - Schreiner, Monika
A1  - Lin, Zhi
A1  - Baldermann, Susanne
T1  - Methyl jasmonate-induced changes of flavor profiles during the processing of Green, Oolong, and Black Tea
JF  - Frontiers in plant science
N2  - Tea aroma is one of the most important factors affecting the character and quality of tea. Here we describe the practical application of methyl jasmonate (MeJA) to improve the aroma quality of teas. The changes of selected metabolites during crucial tea processing steps, namely, withering, fixing and rolling, and fermentation, were analyzed. MeJA treatment of tea leaves (12, 24, 48, and 168 h) greatly promotes the aroma quality of green, oolong, and black tea products when comparing with untreated ones (0 h) and as confirmed by sensory evaluation. MeJA modulates the aroma profiles before, during, and after processing. Benzyl alcohol, benzaldehyde, 2-phenylethyl alcohol, phenylacetaldehyde, and trans-2-hexenal increased 1.07- to 3-fold in MeJA-treated fresh leaves and the first two maintained at a higher level in black tea and the last two in green tea. This correlates with a decrease in aromatic amino acids by more than twofold indicating a direct relation to tryptophan- and phenylalanine-derived volatiles. MeJA-treated oolong tea was characterized by a more pleasant aroma. Especially the terpenoids linalool and oxides, geraniol, and carvenol increased by more than twofold.
KW  - methyl jasmonate
KW  - aroma quality
KW  - volatile compounds
KW  - amino acids
KW  - tea processing
Y1  - 2019
U6  - https://doi.org/10.3389/fpls.2019.00781
SN  - 1664-462X
VL  - 10
PB  - Frontiers Research Foundation
CY  - Lausanne
ER  - 
TY  - JOUR
A1  - Schmiedeskamp, Amy
A1  - Schreiner, Monika
A1  - Baldermann, Susanne
T1  - Impact of cultivar selection and thermal processing by air drying, air frying, and deep frying on the carotenoid content and stability and antioxidant capacity in carrots (Daucus carota L.)
JF  - Journal of agricultural and food chemistry : a publication of the American Chemical Society
N2  - Epidemiological data suggest that consuming diets rich in carotenoids can reduce the risk of developing several non-communicable diseases. Thus, we investigated the extent to which carotenoid contents of foods can be increased by the choice of food matrices with naturally high carotenoid contents and thermal processing methods that maintain their stability. For this purpose, carotenoids of 15 carrot (Daucus carota L.) cultivars of different colors were assessed with UHPLC-DAD-ToF-MS. Additionally, the processing effects of air drying, air frying, and deep frying on carotenoid stability were applied. Cultivar selection accounted for up to 12.9-fold differences in total carotenoid content in differently colored carrots and a 2.2-fold difference between orange carrot cultivars. Air frying for 18 and 25 min and deep frying for 10 min led to a significant decrease in total carotenoid contents. TEAC assay of lipophilic extracts showed a correlation between carotenoid content and antioxidant capacity in untreated carrots.
KW  - air-dried
KW  - air-fried
KW  - deep-fried
KW  - domestic cooking
KW  - TEAC
KW  - color
KW  - Daucus
KW  - carota L
Y1  - 2022
U6  - https://doi.org/10.1021/acs.jafc.1c05718
SN  - 0021-8561
SN  - 1520-5118
VL  - 70
IS  - 5
SP  - 1629
EP  - 1639
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - JOUR
A1  - Raila, Jens
A1  - Schweigert, Florian J.
A1  - Stanitznig, A.
A1  - Lambacher, B.
A1  - Franz, S.
A1  - Baldermann, Susanne
A1  - Wittek, T.
T1  - No detectable carotenoid concentrations in serum of llamas and alpacas
JF  - Journal of animal physiology and animal nutrition
N2  - Carotenoids are lipid-soluble pigments and important for a variety of physiological functions. They are major dietary vitamin A precursors and act as lipophilic antioxidants in a variety of tissues and are associated with important health benefits in humans and animals. All animals must acquire carotenoids from their diet, but to our knowledge, there are no studies investigating the intestinal carotenoid absorption and their blood concentrations in New World camelids. The present study aimed to assess the serum concentrations of selected carotenoids in llamas (n=13) and alpacas (n=27). Serum carotenoids as well as retinol (vitamin A) and -tocopherol (vitamin E) were determined by high-performance liquid chromatography coupled with mass spectrometry and these were unable to detect any carotenoids (- and -carotene, - and -cryptoxanthin, lutein, zeaxanthin, lycopene) in the samples. The concentrations of retinol in alpacas (2.89 +/- 1.13mol/l; mean +/- SD) were higher (p=0.024) than those found in llamas (2.05 +/- 0.87mol/l); however, the concentrations of -tocopherol were not significantly (p=0.166) different (llamas: 3.98 +/- 1.83mol/l; alpacas: 4.95 +/- 2.14mol/l). The results show that both llamas and alpacas are not able to absorb intact carotenoids, but efficiently convert provitamin A carotenoids to retinol.
KW  - New World camelids
KW  - carotenoids
KW  - vitamins
Y1  - 2017
U6  - https://doi.org/10.1111/jpn.12638
SN  - 0931-2439
SN  - 1439-0396
VL  - 101
SP  - 629
EP  - 634
PB  - Wiley
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Raffeiner, Margot
A1  - Üstün, Suayib
A1  - Guerra, Tiziana
A1  - Spinti, Daniela
A1  - Fitzner, Maria
A1  - Sonnewald, Sophia
A1  - Baldermann, Susanne
A1  - Börnke, Frederik
T1  - The Xanthomonas type-III effector XopS stabilizes CaWRKY40a to regulate defense responses and stomatal immunity in pepper (Capsicum annuum)
JF  - The plant cell
N2  - As a critical part of plant immunity, cells that are attacked by pathogens undergo rapid transcriptional reprogramming to minimize virulence. Many bacterial phytopathogens use type III effector (T3E) proteins to interfere with plant defense responses, including this transcriptional reprogramming. Here, we show that Xanthomonas outer protein S (XopS), a T3E of Xanthomonas campestris pv. vesicatoria (Xcv), interacts with and inhibits proteasomal degradation of WRKY40, a transcriptional regulator of defense gene expression. Virus-induced gene silencing of WRKY40 in pepper (Capsicum annuum) enhanced plant tolerance to Xcv infection, indicating that WRKY40 represses immunity. Stabilization of WRKY40 by XopS reduces the expression of its targets, which include salicylic acid-responsive genes and the jasmonic acid signaling repressor JAZ8. Xcv bacteria lacking XopS display significantly reduced virulence when surface inoculated onto susceptible pepper leaves. XopS delivery by Xcv, as well as ectopic expression of XopS in Arabidopsis thaliana or Nicotiana benthamiana, prevented stomatal closure in response to bacteria and biotic elicitors. Silencing WRKY40 in pepper or N. benthamiana abolished XopS's ability to prevent stomatal closure. This suggests that XopS interferes with both preinvasion and apoplastic defense by manipulating WRKY40 stability and downstream gene expression, eventually altering phytohormone crosstalk to promote pathogen proliferation.
Y1  - 2022
U6  - https://doi.org/10.1093/plcell/koac032
SN  - 1040-4651
SN  - 1532-298X
VL  - 34
IS  - 5
SP  - 1684
EP  - 1708
PB  - Oxford Univ. Press
CY  - Cary
ER  - 
TY  - JOUR
A1  - Olayide, Priscilla
A1  - Large, Annabel
A1  - Stridh, Linnea
A1  - Rabbi, Ismail
A1  - Baldermann, Susanne
A1  - Stavolone, Livia
A1  - Alexandersson, Erik
T1  - Gene expression and metabolite profiling of thirteen Nigerian cassava landraces to elucidate starch and carotenoid composition
JF  - Agronomy
N2  - The prevalence of vitamin A deficiency in sub-Saharan Africa necessitates effective approaches to improve provitamin A content of major staple crops. Cassava holds much promise for food security in sub-Saharan Africa, but a negative correlation between beta-carotene, a provitamin A carotenoid, and dry matter content has been reported, which poses a challenge to cassava biofortification by conventional breeding. To identify suitable material for genetic transformation in tissue culture with the overall aim to increase beta-carotene and maintain starch content as well as better understand carotenoid composition, root and leaf tissues from thirteen field-grown cassava landraces were analyzed for agronomic traits, carotenoid, chlorophyll, and starch content. The expression of five genes related to carotenoid biosynthesis were determined in selected landraces. Analysis revealed a weak negative correlation between starch and beta-carotene content, whereas there was a strong positive correlation between root yield and many carotenoids including beta-carotene. Carotenoid synthesis genes were expressed in both white and yellow cassava roots, but phytoene synthase 2 (PSY2), lycopene-epsilon-cyclase (LCY epsilon), and beta-carotenoid hydroxylase (CHY beta) expression were generally higher in yellow roots. This study identified lines with reasonably high content of starch and beta-carotene that could be candidates for biofortification by further breeding or plant biotechnological means.
KW  - carotenoid biosynthesis
KW  - ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS)
KW  - provitamin A
KW  - biofortification
Y1  - 2020
U6  - https://doi.org/10.3390/agronomy10030424
SN  - 2073-4395
VL  - 10
IS  - 3
SP  - 1
EP  - 16
PB  - MDPI
CY  - Basel
ER  - 
TY  - GEN
A1  - Olayide, Priscilla
A1  - Large, Annabel
A1  - Stridh, Linnea
A1  - Rabbi, Ismail
A1  - Baldermann, Susanne
A1  - Stavolone, Livia
A1  - Alexandersson, Erik
T1  - Gene expression and metabolite profiling of thirteen Nigerian cassava landraces to elucidate starch and carotenoid composition
T2  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - The prevalence of vitamin A deficiency in sub-Saharan Africa necessitates effective approaches to improve provitamin A content of major staple crops. Cassava holds much promise for food security in sub-Saharan Africa, but a negative correlation between beta-carotene, a provitamin A carotenoid, and dry matter content has been reported, which poses a challenge to cassava biofortification by conventional breeding. To identify suitable material for genetic transformation in tissue culture with the overall aim to increase beta-carotene and maintain starch content as well as better understand carotenoid composition, root and leaf tissues from thirteen field-grown cassava landraces were analyzed for agronomic traits, carotenoid, chlorophyll, and starch content. The expression of five genes related to carotenoid biosynthesis were determined in selected landraces. Analysis revealed a weak negative correlation between starch and beta-carotene content, whereas there was a strong positive correlation between root yield and many carotenoids including beta-carotene. Carotenoid synthesis genes were expressed in both white and yellow cassava roots, but phytoene synthase 2 (PSY2), lycopene-epsilon-cyclase (LCY epsilon), and beta-carotenoid hydroxylase (CHY beta) expression were generally higher in yellow roots. This study identified lines with reasonably high content of starch and beta-carotene that could be candidates for biofortification by further breeding or plant biotechnological means.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1415 
KW  - carotenoid biosynthesis
KW  - ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS)
KW  - provitamin A
KW  - biofortification
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-517834
SN  - 1866-8372
IS  - 3
ER  - 
TY  - JOUR
A1  - Odongo, Grace Akinyi
A1  - Schlotz, Nina
A1  - Baldermann, Susanne
A1  - Neugart, Susanne
A1  - Ngwene, Benard
A1  - Schreiner, Monika
A1  - Lamy, Evelyn
T1  - Effects of Amaranthus cruentus L. on aflatoxin B1- and oxidative stress-induced DNA damage in human liver (HepG2) cells
JF  - Food bioscience
N2  - Amaranth is presently an underutilized crop despite its high content of micronutrients/bioactive phytochemicals and its capacity to thrive in harsh environmental condition. The present study aimed at determining the health benefits of Amaranthus cruentus L. in terms of protection against DNA damage induced by the mycotoxin aflatoxin B1 (AFB1) and oxidative stress using comet assay. The antioxidant potential was further investigated using electron paramagnetic resonance spectroscopy (EPR) and an ARE/Nrf2 reporter gene assay in vitro in a human liver model using the HepG2 cell line. Ethanolic extracts from fresh leaves grown under controlled conditions were used and additionally analyzed for their phytochemical content using liquid chromatography-mass spectrometry (LC-MS). The extracts inhibited both AFB1- and oxidative stress-induced DNA damage in a concentration dependent way with a maximum effect of 57% and 81%, respectively. Oxidative stress triggered using ferrous sulfate was blocked by up to 38% (EPR); the potential to induce antioxidant enzymes using ARE/Nrf2-mediated gene expression was also confirmed. Based on these in vitro findings, further studies on the health-protecting effects of A. cruentus are encouraged to fully explore its health promoting potential and provide the scientific basis for encouraging its cultivation and consumption.
KW  - African indigenous vegetables
KW  - Aflatoxin B1
KW  - Amaranthaceae
KW  - Amaranth
KW  - Aspergillus
KW  - Cancer prevention
Y1  - 2018
U6  - https://doi.org/10.1016/j.fbio.2018.09.006
SN  - 2212-4292
SN  - 2212-4306
VL  - 26
SP  - 42
EP  - 48
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - GEN
A1  - Odongo, Grace Akinyi
A1  - Schlotz, Nina
A1  - Baldermann, Susanne
A1  - Neugart, Susanne
A1  - Huyskens-Keil, Susanne
A1  - Ngwene, Benard
A1  - Trierweiler, Bernhard
A1  - Schreiner, Monika
A1  - Lamy, Evelyn
T1  - African nightshade (Solanum scabrum Mill.)
BT  - impact of cultivation and plant processing on its health promoting potential as determined in a human liver cell model
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Plant cultivation and processing may impact nutrient and phytochemical content of vegetables. The present study aimed at determining the influence of cultivation and processing on the health promoting capacity of African nightshade (Solanum scabrum Mill.) leaves, an indigenous vegetable, rich in nutrients and phytochemicals. Anti-genotoxicity against the human liver carcinogen aflatoxin B1 (AFB1) as determined by the comet assay and radical oxygen species (ROS) scavenging capacity of ethanolic and aqueous extracts were investigated in human derived liver (HepG2) cells. ROS scavenging activity was assessed using electron paramagnetic spin resonance and quantification of ARE/Nrf2 mediated gene expression. The cultivation was done under different environmental conditions. The processing included fermentation and cooking; postharvest ultraviolet irradiation (UV-C) treatment was also investigated. Overall, S. scabrum extracts showed strong health promoting potential, the highest potential was observed with the fermented extract, which showed a 60% reduction of AFB1 induced DNA damage and a 38% reduction in FeSO4 induced oxidative stress. The content of total polyphenols, carotenoids and chlorophylls was indeed affected by cultivation and processing. Based on the present in vitro findings consumption of S. scabrum leaves could be further encouraged, preferentially after cooking or fermentation of the plant.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1133 
KW  - aflatoxin B1
KW  - African indigenous vegetables
KW  - anti-genotoxicity
KW  - anti-oxidant activity
KW  - cancer chemoprevention
KW  - Solanaceae
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-459114
SN  - 1866-8372
IS  - 1133
ER  - 
TY  - JOUR
A1  - Odongo, Grace Akinyi
A1  - Schlotz, Nina
A1  - Baldermann, Susanne
A1  - Neugart, Susanne
A1  - Huyskens-Keil, Susanne
A1  - Ngwene, Benard
A1  - Trierweiler, Bernhard
A1  - Schreiner, Monika
A1  - Lamy, Evelyn
T1  - African Nightshade (Solanum scabrum Mill.)
BT  - Impact of Cultivation and Plant Processing on Its Health Promoting Potential as Determined in a Human Liver Cell Model
JF  - Nutrients
N2  - Plant cultivation and processing may impact nutrient and phytochemical content of vegetables. The present study aimed at determining the influence of cultivation and processing on the health promoting capacity of African nightshade (Solanum scabrum Mill.) leaves, an indigenous vegetable, rich in nutrients and phytochemicals. Anti-genotoxicity against the human liver carcinogen aflatoxin B1 (AFB(1)) as determined by the comet assay and radical oxygen species (ROS) scavenging capacity of ethanolic and aqueous extracts were investigated in human derived liver (HepG2) cells. ROS scavenging activity was assessed using electron paramagnetic spin resonance and quantification of ARE/Nrf2 mediated gene expression. The cultivation was done under different environmental conditions. The processing included fermentation and cooking; postharvest ultraviolet irradiation (UV-C) treatment was also investigated. Overall, S. scabrum extracts showed strong health promoting potential, the highest potential was observed with the fermented extract, which showed a 60% reduction of AFB(1) induced DNA damage and a 38% reduction in FeSO4 induced oxidative stress. The content of total polyphenols, carotenoids and chlorophylls was indeed affected by cultivation and processing. Based on the present in vitro findings consumption of S. scabrum leaves could be further encouraged, preferentially after cooking or fermentation of the plant.
KW  - aflatoxin B1
KW  - African indigenous vegetables
KW  - anti-genotoxicity
KW  - anti-oxidant activity
KW  - cancer chemoprevention
KW  - Solanaceae
Y1  - 2018
U6  - https://doi.org/10.3390/nu10101532
SN  - 2072-6643
VL  - 10
IS  - 10
PB  - MDPI
CY  - Basel
ER  -