TY  - JOUR
A1  - Whitcomb, Sarah J.
A1  - Nguyen, Huu Cuong
A1  - Brückner, Franziska
A1  - Hesse, Holger
A1  - Hoefgen, Rainer
T1  - CYSTATHIONINE GAMMA-SYNTHASE activity in rice is developmentally regulated and strongly correlated with sulfate
JF  - Plant science : an international journal of experimental plant biology
N2  - An important goal of rice cultivar development is improvement of protein quality, especially with respect to essential amino acids such as methionine. With the goal of increasing seed methionine content, we generated Oryza sativa ssp. japonica cv. Taipei 309 transgenic lines expressing a feedback-desensitized CYSTATHIONINE GAMMA-SYNTHASE from Arabidopsis thaliana (AtD-CGS) under the control of the maize ubiquitin promoter. Despite persistently elevated cystathionine gamma-synthase (CGS) activity in the AtD-CGS transgenic lines relative to untransformed Taipei, sulfate was the only sulfur-containing compound found to be elevated throughout vegetative development. Accumulation of methionine and other sulfur-containing metabolites was limited to the leaves of young plants. Sulfate concentration was found to strongly and positively correlate with CGS activity across vegetative development, irrespective of whether the activity was provided by the endogenous rice CGS or by a combination of endogenous and AtD-CGS. Conversely, the concentrations of glutathione, valine, and leucine were clearly negatively correlated with CGS activity in the same tissues. We also observed a strong decrease in CGS activity in both untransformed Taipei and the AtD-CGS transgenic lines as the plants approached heading stage. The mechanism for this downregulation is currently unknown and of potential importance for efforts to increase methionine content in rice.
KW  - Aromatic amino acids
KW  - AtD-CGS
KW  - Branched chain amino acids
KW  - CYSTATHIONINE GAMMA-SYNTHASE
KW  - Glutathione
KW  - Oryza sativa ssp japonica cv. taipei 309
KW  - Sulfate
Y1  - 2018
U6  - https://doi.org/10.1016/j.plantsci.2018.02.016
SN  - 0168-9452
VL  - 270
SP  - 234
EP  - 244
PB  - Elsevier
CY  - Clare
ER  - 
TY  - JOUR
A1  - Ahlberg, Sebastian
A1  - Rancan, Fiorenza
A1  - Epple, Matthias
A1  - Loza, Kateryna
A1  - Höppe, David
A1  - Lademann, Jürgen
A1  - Vogt, Annika
A1  - Kleuser, Burkhard
A1  - Gerecke, Christian
A1  - Meinke, Martina C.
T1  - Comparison of different methods to study effects of silver nanoparticles on the pro- and antioxidant status of human keratinocytes and fibroblasts
JF  - Methods : focusing on rapidly developing techniques
KW  - Oxidative stress
KW  - Dichlorofluorescein assay
KW  - Electron paramagnetic resonance spectroscopy
KW  - HaCaT cells
KW  - Glutathione
KW  - Free radicals
Y1  - 2016
U6  - https://doi.org/10.1016/j.ymeth.2016.05.015
SN  - 1046-2023
SN  - 1095-9130
VL  - 109
SP  - 55
EP  - 63
PB  - Elsevier
CY  - San Diego
ER  -