TY  - JOUR
A1  - Huschek, Gerd
A1  - Bönick, Josephine
A1  - Merkel, Dietrich
A1  - Huschek, Doreen
A1  - Rawel, Harshadrai Manilal
T1  - Authentication of leguminous-based products by targeted biomarkers using high resolution time of flight mass spectrometry
JF  - LWT - food science and technology : an official journal of the Swiss Society of Food Science and Technology (SGLWT/SOSSTA) and the International Union of Food Science and Technology (IUFoST)
N2  - A growing number of health-conscious individuals supplements their diet with protein-rich plant-based products to reduce their meat consumption. Analytical methods are needed to authenticate these new vegetarian products not only for the correct labelling of ingredients according to European legislation but also to discourage food fraud. This paper presents new biomarkers for a targeted proteomics LC-MS/MS work-flow that can simultaneously prove the presence/absence of garden pea, a protein-rich legume, meat and honey and quantify their content in processed vegan food. We show a novel rapid strategy to identify biomarkers for species authentication and the steps for the multi-parameter LC-MS/MS method validation and quantification. A high resolution triple time of flight mass spectrometer (HRMS) with SWATH Acquisition was used for the rapid discovery of all measurable trypsin-digested proteins in the individual ingredients. From these proteins, species-selective biomarkers were identified with BLAST and Skyline. Vicilin and convicilin (UniProt: D3VND9, Q9M3X6) allow pea authentication with regard to other legume species. Myostatin (UniProt: 018831) is a single biomarker for all meat types. For honey, we identified three selective proteins (UniProt: C6K481, C6K482, Q3L6329). The final LC-MS/MS method can identity and quantify these markers simultaneously. Quantification occurs via external matrix calibration.
KW  - Vegan
KW  - Food authentication
KW  - Legume
KW  - Honey
KW  - Meat peptide biomarker
KW  - MS quantification of leguminous additives
KW  - Food labelling
Y1  - 2018
U6  - https://doi.org/10.1016/j.lwt.2017.12.034
SN  - 0023-6438
SN  - 1096-1127
VL  - 90
SP  - 164
EP  - 171
PB  - Elsevier
CY  - Amsterdam
ER  -