TY  - JOUR
A1  - Schweigel, Ulrike
A1  - Batsios, Petros
A1  - Müller-Taubenberger, Annette
A1  - Gräf, Ralph
A1  - Grafe, Marianne
T1  - Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis
JF  - Nucleus
N2  - Dictyostelium amoebae perform a semi-closed mitosis, in which the nuclear envelope is fenestrated at the insertion sites of the mitotic centrosomes and around the central spindle during karyokinesis. During late telophase the centrosome relocates to the cytoplasmic side of the nucleus, the central spindle disassembles and the nuclear fenestrae become closed. Our data indicate that Dictyostelium spastin (DdSpastin) is a microtubule-binding and severing type I membrane protein that plays a role in this process. Its mitotic localization is in agreement with a requirement for the removal of microtubules that would hinder closure of the fenestrae. Furthermore, DdSpastin interacts with the HeH/ LEM-family protein Src1 in BioID analyses as well as the inner nuclear membrane protein Sun1, and shows subcellular co-localizations with Src1, Sun1, the ESCRT component CHMP7 and the IST1-like protein filactin, suggesting that the principal pathway of mitotic nuclear envelope remodeling is conserved between animals and Dictyostelium amoebae.
KW  - Spastin
KW  - LEM-domain
KW  - ESCRT
KW  - sun1
KW  - dictyostelium
KW  - nuclear envelope
KW  - mitosis
Y1  - 2022
U6  - https://doi.org/10.1080/19491034.2022.2047289
SN  - 1949-1034
SN  - 1949-1042
VL  - 13
IS  - 1
SP  - 144
EP  - 154
PB  - Taylor & Francis Group
CY  - Philadelphia
ER  - 
TY  - JOUR
A1  - Batsios, Petros
A1  - Peter, Tatjana
A1  - Baumann, Otto
A1  - Stick, Reimer
A1  - Meyer, Irene
A1  - Gräf, Ralph
T1  - A lamin in lower eukaryotes?
JF  - Nucleus
N2  - Lamins are the major components of the nuclear lamina and serve not only as a mechanical support, but are also involved in chromatin organization, epigenetic regulation, transcription and mitotic events. Despite these universal tasks, lamins have so far been found only in metazoans. Yet, recently we have identified Dictyostelium NE81 as the first lamin-like protein in a lower eukaryote. Based on the current knowledge, we draw a model for nuclear envelope organization in Dictyostelium in this Extra View and we review the experimental data that justified this classification. Furthermore we provide unpublished data underscoring the requirement of posttranslational CaaX-box processing for proper protein localization at the nuclear envelope. Sequence comparison of NE81 sequences from four Dictyostelia with bona fide lamins illustrates the evolutional relationship between these proteins. Under certain conditions these usually unicellular social amoebae congregate to form a multicellular body. We propose that the evolution of the lamin-like NE81 went along with the invention of multicellularity.
KW  - dictyostelium
KW  - lamin
KW  - intermediate filament
KW  - centrosome
KW  - nucleus
Y1  - 2012
U6  - https://doi.org/10.4161/nucl.20149
SN  - 1949-1034
VL  - 3
IS  - 3
SP  - 237
EP  - 243
PB  - Landes Bioscience
CY  - Austin
ER  -