TY  - JOUR
A1  - Laux, Eva-Maria
A1  - Bier, Frank Fabian
A1  - Hölzel, Ralph
T1  - Dielectrophoretic Stretching of DNA
JF  - DNA Nanotechnology
N2  - The spatial control of DNA and of self-assembled DNA constructs is a prerequisite for the preparation of DNA-based nanostructures and microstructures and a useful tool for studies on single DNA molecules. Here we describe a protocol for the accumulation of dissolved lambda-DNA molecules between planar microelectrodes by the action of inhomogeneous radiofrequency electric fields. The resulting AC electrokinetic forces stretch the DNA molecules and align them parallel to the electric field. The electrode preparation from off-the-shelf electronic components is explained, and a detailed description of the electronic setup is given. The experimental procedure is controlled in real-time by fluorescence microscopy.
KW  - Alignment
KW  - Dielectrophoresis
KW  - DNA
KW  - Electrokinetics
KW  - Interdigitated electrodes
KW  - Stretching
Y1  - 2018
SN  - 978-1-4939-8582-1
SN  - 978-1-4939-8581-4
U6  - https://doi.org/10.1007/978-1-4939-8582-1_14
SN  - 1064-3745
SN  - 1940-6029
SP  - 199
EP  - 208
PB  - Humana Press Inc.
CY  - New York
ET  - 2
ER  - 
TY  - JOUR
A1  - Laux, Eva-Maria
A1  - Wenger, Christian
A1  - Bier, Frank Fabian
A1  - Hoelzel, Ralph
T1  - AC electrokinetic immobilization of organic dye molecules
JF  - Analytical and bioanalytical chemistry : a merger of Fresenius' journal of analytical chemistry and Analusis
N2  - The application of inhomogeneous AC electric fields for molecular immobilization is a very fast and simple method that does not require any adaptions to the molecule's functional groups or charges. Here, the method is applied to a completely new category of molecules: small organic fluorescence dyes, whose dimensions amount to only 1 nm or even less. The presented setup and the electric field parameters used allow immobilization of dye molecules on the whole electrode surface as opposed to pure dielectrophoretic applications, where molecules are attracted only to regions of high electric field gradients, i.e., to the electrode tips and edges. In addition to dielectrophoresis and AC electrokinetic flow, molecular scale interactions and electrophoresis at short time scales are discussed as further mechanisms leading to migration and immobilization of the molecules.
KW  - AC electrokinetics
KW  - AC electrophoresis
KW  - Molecular dielectrophoresis
KW  - Interdigitated electrodes
KW  - Organic dyes
Y1  - 2020
U6  - https://doi.org/10.1007/s00216-020-02480-4
SN  - 1618-2642
SN  - 1618-2650
VL  - 412
IS  - 16
SP  - 3859
EP  - 3870
PB  - Springer
CY  - Berlin
ER  -