TY - JOUR A1 - Velk, Natalia A1 - Uhlig, Katja A1 - Vikulina, Anna A1 - Duschl, Claus A1 - Volodkin, Dmitry T1 - Mobility of lysozyme in poly(L-lysine)/hyaluronic acid multilayer films JF - Colloids and surfaces : an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin ; B, Biointerfaces N2 - The spatial and temporal control over presentation of protein-based biomolecules such as growth factors and hormones is crucial for in vitro applications to mimic the complex in vivo environment. We investigated the interaction of a model protein lysozyme (Lys) with poly(L-lysine)/hyaluronic acid (PLL/HA) multilayer films. We focused on Lys diffusion as well as adsorption and retention within the film as a function of the film deposition conditions and post-treatment. Additionally, an effect of Lys concentration on its mobility was probed. A combination of confocal fluorescence microscopy, fluorescence recovery after photobleaching, and microfluidics was employed for this investigation. Our main finding is that adsorption of PLL and HA after protein loading induces acceleration and reduction of Lys mobility, respectively. These results suggest that a charge balance in the film to a high extent governs the protein-film interaction. We believe that control over protein mobility is a key to reach the full potential of the PLL/HA films as reservoirs for biomolecules depending on the application demand. (C) 2016 The Authors. Published by Elsevier B.V. KW - Layer-by-layer KW - Protein KW - Diffusion KW - Release KW - FRAP Y1 - 2016 U6 - https://doi.org/10.1016/j.colsurfb.2016.07.055 SN - 0927-7765 SN - 1873-4367 VL - 147 SP - 343 EP - 350 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Fernandez, Ricardo A1 - Bruno, Giovanni A1 - Garces, Gerardo A1 - Nieto-Luis, H. A1 - Gonzalez-Doncel, Gaspar T1 - Fractional brownian motion of dislocations during creep deformation of metals JF - Materials science & engineering. A, Structural materials N2 - The present work offers an explanation on how the long-range interaction of dislocations influences their movement, and therefore the strain, during creep of metals. It is proposed that collective motion of dislocations can be described as a fractional Brownian motion. This explains the noisy appearance of the creep strain signal as a function of time. Such signal is split into a deterministic and a stochastic part. These terms can be related to two kinds of dislocation motions: individual and collective, respectively. The description is consistent with the fractal nature of strain-induced dislocation structures predicated in previous works. Moreover, it encompasses the evolution of the strain rate during all stages of creep, including the tertiary one. Creep data from Al99.8% and Al3.85%Mg tested at different temperatures and stresses are used to validate the proposed ideas: it is found that different creep stages present different diffusion characters, and therefore different dislocation motion character. KW - Creep KW - Aluminum alloys KW - Dislocation motion KW - Diffusion KW - Fractal KW - structures Y1 - 2020 U6 - https://doi.org/10.1016/j.msea.2020.140013 SN - 0921-5093 SN - 1873-4936 VL - 796 PB - Elsevier CY - Lausanne ER - TY - JOUR A1 - Hannigan, Sara A1 - Nendel, Claas A1 - Krull, Marcos T1 - Effects of temperature on the movement and feeding behaviour of the large lupine beetle, Sitona gressorius JF - Journal of pest science N2 - Even though the effects of insect pests on global agricultural productivity are well recognised, little is known about movement and dispersal of many species, especially in the context of global warming. This work evaluates how temperature and light conditions affect different movement metrics and the feeding rate of the large lupine beetle, an agricultural pest responsible for widespread damage in leguminous crops. By using video recordings, the movement of 384 beetles was digitally analysed under six different temperatures and light conditions in the laboratory. Bayesian linear mixed-effect models were used to analyse the data. Furthermore, the effects of temperature on the daily diffusion coefficient of beetles were estimated by using hidden Markov models and random walk simulations. Results of this work show that temperature, light conditions, and beetles' weight were the main factors affecting the flight probability, displacement, time being active and the speed of beetles. Significant variations were also observed in all evaluated metrics. On average, beetles exposed to light conditions and higher temperatures had higher mean speed and flight probability. However, beetles tended to stay more active at higher temperatures and less active at intermediate temperatures, around 20 degrees C. Therefore, both the diffusion coefficient and displacement of beetles were lower at intermediate temperatures. These results show that the movement behaviour and feeding rates of beetles can present different relationships in the function of temperature. It also shows that using a single diffusion coefficient for insects in spatially explicit models may lead to over- or underestimation of pest spread. KW - Agricultural pests KW - Diffusion KW - Hidden Markov models KW - Movement ecology Y1 - 2022 U6 - https://doi.org/10.1007/s10340-022-01510-7 SN - 1612-4758 SN - 1612-4766 SP - 389 EP - 402 PB - Springer CY - Heidelberg ER -