TY - JOUR A1 - Nagel, Birgit A1 - Warsinke, Axel T1 - Towards separation-free electrochemical affinity sensors by using antibodies, aptamers and molecularly imprinted polymers : a review Y1 - 2006 U6 - https://doi.org/10.1080/00032710600853903 ER - TY - JOUR A1 - Huang, T. A1 - Warsinke, Axel A1 - Kuwana, T. A1 - Scheller, Frieder W. T1 - The determination of L-phenylalanine based on a novel NADH-detecting biosensor Y1 - 1998 ER - TY - JOUR A1 - Grießner, Matthias A1 - Hartig, Dave A1 - Christmann, Alexander A1 - Ehrentreich-Förster, Eva A1 - Warsinke, Axel A1 - Bier, Frank Fabian T1 - Surface regeneration of microfluidic microarray printheads through plasma techniques N2 - This work describes a method for surface regeneration of microfluidic microarray printheads through plasma techniques. Modification procedures were chosen in a way to obtain high reproducibility with a minimum of time consumption. The idea behind this is a complete regeneration of a microarray printhead before or after usage to achieve best printing results over a typical print job. A sequence of low-pressure oxygen-plasma and plasma polymerization with hexamethyldisiloxane (HMDSO) was used to regenerate printheads. Proof of the concept is given through quality control performed with a spotter implemented CCD camera, contact angle measurements and a typical hybridization experiment. Stable printing results were obtained over 3000 activations showing that the presented method is suitable for treatment of microarray printheads. Y1 - 2010 UR - http://iopscience.iop.org/0960-1317/ U6 - https://doi.org/10.1088/0960-1317/20/3/037002 SN - 0960-1317 ER - TY - JOUR A1 - Halámek, Jan A1 - Wollenberger, Ursula A1 - Stöcklein, Walter F. M. A1 - Warsinke, Axel A1 - Scheller, Frieder W. T1 - Signal amplification in immunoassays using labeling via boronic acid binding to the sugar moiety of immunoglobulin G : proof of concept for glycated hemoglobin N2 - A novel electrochemical immunoassay based on the multiple affinity labeling of the indicator antibody with an electro-active tag is presented. The concept is illustrated for the determination of the glycated hemoglobin HbA1c in hemoglobin samples. Hemoglobin is adsorbed to the surfactant-modified surface of a piezoelectric quartz crystal. Whereas the quartz crystal nanobalance is used to validate the total Hb binding, the HbA1c on the sensor surface is recognized by an antibody and quantified electrochemically after the sugar moieties of the antibody have been labeled in-situ with ferroceneboronic acid. The sensitivity of this sensor is about threefold higher than the sensitivity of a hemoglobin sensor, where the ferroceneboronic acid is bound directly to HbA1c. Y1 - 2007 UR - http://www.informaworld.com/openurl?genre=journal&issn=0003-2719 U6 - https://doi.org/10.1080/00032710701327096 SN - 0003-2719 ER - TY - JOUR A1 - Stöcklein, Walter F. M. A1 - Rohde, M. A1 - Scharte, Gudrun A1 - Behrsing, Olaf A1 - Warsinke, Axel A1 - Micheel, Burkhard A1 - Scheller, Frieder W. T1 - Sensitive detection of triazine and phenylurea pesticides in pure organic solvent by enzyme linked immunsorbent assay (ELISA): stabilities, solubilities and sensitives Y1 - 2000 ER - TY - JOUR A1 - Scheller, Frieder W. A1 - Wollenberger, Ursula A1 - Warsinke, Axel A1 - Lisdat, Fred T1 - Research and development in biosensors Y1 - 2001 ER - TY - JOUR A1 - Rohde, M. A1 - Schenk, Jörg A. A1 - Heymann, Stephan A1 - Behrsing, Olaf A1 - Scharte, Gudrun A1 - Kempter, Gerhard A1 - Woller, Jochen A1 - Höhne, Wolfgang A1 - Warsinke, Axel A1 - Micheel, Burkhard T1 - Production and characterization of monoclonal antibodeis against urea derivatives Y1 - 1998 ER - TY - JOUR A1 - Warsinke, Axel T1 - Point-of-care testing of proteins N2 - Point-of-care testing (POCT) is a fast developing area in clinical diagnostics that is considered to be one of the main driving forces for the future in vitro diagnostic market. POCT means decentralized testing at the site of patient care. The most important POCT devices are handheld blood glucose sensors. In some of these sensors, after the application of less than 1 A mu l whole blood, the results are displayed in less than 10 s. For protein determination, the most commonly used devices are based on lateral flow technology. Although these devices are convenient to use, the results are often only qualitative or semiquantitative. The review will illuminate some of the current methods employed in POCT for proteins and will discuss the outlook for techniques (e.g., electrochemical immunosensors) that could have a great impact on future POCT of proteins. Y1 - 2009 UR - http://www.springerlink.com/content/100417 U6 - https://doi.org/10.1007/s00216-008-2572-0 SN - 1618-2642 ER - TY - JOUR A1 - Stöcklein, Walter F. M. A1 - Warsinke, Axel A1 - Scheller, Frieder W. T1 - Organic solvent modified enzyme-liked immunoassay for the detection of triazine herbicides Y1 - 1997 ER - TY - JOUR A1 - Gajovic, Nenad A1 - Binyamin, Gary A1 - Warsinke, Axel A1 - Scheller, Frieder W. A1 - Heller, A. T1 - Operation of a miniature redox hydrogel-based pyruvate sensor in undiluted deoxygenated calf serum Y1 - 2000 ER - TY - JOUR A1 - Stöllner, Daniela A1 - Stöcklein, Walter F. M. A1 - Scheller, Frieder W. A1 - Warsinke, Axel T1 - Membrane-immobilized haptoglobin as affinity matrix for a hemoglobin-A1c-immunosensor Y1 - 2002 ER - TY - JOUR A1 - Guha, S. A1 - Warsinke, Axel A1 - Tientcheu, Charles Merlin A1 - Schmalz, K. A1 - Meliani, C. A1 - Wenger, Ch. T1 - Label free sensing of creatinine using a 6 GHz CMOS near-field dielectric immunosensor JF - The analyst : the analytical journal of the Royal Society of Chemistry N2 - In this work we present a CMOS high frequency direct immunosensor operating at 6 GHz (C-band) for label free determination of creatinine. The sensor is fabricated in standard 0.13 mu m SiGe:C BiCMOS process. The report also demonstrates the ability to immobilize creatinine molecules on a Si3N4 passivation layer of the standard BiCMOS/CMOS process, therefore, evading any further need of cumbersome post processing of the fabricated sensor chip. The sensor is based on capacitive detection of the amount of non-creatinine bound antibodies binding to an immobilized creatinine layer on the passivated sensor. The chip bound antibody amount in turn corresponds indirectly to the creatinine concentration used in the incubation phase. The determination of creatinine in the concentration range of 0.88-880 mu M is successfully demonstrated in this work. A sensitivity of 35 MHz/10 fold increase in creatinine concentration (during incubation) at the centre frequency of 6 GHz is gained by the immunosensor. The results are compared with a standard optical measurement technique and the dynamic range and sensitivity is of the order of the established optical indication technique. The C-band immunosensor chip comprising an area of 0.3 mm(2) reduces the sensing area considerably, therefore, requiring a sample volume as low as 2 mu l. The small analyte sample volume and label free approach also reduce the experimental costs in addition to the low fabrication costs offered by the batch fabrication technique of CMOS/BiCMOS process. Y1 - 2015 U6 - https://doi.org/10.1039/c4an02194k SN - 0003-2654 SN - 1364-5528 VL - 140 IS - 9 SP - 3019 EP - 3027 PB - Royal Society of Chemistry CY - Cambridge ER - TY - JOUR A1 - Scheller, Frieder W. A1 - Bauer, Christian G. A1 - Makower, Alexander A1 - Wollenberger, Ursula A1 - Warsinke, Axel A1 - Bier, Frank Fabian T1 - Immunoassays using enzymatic amplification electrodes Y1 - 2002 SN - 0-7484-0791-X ER - TY - JOUR A1 - Sellrie, Frank A1 - Warsinke, Axel A1 - Micheel, Burkhard T1 - Homogeneous indirect fluorescence quenching immunoassay for the determination of low molecular weight substances JF - Analytical & bioanalytical chemistry N2 - This paper describes the principle of a homogeneous indirect fluorescence quenching immunoassay that uses monoclonal antibodies. It is a carrier-free assay system that is performed completely in solution. The assay system was established for the determination of a low molecular weight substance (hapten), the herbicide diuron, used as a model analyte. A fluorescein-monuron conjugate together with a fluorescence-quenching monoclonal anti-fluorescein antibody and an anti-analyte antibody (here an anti-diuron/monuron monoclonal antibody) were used as central components of the assay. The fluorescein-monuron conjugate can be bound either by the anti-fluorescein monoclonal antibody or by the anti-diuron/ monuron monoclonal antibody. Due to steric hindrance, binding of both antibodies to the conjugate was not possible at the same time. By selecting the antibody concentrations appropriately, a dynamic equilibrium can be established that permits the preferential binding of the anti-diuron/monuron antibody to the conjugate, which allows the fluorescein in the conjugate to fluoresce. This equilibrium can be easily altered by adding free analyte (diuron), which competes with the conjugate to bind to the anti-diuron/monuron antibody. A reduction of anti-diuron/monuron antibody binding to the conjugate results in an increase in the binding of the anti-fluorescein antibody, which leads to a decrease in the fluorescence of the conjugate. The fluorescence is therefore a direct indicator of the state of equilibrium of the system and thus also the presence of free unconjugated analyte. The determination of an analyte based on this test principle does not require any washing steps. After the test components are mixed, the dynamic equilibrium is rapidly reached and the results can be obtained in less than 5 min by measuring the fluorescence of the fluorescein. We used this test principle for the determination of diuron, which was demonstrated for concentrations of approximately 5 nM. Y1 - 2008 UR - http://www.springerlink.com/content/n7227875454216v7/ VL - 386 IS - 2 SP - 206 EP - 210 ER - TY - JOUR A1 - Kleinjung, Frank A1 - Beier, Frank F. A1 - Warsinke, Axel A1 - Scheller, Frieder W. T1 - Fibre-optic genosensor for specific determination of femtomolar DNA oligomers Y1 - 1997 ER - TY - JOUR A1 - Stöcklein, Walter F. M. A1 - Behrsing, Olaf A1 - Scharte, Gudrun A1 - Micheel, Burkhard A1 - Benkert, Alexander A1 - Schössler, W. A1 - Warsinke, Axel A1 - Scheller, Frieder W. T1 - Enzyme kinetic assays with surface plasmon resonance (BIAcore) based on competition between enzyme and creatinine antibody Y1 - 2000 ER - TY - JOUR A1 - Warsinke, Axel A1 - Benkert, Alexander A1 - Scheller, Frieder W. T1 - Electrochemical immunoassays Y1 - 2000 ER - TY - JOUR A1 - Mak, Wing Cheung A1 - Cheung, Kwan Yee A1 - Trau, Dieter A1 - Warsinke, Axel A1 - Scheller, Frieder W. A1 - Renneberg, Reinhard T1 - Electrochemical bioassay utilizing encapsulated electrochemical active microcrystal biolabels N2 - A new approach to perform electrochemical immunoassay based on the utilization of encapsulated microcrystal was developed. The microcrystal labels create a "supernova effect" upon exposure to a desired releasing agent. The microcrystal cores dissolve, and large amounts of signal-generating molecules diffuse across the capsule wall into the outer environment. Layer-by-Layer (LbL) technology was employed for the encapsulation of electrochemical signal- generating microcrystals (ferrocene microcrystals). The encapsulated microcrystals were conjugated with antibody molecules through the adsorption process. The biofunctionalized microcrystals were utilized as a probe for immunoassays. The microcrystal-based label system provided a high-signal molecule to antibody (SIP) ratio of 10(4)-10(5). Microcrystal biolabels with different antibody surface coverage (1.60-5.05 mg m(-2)) were subjected to a solid-phase immunoassay for the detection of mouse immunoglobulin G (M-IgG) molecules. The microcrystal-based immunoassay for the detection of M-IgG performed with microcrystals having antibody surface coverage of 5.05 mg m(-2) showed a sensitivity of 3.93 nA g(- 1) L-1 with a detection limit of 2.82 g L-1 Y1 - 2005 SN - 0003-2700 ER - TY - JOUR A1 - Stöcklein, Walter F. M. A1 - Warsinke, Axel A1 - Micheel, Burkhard A1 - Kempter, Gerhard A1 - Höhne, Wolfgang A1 - Scheller, Frieder W. T1 - Diphenylurea hapten sensing with a monoclonal antibody and its Fab fragment : kinetic and thermodynamic investigations Y1 - 1998 ER - TY - JOUR A1 - Pieper-Fürst, U. A1 - Kleuser, U. A1 - Stöcklein, Walter F. M. A1 - Warsinke, Axel A1 - Scheller, Frieder W. T1 - Detection of subicomolar concentrations of human matrix metalloproteinase-2 by an optical biosensor N2 - We describe in this paper the development of a one-step sandwich assay for the highly sensitive and fast detection of human matrix metalloproteinase (MMP)-2 (EC 3.4.24.24), using surface plasmon resonance (SPR). For the assay, two ligands were selected: monoclonal anti-MMP-2 antibody Ab-2 and the tissue inhibitor of metalloproteinases (TIMP)-2. They were chosen on the basis of (1) their affinities to MMP-2, (2) the efficiency of immobilization to the sensor chip, (3) the efficiency of adsorption to colloidal gold, and (4) the stability of these protein-coated gold particles. The assay included mixing of MMP-2 with antibody Ab-2 adsorbed to colloidal gold with a diameter of about 20 rim and injection into the flowcell of the SPR instrument containing immobilized TIMP-2. By using colloidal gold particles an amplification factor of 114 and a detection limit of 0.5 pM for MMP-2 were obtained. The precision of the assay was high even at low analyte concentrations, the standard deviation being 8.3% for five determinations of 1 pM MMP- 2. No significant binding was observed with the structurally related MMP-9. The assay is far more sensitive and faster than commonly used methods for MMP-2 detection. As TIMP-bound MMP-2 is not detected by this method, the assay can be applied for measuring free MMP-2, reflecting the imbalance of free and inhibitor-bound enzyme in various pathological situations. (C) 2004 Elsevier Inc. All rights reserved Y1 - 2004 ER -