TY  - JOUR
A1  - Schmälzlin, Elmar Gerd
A1  - Moralejo, Benito
A1  - Gersonde, Ingo
A1  - Schleusener, Johannes
A1  - Darvin, Maxim E.
A1  - Thiede, Gisela
A1  - Roth, Martin M.
T1  - Nonscanning large-area Raman imaging for ex vivo/in vivo skin cancer discrimination
JF  - Journal of biomedical optics
N2  - Imaging Raman spectroscopy can be used to identify cancerous tissue. Traditionally, a step-by-step scanning of the sample is applied to generate a Raman image, which, however, is too slow for routine examination of patients. By transferring the technique of integral field spectroscopy (IFS) from astronomy to Raman imaging, it becomes possible to record entire Raman images quickly within a single exposure, without the need for a tedious scanning procedure. An IFS-based Raman imaging setup is presented, which is capable of measuring skin ex vivo or in vivo. It is demonstrated how Raman images of healthy and cancerous skin biopsies were recorded and analyzed. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.
KW  - Raman spectroscopy
KW  - cancer diagnosis
KW  - Raman imaging
KW  - multichannel
KW  - astronomy
KW  - epidermis
KW  - dermis
Y1  - 2018
U6  - https://doi.org/10.1117/1.JBO.23.10.105001
SN  - 1083-3668
SN  - 1560-2281
VL  - 23
IS  - 10
PB  - SPIE
CY  - Bellingham
ER  - 
TY  - JOUR
A1  - Hausmann, Christian
A1  - Zoschke, Christian
A1  - Wolff, Christopher
A1  - Darvin, Maxim E.
A1  - Sochorova, Michaela
A1  - Kovacik, Andrej
A1  - Wanjiku, Barbara
A1  - Schumacher, Fabian
A1  - Tigges, Julia
A1  - Kleuser, Burkhard
A1  - Lademann, Juergen
A1  - Fritsche, Ellen
A1  - Vavrova, Katerina
A1  - Ma, Nan
A1  - Schaefer-Korting, Monika
T1  - Fibroblast origin shapes tissue homeostasis, epidermal differentiation, and drug uptake
JF  - Scientific reports
N2  - Preclinical studies frequently lack predictive value for human conditions. Human cell-based disease models that reflect patient heterogeneity may reduce the high failure rates of preclinical research. Herein, we investigated the impact of primary cell age and body region on skin homeostasis, epidermal differentiation, and drug uptake. Fibroblasts derived from the breast skin of female 20- to 30-yearolds or 60- to 70-year-olds and fibroblasts from juvenile foreskin (<10 years old) were compared in cell monolayers and in reconstructed human skin (RHS). RHS containing aged fibroblasts differed from its juvenile and adult counterparts, especially in terms of the dermal extracellular matrix composition and interleukin-6 levels. The site from which the fibroblasts were derived appeared to alter fibroblast-keratinocyte crosstalk by affecting, among other things, the levels of granulocyte-macrophage colony-stimulating factor. Consequently, the epidermal expression of filaggrin and e-cadherin was increased in RHS containing breast skin fibroblasts, as were lipid levels in the stratum corneum. In conclusion, the region of the body from which fibroblasts are derived appears to affect the epidermal differentiation of RHS, while the age of the fibroblast donors determines the expression of proteins involved in wound healing. Emulating patient heterogeneity in preclinical studies might improve the treatment of age-related skin conditions.
Y1  - 2019
U6  - https://doi.org/10.1038/s41598-019-39770-6
SN  - 2045-2322
VL  - 9
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - JOUR
A1  - Klein, Julia
A1  - Darvin, Maxim E.
A1  - Meinke, Martina C.
A1  - Schweigert, Florian J.
A1  - Müller, Kerstin E.
A1  - Lademann, Jürgen
T1  - Analyses of the correlation between dermal and blood carotenoids in female cattle by optical methods
JF  - Journal of biomedical optics
N2  - Herd health programs for the maintenance of welfare and productivity in cattle need efficient tools for monitoring the health of individual animals. Recent reports demonstrate that the oxidative status is related to various stress conditions in dairy cows. Biomarkers, among other carotenoids, could serve as indicators of stress originating from the environment (e.g., heat stress or sun radiation) or from the animal itself (e.g., disease). To date, only invasive in vitro tests are available to assess the oxidative status in cattle. The present study compares the results of optical noninvasive in vivo measurements of dermal carotenoids in cattle udder skin using an LED-based miniaturized spectroscopic system (MSS) with those obtained by photometric analysis of beta carotene in whole blood samples using a portable device. Correlations between the concentrations of dermal and blood carotenoids were calculated under consideration of the nutritional status of the animals. Significant correlation (R = 0.86) was found for cattle with a moderate to obese body condition. Thus, the blood and skin concentrations of the marker substance beta carotene are comparable under stable stress conditions of the cattle. This demonstrates that the MSS is suitable for noninvasive assessment of dermal carotenoid concentrations in cattle.
KW  - Raman spectroscopy
KW  - reflection spectroscopy
KW  - skin
KW  - antioxidants
KW  - free radicals
Y1  - 2013
U6  - https://doi.org/10.1117/1.JBO.18.6.061219
SN  - 1083-3668
VL  - 18
IS  - 6
PB  - SPIE
CY  - Bellingham
ER  -