TY  - JOUR
A1  - Scott, Shane
A1  - Weiss, Matthias
A1  - Selhuber-Unkel, Christine
A1  - Barooji, Younes F.
A1  - Sabri, Adal
A1  - Erler, Janine T.
A1  - Metzler, Ralf
A1  - Oddershede, Lene B.
T1  - Extracting, quantifying, and comparing dynamical and biomechanical properties of living matter through single particle tracking
JF  - Physical chemistry, chemical physics : a journal of European Chemical Societies
N2  - A panoply of new tools for tracking single particles and molecules has led to an explosion of experimental data, leading to novel insights into physical properties of living matter governing cellular development and function, health and disease. In this Perspective, we present tools to investigate the dynamics and mechanics of living systems from the molecular to cellular scale via single-particle techniques. In particular, we focus on methods to measure, interpret, and analyse complex data sets that are associated with forces, materials properties, transport, and emergent organisation phenomena within biological and soft-matter systems. Current approaches, challenges, and existing solutions in the associated fields are outlined in order to support the growing community of researchers at the interface of physics and the life sciences. Each section focuses not only on the general physical principles and the potential for understanding living matter, but also on details of practical data extraction and analysis, discussing limitations, interpretation, and comparison across different experimental realisations and theoretical frameworks. Particularly relevant results are introduced as examples. While this Perspective describes living matter from a physical perspective, highlighting experimental and theoretical physics techniques relevant for such systems, it is also meant to serve as a solid starting point for researchers in the life sciences interested in the implementation of biophysical methods.
Y1  - 2022
U6  - https://doi.org/10.1039/d2cp01384c
SN  - 1463-9076
SN  - 1463-9084
VL  - 25
IS  - 3
SP  - 1513
EP  - 1537
PB  - RSC Publ.
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Jeon, Jae-Hyung
A1  - Leijnse, Natascha
A1  - Oddershede, Lene B.
A1  - Metzler, Ralf
T1  - Anomalous diffusion and power-law relaxation of the time averaged mean squared displacement in worm-like micellar solutions
JF  - New journal of physics : the open-access journal for physics
N2  - We report the results of single tracer particle tracking by optical tweezers and video microscopy in micellar solutions. From careful analysis in terms of different stochastic models, we show that the polystyrene tracer beads of size 0.52-2.5 mu m after short-time normal diffusion turn over to perform anomalous diffusion of the form < r(2)(t)> similar or equal to t(alpha) with alpha approximate to 0.3. This free anomalous diffusion is ergodic and consistent with a description in terms of the generalized Langevin equation with a power-law memory kernel. With optical tweezers tracking, we unveil a power-law relaxation over several decades in time to the thermal plateau value under the confinement of the harmonic tweezer potential, as predicted previously (Phys. Rev. E 85 021147 (2012)). After the subdiffusive motion in the millisecond range, the motion becomes faster and turns either back to normal Brownian diffusion or to even faster superdiffusion, depending on the size of the tracer beads.
Y1  - 2013
U6  - https://doi.org/10.1088/1367-2630/15/4/045011
SN  - 1367-2630
VL  - 15
IS  - 4
PB  - IOP Publ. Ltd.
CY  - Bristol
ER  -