TY - JOUR A1 - Moradi, Ahmad B. A1 - Carminati, Andrea A1 - Vetterlein, Doris A1 - Vontobel, Peter A1 - Lehmann, Eberhard A1 - Weller, Ulrich A1 - Hopmans, Jan W. A1 - Vogel, Hans-Jörg A1 - Oswald, Sascha T1 - Three-dimensional visualization and quantification of water content in the rhizosphere JF - New phytologist : international journal of plant science N2 - Despite the importance of rhizosphere properties for water flow from soil to roots, there is limited quantitative information on the distribution of water in the rhizosphere of plants. Here, we used neutron tomography to quantify and visualize the water content in the rhizosphere of the plant species chickpea (Cicer arietinum), white lupin (Lupinus albus), and maize (Zea mays) 12 d after planting. We clearly observed increasing soil water contents (h) towards the root surface for all three plant species, as opposed to the usual assumption of decreasing water content. This was true for tap roots and lateral roots of both upper and lower parts of the root system. Furthermore, water gradients around the lower part of the roots were smaller and extended further into bulk soil compared with the upper part, where the gradients in water content were steeper. Incorporating the hydraulic conductivity and water retention parameters of the rhizosphere into our model, we could simulate the gradual changes of h towards the root surface, in agreement with the observations. The modelling result suggests that roots in their rhizosphere may modify the hydraulic properties of soil in a way that improves uptake under dry conditions. KW - extent of rhizosphere KW - modelling KW - neutron tomography KW - rhizosphere hydraulic properties KW - root water uptake KW - soil moisture profile KW - water distribution Y1 - 2011 U6 - https://doi.org/10.1111/j.1469-8137.2011.03826.x SN - 0028-646X VL - 192 IS - 3 SP - 653 EP - 663 PB - Wiley-Blackwell CY - Hoboken ER - TY - JOUR A1 - De Biase, Cecilia A1 - Carminati, Andrea A1 - Oswald, Sascha A1 - Thullner, Martin T1 - Numerical modeling analysis of VOC removal processes in different aerobic vertical flow systems for groundwater remediation JF - Journal of contaminant hydrology N2 - Vertical flow systems filled with porous medium have been shown to efficiently remove volatile organic contaminants (VOCs) from contaminated groundwater. To apply this semi-natural remediation strategy it is however necessary to distinguish between removal due to biodegradation and due to volatile losses to the atmosphere. Especially for (potentially) toxic VOCs, the latter needs to be minimized to limit atmospheric emissions. In this study, numerical simulation was used to investigate quantitatively the removal of volatile organic compounds in two pilot-scale water treatment systems: an unplanted vertical flow filter and a planted one, which could also be called a vertical flow constructed wetland, both used for the treatment of contaminated groundwater. These systems were intermittently loaded with contaminated water containing benzene and MTBE as main VOCs. The highly dynamic but permanently unsaturated conditions in the porous medium facilitated aerobic biodegradation but could lead to volatile emissions of the contaminants. Experimental data from porous material analyses, flow rate measurements, solute tracer and gas tracer test, as well as contaminant concentration measurements at the boundaries of the systems were used to constrain a numerical reactive transport modeling approach. Numerical simulations considered unsaturated water flow, transport of species in the aqueous and the gas phase as well as aerobic degradation processes, which made it possible to quantify the rates of biodegradation and volatile emissions and calculating their contribution to total contaminant removal. A range of degradation rates was determined using experimental results of both systems under two operation modes and validated by field data obtained at different operation modes applied to the filters. For both filters, simulations and experimental data point to high biodegradation rates, if the flow filters have had time to build up their removal capacity. For this case volatile emissions are negligible and total removal can be attributed to biodegradation, only. The simulation study thus supports the use of both of these vertical flow systems for the treatment of groundwater contaminated with VOCs and the use of reactive transport modeling for the assessment of VOCs removal and operation modes in these high performance treatment systems. KW - Benzene KW - Biodegradation KW - MTBE KW - Unsaturated zone KW - Vertical flow filters KW - Volatilization Y1 - 2013 U6 - https://doi.org/10.1016/j.jconhyd.2013.07.007 SN - 0169-7722 SN - 1873-6009 VL - 154 IS - 11 SP - 53 EP - 69 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Moradi, Ahmad B. A1 - Carminati, Andrea A1 - Lamparter, Axel A1 - Woche, Susanne K. A1 - Bachmann, Jörg A1 - Vetterlein, Doris A1 - Vogel, Hans-Jörg A1 - Oswald, Sascha T1 - Is the rhizosphere temporarily water repellent? JF - Vadose zone journal N2 - The rhizosphere has a controlling role in the flow of water and nutrients from soil to plant roots; however, its hydraulic properties are not well understood. As roots grow, they change the pore size distribution of the surrounding soil. Roots release polymeric substances such as mucilage into their rhizosphere. Microorganisms living in the rhizosphere feed on these organic materials and release other polymeric substances into the rhizosphere. The presence of these organic materials might affect the water retention properties and the hydraulic conductivity of the rhizosphere soil during drying and rewetting. We used neutron radiography to monitor the dynamics of water distribution in the rhizosphere of lupin (Lupinus albus L.) plants during a period of drying and rewetting. The rhizosphere was shown to have a higher water content than the bulk soil during the drying period but a lower one during the subsequent rewetting. We evaluated the wettability of the bulk soil and the rhizosphere soil by measuring the contact angle of water in the soil. We found significantly higher contact angles for the rhizosphere soil than the bulk soil after drying, which indicates slight water repellency in the rhizosphere. This explains the lower soil water content in the rhizosphere than the bulk soil after rewetting. Our results suggest that the water holding capacity of the rhizosphere is dynamic and might shift toward higher or lower values than those of the surrounding bulk soil, not affected by roots, depending on the history of drying and rewetting cycles. Y1 - 2012 U6 - https://doi.org/10.2136/vzj2011.0120 SN - 1539-1663 VL - 11 IS - 3 PB - Soil Science Society of America CY - Madison ER - TY - JOUR A1 - Carminati, Andrea A1 - Schneider, Christoph L. A1 - Moradi, Ahmad B. A1 - Zarebanadkouki, Mohsen A1 - Vetterlein, Doris A1 - Vogel, Hans-Jörg A1 - Hildebrandt, Anke A1 - Weller, Ulrich A1 - Schüler, Lennart A1 - Oswald, Sascha T1 - How the rhizosphere may favor water availability to roots JF - Vadose zone journal N2 - Recent studies have shown that rhizosphere hydraulic properties may differ from those of the bulk soil. Specifically, mucilage at the root-soil interface may increase the rhizosphere water holding capacity and hydraulic conductivity during drying. The goal of this study was to point out the implications of such altered rhizosphere hydraulic properties for soil-plant water relations. We addressed this problem through modeling based on a steady-rate approach. We calculated the water flow toward a single root assuming that the rhizosphere and bulk soil were two concentric cylinders having different hydraulic properties. Based on our previous experimental results, we assumed that the rhizosphere had higher water holding capacity and unsaturated conductivity than the bulk soil. The results showed that the water potential gradients in the rhizosphere were much smaller than in the bulk soil. The consequence is that the rhizosphere attenuated and delayed the drop in water potential in the vicinity of the root surface when the soil dried. This led to increased water availability to plants, as well as to higher effective conductivity under unsaturated conditions. The reasons were two: (i) thanks to the high unsaturated conductivity of the rhizosphere, the radius of water uptake was extended from the root to the rhizosphere surface; and (ii) thanks to the high soil water capacity of the rhizosphere, the water depletion in the bulk soil was compensated by water depletion in the rhizosphere. We conclude that under the assumed conditions, the rhizosphere works as an optimal hydraulic conductor and as a reservoir of water that can be taken up when water in the bulk soil becomes limiting. Y1 - 2011 U6 - https://doi.org/10.2136/vzj2010.0113 SN - 1539-1663 VL - 10 IS - 3 SP - 988 EP - 998 PB - Soil Science Society of America CY - Madison ER - TY - JOUR A1 - Rudolph, Nicole A1 - Esser, Hanna G. A1 - Carminati, Andrea A1 - Moradi, Ahmad B. A1 - Hilger, Andre A1 - Kardjilov, Nikolay A1 - Nagl, Stefan A1 - Oswald, Sascha T1 - Dynamic oxygen mapping in the root zone by fluorescence dye imaging combined with neutron radiography JF - Journal of soils and sediments : protection, risk assessment and remediation N2 - The rooted zone of a soil, more precisely the rhizosphere, is a very dynamic system. Some of the key processes are water uptake and root respiration. We have developed a novel method for measuring the real-time distribution of water and oxygen concentration in the rhizosphere as a biogeochemical interface in soil. This enables understanding where and when roots are active in respect to root respiration and water uptake and how the soil responds to it. We used glass containers (15 x 15 x 1 cm), which were filled with a quartz sand mixture. Sensor foils for fluorescence dye imaging of O-2 were installed on the inner side of the containers. A lupine plant was grown in each container for 2 weeks under controlled conditions. Then we took time series of fluorescence images for time-lapsed visualization of oxygen depletion caused by root respiration. Changing water content was mapped in parallel by non-invasive neutron radiography, which yields water content distributions in high spatial resolution. Also it can detect the root system of the lupine plants. By this combined imaging of the samples, a range of water contents and different oxygen concentration levels, both induced by root activities, could be assessed. We monitored the dynamics of these vital parameters induced by roots during a period of several hours. We observed that for high water saturation, the oxygen concentration decreased in parts of the container. The accompanying neutron radiographies gave us the information that these locations are spatially correlated to roots. Therefore, it can be concluded that the observed oxygen deficits close to the roots result from root respiration and show up while re-aeration from atmosphere by gas phase transport is restricted by the high water saturation. Our coupled imaging setup was able to monitor the spatial distribution and temporal dynamics of oxygen and water content in a night and day cycle. This reflects complex plant activities such as photosynthesis, transpiration, and metabolic activities impacting the root-soil interface. Our experimental setup provides the possibility to non-invasively visualize these parameters with high resolution. The particular oxygen imaging method as well as the combination with simultaneously mapping the water content by neutron radiography is a novelty. KW - Fluorescence imaging KW - Neutron radiography KW - Oxygen mapping KW - Rhizosphere KW - Root respiration KW - Water distribution Y1 - 2012 U6 - https://doi.org/10.1007/s11368-011-0407-7 SN - 1439-0108 VL - 12 IS - 1 SP - 63 EP - 74 PB - Springer CY - Heidelberg ER -