TY  - JOUR
A1  - Wojcik, Robin
A1  - Donhauser, Johanna
A1  - Frey, Beat W.
A1  - Holm, Stine
A1  - Holland, Alexandra
A1  - Anesio, Alexandre M.
A1  - Pearce, David A.
A1  - Malard, Lucie
A1  - Wagner, Dirk
A1  - Benning, Liane G.
T1  - Linkages between geochemistry and microbiology in a proglacial terrain in the High Arctic
JF  - Annals of glaciology
N2  - Proglacial environments are ideal for studying the development of soils through the changes of rocks exposed by glacier retreat to weathering and microbial processes. Carbon (C) and nitrogen (N) contents as well as soil pH and soil elemental compositions are thought to be dominant factors structuring the bacterial, archaeal and fungal communities in the early stages of soil ecosystem formation. However, the functional linkages between C and N contents, soil composition and microbial community structures remain poorly understood. Here, we describe a multivariate analysis of geochemical properties and associated microbial community structures between a moraine and a glaciofluvial outwash in the proglacial area of a High Arctic glacier (Longyearbreen, Svalbard). Our results reveal distinct differences in developmental stages and heterogeneity between the moraine and the glaciofluvial outwash. We observed significant relationships between C and N contents, delta C-13(org) and delta N-15 isotopic ratios, weathering and microbial abundance and community structures. We suggest that the observed differences in microbial and geochemical parameters between the moraine and the glaciofluvial outwash are primarily a result of geomorphological variations of the proglacial terrain.
KW  - biogeochemistry
KW  - glacial geomorphology
KW  - glacier chemistry
KW  - microbiology
KW  - processes and landforms of glacial erosion
Y1  - 2018
U6  - https://doi.org/10.1017/aog.2019.1
SN  - 0260-3055
SN  - 1727-5644
VL  - 59
IS  - 77
SP  - 95
EP  - 110
PB  - Cambridge Univ. Press
CY  - Cambridge
ER  - 
TY  - GEN
A1  - Lemaire, Olivier N.
A1  - Infossi, Pascale
A1  - Chaouche, Amine Ali
A1  - Espinosa, Leon
A1  - Leimkühler, Silke
A1  - Giudici-Orticoni, Marie-Thérèse
A1  - Méjean, Vincent
A1  - Iobbi-Nivol, Chantal
T1  - Small membranous proteins of the TorE/NapE family, crutches for cognate respiratory systems in Proteobacteria
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - In this report, we investigate small proteins involved in bacterial alternative respiratory systems that improve the enzymatic efficiency through better anchorage and multimerization of membrane components. Using the small protein TorE of the respiratory TMAO reductase system as a model, we discovered that TorE is part of a subfamily of small proteins that are present in proteobacteria in which they play a similar role for bacterial respiratory systems. We reveal by microscopy that, in Shewanella oneidensis MR1, alternative respiratory systems are evenly distributed in the membrane contrary to what has been described for Escherichia coli. Thus, the better efficiency of the respiratory systems observed in the presence of the small proteins is not due to a specific localization in the membrane, but rather to the formation of membranous complexes formed by TorE homologs with their c-type cytochrome partner protein. By an in vivo approach combining Clear Native electrophoresis and fluorescent translational fusions, we determined the 4: 4 stoichiometry of the complexes. In addition, mild solubilization of the cytochrome indicates that the presence of the small protein reinforces its anchoring to the membrane. Therefore, assembly of the complex induced by this small protein improves the efficiency of the respiratory system.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 933 
KW  - trimethylamine n-oxide
KW  - molybdenum cofactor biosynthesis
KW  - cytochrome bd oxidase
KW  - c-type cytochromes
KW  - escherichia-coli
KW  - swiss-model
KW  - native electrophoresis
KW  - mutational analysis
KW  - reductase
KW  - nitrate
KW  - microbiology
KW  - microbiology techniques
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-459208
SN  - 1866-8372
IS  - 933
ER  -