TY  - GEN
A1  - Fernando, Raquel
A1  - Drescher, Cathleen
A1  - Deubel, Stefanie
A1  - Grune, Tilman
A1  - Castro, Jose Pedro
T1  - Distinct proteasomal activity for fast and slow twitch skeletal muscle during aging
T2  - Free radical biology and medicine : the official journal of the Oxygen Society, a constituent member of the International Society for Free Radical Research
N2  - Skeletal muscle alterations during aging lead to dysfunctional metabolism, correlating with frailty and early mortality. The loss of proteostasis is a hallmark of aging. Whether proteostasis loss plays a role in muscle aging remains elusive. To address this question we collected muscles, Soleus (SOL, type I) and Extensor digitorum longus (EDL, type II), from young (4 months) and old (25 months) C57BL/6 mice and evaluated the proteasomal system. Initial work showed decreased 26 S activity in old SOL. EDL displayed lower proteasomal activity in both ages compared to any of the SOL ages. Moreover, in order to understand if during aging there is the so-called “fiber switch from fast-to-slow”, we performed western blots against sMHC and fMHC (slow and fast myosin heavy chain, respectively). Preliminary results suggest that young SOL is composed by slow twitch fibers but also contains fast twitch fibers, while young EDL seems to be mostly composed by fast twitch fibers that level down during aging, suggesting the switch. As a conclusion, EDL seems to have less proteasomal activity, however, if this is a contributor or a consequence to the muscle fiber switch during aging still needs further investigation.
Y1  - 2018
U6  - https://doi.org/10.1016/j.freeradbiomed.2018.04.393
SN  - 0891-5849
SN  - 1873-4596
VL  - 120
SP  - S119
EP  - S119
PB  - Elsevier
CY  - New York
ER  -