TY  - JOUR
A1  - Sadowska, Aleksandra
A1  - Touli, Ermioni
A1  - Hitzl, Wolfgang
A1  - Greutert, Helen
A1  - Ferguson, Stephen J.
A1  - Würtz-Kozak, Karin
A1  - Hausmann, Oliver N.
T1  - Inflammaging in cervical and lumbar degenerated intervertebral discs
BT  - analysis of proinflammatory cytokine and TRP channel expression
JF  - European Spine Journal
N2  - To investigate and compare the occurrence of inflammatory processes in the sites of disc degeneration in the lumbar and cervical spine by a gene array and subsequent qPCR and to investigate the mechanistic involvement of transient receptor potential channels TRPC6 and TRPV4. The gene expression of inflammatory cytokines and TRP channels was measured in human disc samples obtained from patients undergoing discectomy at the cervical (n = 24) or lumbar (n = 27) spine for degenerative disc disease (DDD) and disc herniation (DH) and analyzed for differences with regard to spinal level, IVD degeneration grade, Modic grade, age, sex, disc region and surgical extent. Aside from genes with known implication in DDD and DH, four previously unreported genes from the interferon and TRP families (IFNA1, IFNA8, IFNB1, TRPC6) could be detected. A correlation between gene expression and age (IL-15) and IVD degeneration grade (IFNA1, IL-6, IL-15, TRPC6), but not Modic grade, was identified. Significant differences were detected between cervical and lumbar discs (IL-15), nucleus and annulus (IL-6, TNF-alpha, TRPC6), single-level and multi-level surgery (IL-6, IL-8) as well as DDD and DH (IL-8), while sex had no effect. Multiple gene-gene pair correlations, either between different cytokines or between cytokines and TRP channels, exist in the disc. This study supports the relevance of IL-6 and IL-8 in disc diseases, but furthermore points toward a possible pathological role of IL-15 and type I interferons, as well as a mechanistic role of TRPC6. With limited differences in the inflammatory profile of cervical and lumbar discs, novel anti-inflammatory or TRP-modulatory strategies for the treatment of disc pathologies may be applicable independent of the spinal region.
KW  - Cervical and lumbar discs
KW  - Degenerative disc disease (DDD) and disc herniation (DH)
KW  - Inflammaging
KW  - Inflammation
KW  - Intervertebral disc
KW  - Transient receptor potential (TRP) channel
Y1  - 2017
U6  - https://doi.org/10.1007/s00586-017-5360-8
SN  - 0940-6719
SN  - 1432-0932
VL  - 27
IS  - 3
SP  - 564
EP  - 577
PB  - Springer
CY  - New York
ER  - 
TY  - JOUR
A1  - Koelman, Liselot A.
A1  - Pivovarova-Ramich, Olga
A1  - Pfeiffer, Andreas F. H.
A1  - Grune, Tilman
A1  - Aleksandrova, Krasimira
T1  - Cytokines for evaluation of chronic inflammatory status in ageing research
BT  - reliability and phenotypic characterisation
JF  - Immunity & Ageing
N2  - Background: There is a growing interest in the role of inflammageing for chronic disease development. Cytokines are potent soluble immune mediators that can be used as target biomarkers of inflammageing; however, their measurement in human samples has been challenging. This study aimed to assess the reliability of a pro- and anti-inflammatory cytokine panel in a sample of healthy people measured with a novel electrochemiluminescent multiplex immunoassay platform (Meso Scale Discovery, MSD), and to characterize their associations with metabolic and inflammatory phenotypes.
KW  - Reliability
KW  - Cytokines
KW  - Multiplex platforms
KW  - Inflammaging
KW  - Biomarkers
KW  - Ageing
KW  - BMI
Y1  - 2019
U6  - https://doi.org/10.1186/s12979-019-0151-1
SN  - 1742-4933
VL  - 16
PB  - BMC
CY  - London
ER  -