TY  - GEN
A1  - Randall, Matthew J.
A1  - Jüngel, Astrid
A1  - Rimann, Markus
A1  - Wuertz-Kozak, Karin
T1  - Advances in the biofabrication of 3D skin in vitro
BT  - healthy and pathological models
T2  - Postprints der Universität Potsdam : Humanwissenschaftliche Reihe
N2  - The relevance for in vitro three-dimensional (3D) tissue culture of skin has been present for almost a century. From using skin biopsies in organ culture, to vascularized organotypic full-thickness reconstructed human skin equivalents, in vitro tissue regeneration of 3D skin has reached a golden era. However, the reconstruction of 3D skin still has room to grow and develop. The need for reproducible methodology, physiological structures and tissue architecture, and perfusable vasculature are only recently becoming a reality, though the addition of more complex structures such as glands and tactile corpuscles require advanced technologies. In this review, we will discuss the current methodology for biofabrication of 3D skin models and highlight the advantages and disadvantages of the existing systems as well as emphasize how new techniques can aid in the production of a truly physiologically relevant skin construct for preclinical innovation.
T3  - Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe - 680 
KW  - 3D tissue model
KW  - skin
KW  - in vitro
KW  - bioprinting
KW  - electrospinning
KW  - skin disease
KW  - biofabrication
KW  - preclinical testing
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-468844
SN  - 1866-8364
IS  - 680
ER  - 
TY  - GEN
A1  - Olmer, Ruth
A1  - Engels, Lena
A1  - Usman, Abdulai
A1  - Menke, Sandra
A1  - Malik, Muhammad Nasir Hayat
A1  - Pessler, Frank
A1  - Göhring, Gudrun
A1  - Bornhorst, Dorothee
A1  - Bolten, Svenja
A1  - Abdelilah-Seyfried, Salim
A1  - Scheper, Thomas
A1  - Kempf, Henning
A1  - Zweigerdt, Robert
A1  - Martin, Ulrich
T1  - Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Endothelial cells (ECs) are involved in a variety of cellular responses. As multifunctional components of vascular structures, endothelial (progenitor) cells have been utilized in cellular therapies and are required as an important cellular component of engineered tissue constructs and in vitro disease models. Although primary ECs from different sources are readily isolated and expanded, cell quantity and quality in terms of functionality and karyotype stability is limited. ECs derived from human induced pluripotent stem cells (hiPSCs) represent an alternative and potentially superior cell source, but traditional culture approaches and 2D differentiation protocols hardly allow for production of large cell numbers. Aiming at the production of ECs, we have developed a robust approach for efficient endothelial differentiation of hiPSCs in scalable suspension culture. The established protocol results in relevant numbers of ECs for regenerative approaches and industrial applications that show in vitro proliferation capacity and a high degree of chromosomal stability.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1182 
KW  - virus infection
KW  - progenitor cells
KW  - in vitro
KW  - telomere dysfunction
KW  - cord blood
KW  - cardiomyogenic differentiation
KW  - angiogenesis
KW  - efficient
KW  - aberrations
KW  - expression
Y1  - 2017
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-427095
SN  - 1866-8372
IS  - 5
ER  -