TY  - JOUR
A1  - Vijayakrishnan, Balakumar
A1  - Issaree, Arisara
A1  - Corilo, Yuri E.
A1  - Ferreira, Christina Ramires
A1  - Eberlin, Marcos N.
A1  - Peter, Martin G.
T1  - MSn of the six isomers of (GlcN)(2)(GlcNAc)(2) aminoglucan tetrasaccharides (diacetylchitotetraoses) rules of fragmentation for the sodiated molecules and application to sequence analysis of hetero-chitooligosaccharides
JF  - Carbohydrate polymers : an international journal devoted to scientific and technological aspects of industrially important polysaccharides
N2  - The six possible isomers of di-N-acetylchitotetraoses [AADD, ADDA, ADAD, DADA, DAAD, and DDAA, where D stands for 2-amino-2-deoxy-3-D-glucose (GlcN) and A for 2-acetamido-2-deoxy-beta-D-glucose (GlcNAc)] were analyzed by ESI(+)-MSn. Collision induced dissociation via MSn experiments were performed for the sodiated molecules of m/z 769 [M+Na](+) for each isomer, and fragments were generated mainly by glycosidic bond and cross-ring cleavages. Rules of fragmentation were then established. A reducing end D residue yields the (O.2)A(4) cross-ring [M-59+Na](+) fragment of m/z 710 as the most abundant, whereas isomers containing a reducing end A prefer to lose water to form the [M-18+Na](+) ion of m/z 751, as well as abundant (O.2)A(4) cross-ring [M-101+Na](+) fragments of m/z 668 and B-3 [M-221+Na](+) ions of m/z 548. MS3 of C- and Y-type ions shows analogous fragmentation behaviour that allows identification of the reducing end next-neighbour residue. Due to gas-phase anchimeric assistance, B-type cleavage between the glycosidic oxygen and the anomeric carbon atom is favoured when the glycon is an A residue. Relative ion abundances are generally in the order B >> C > Y, but may vary depending on the next neighbour towards the non-reducing end. These fragmentation rules were used for partial sequence analysis of hetero-chitooligosaccharides of the composition D(2)A(3), D(3)A(3), D(2)A(4), D(4)A(3), and D(3)A(4).
KW  - Chitosan
KW  - Fragmentation
KW  - Oligosaccharides
KW  - Sequence analysis
KW  - Tandem mass spectrometry
Y1  - 2011
U6  - https://doi.org/10.1016/j.carbpol.2010.04.041
SN  - 0144-8617
VL  - 84
IS  - 2
SP  - 713
EP  - 726
PB  - Elsevier
CY  - Oxford
ER  - 
TY  - GEN
A1  - Oliveira Jr, E. N.
A1  - Gueddari, Nour E. El
A1  - Moerschbacher, Bruno M.
A1  - Peter, Martin
A1  - Franco, Telma
T1  - Growth of phytopathogenic fungi in the presence of partially acetylated chitooligosaccharides
N2  - Four phytopathogenic fungi were cultivated up to six days in media containing chitooligosaccharide mixtures differing in average DP and FA. The three different mixtures were named Q3 (which contained oligosaccharides ofDP2–DP10, withDP2–DP7 asmain components), Q2 (which contained oligosaccharides of DP2–DP12, with DP2–DP10 as main components) and Q1 (which derived from Q2 and contained oligomers of DP5–DP8 with hexamer and a heptamer as the main components). The novel aspect of this work is the description of the effect of mixtures of oligosaccharides with different and known composition on fungal growth rates. The growth rate of Alternaria alternata and Rhizopus stolonifer was initially inhibited by Q3 and Q2 at higher concentrations. Q1 had a growth stimulating effect on these two fungi. Growth of Botrytis cinerea was inhibited by Q3 and Q2, while Q1 had no effect on the growth of this fungus. Growth of Penicillium expansum was only slightly inhibited by higher concentrations of sample Q3, while Q2 and Q1 had no effect. The inhibition of growth rates or their resistance toward chitooligosaccharides correlated with the absence or presence of chitinolytic enzymes in the culture media, respectively.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 105 
KW  - Chitosan
KW  - Chitinase
KW  - Fungi
KW  - Oligosaccharides
KW  - Phytopathogens
Y1  - 2008
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-42646
ER  -