TY  - GEN
A1  - Westendorf, Christian
A1  - Bae, Albert J.
A1  - Erlenkamper, Christoph
A1  - Galland, Edouard
A1  - Franck, Carl
A1  - Bodenschatz, Eberhard
A1  - Beta, Carsten
T1  - Live cell flattening
BT  - traditional and novel approaches
T2  - Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe
N2  - Eukaryotic cell flattening is valuable for improving microscopic observations, ranging from bright field (BF) to total internal reflection fluorescence (TIRF) microscopy. Fundamental processes, such as mitosis and in vivo actin polymerization, have been investigated using these techniques. Here, we review the well known agar overlayer protocol and the oil overlay method. In addition, we present more elaborate microfluidics-based techniques that provide us with a greater level of control. We demonstrate these techniques on the social amoebae Dictyostelium discoideum, comparing the advantages and disadvantages of each method.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 835 
KW  - PDMS
KW  - microfluidic device
KW  - lower channel
KW  - total internal reflection fluorescence
KW  - total internal reflection fluorescence microscopy
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-428311
SN  - 1866-8372
IS  - 835
ER  -