TY  - GEN
A1  - Radbruch, Moritz
A1  - Pischon, Hannah
A1  - Ostrowski, Anja
A1  - Volz, Pierre
A1  - Brodwolf, Robert
A1  - Neumann, Falko
A1  - Unbehauen, Michael
A1  - Kleuser, Burkhard
A1  - Haag, Rainer
A1  - Ma, Nan
A1  - Alexiev, Ulrike
A1  - Mundhenk, Lars
A1  - Gruber, Achim D.
T1  - Dendritic core-multishell nanocarriers in murine models of healthy and atopic skin
T2  - Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe
N2  - Dendritic hPG-amid-C18-mPEG core-multishell nanocarriers (CMS) represent a novel class of unimolecular micelles that hold great potential as drug transporters, e. g., to facilitate topical therapy in skin diseases. Atopic dermatitis is among the most common inflammatory skin disorders with complex barrier alterations which may affect the efficacy of topical treatment.

Here, we tested the penetration behavior and identified target structures of unloaded CMS after topical administration in healthy mice and in mice with oxazolone-induced atopic dermatitis. We further examined whole body distribution and possible systemic side effects after simulating high dosage dermal penetration by subcutaneous injection.

Following topical administration, CMS accumulated in the stratum corneum without penetration into deeper viable epidermal layers. The same was observed in atopic dermatitis mice, indicating that barrier alterations in atopic dermatitis had no influence on the penetration of CMS. Following subcutaneous injection, CMS were deposited in the regional lymph nodes as well as in liver, spleen, lung, and kidney. However, in vitro toxicity tests, clinical data, and morphometry-assisted histopathological analyses yielded no evidence of any toxic or otherwise adverse local or systemic effects of CMS, nor did they affect the severity or course of atopic dermatitis.

Taken together, CMS accumulate in the stratum corneum in both healthy and inflammatory skin and appear to be highly biocompatible in the mouse even under conditions of atopic dermatitis and thus could potentially serve to create a depot for anti-inflammatory drugs in the skin.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 724 
KW  - CMS
KW  - skin
KW  - topical treatment
KW  - dermal delivery
KW  - atopic dermatitis
KW  - oxazolone
KW  - fluorescence lifetime imaging microscopy
KW  - nanomaterials
KW  - multi-domain nanoparticles
KW  - penetration enhancement
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-430136
SN  - 1866-8372
IS  - 724
ER  - 
TY  - GEN
A1  - Neffe, Axel T.
A1  - von Rüsten-Lange, Maik
A1  - Braune, Steffen
A1  - Lützow, Karola
A1  - Roch, Toralf
A1  - Richau, Klaus
A1  - Krüger, Anne
A1  - Becherer, Tobias
A1  - Thünemann, Andreas F.
A1  - Jung, Friedrich
A1  - Haag, Rainer
A1  - Lendlein, Andreas
T1  - Multivalent grafting of hyperbranched oligo- and polyglycerols shielding rough membranes to mediate hemocompatibility
N2  - Hemocompatible materials are needed for internal and extracorporeal biomedical applications, which should be realizable by reducing protein and thrombocyte adhesion to such materials. Polyethers have been demonstrated to be highly efficient in this respect on smooth surfaces. Here, we investigate the grafting of oligo- and polyglycerols to rough poly(ether imide) membranes as a polymer relevant to biomedical applications and show the reduction of protein and thrombocyte adhesion as well as thrombocyte activation. It could be demonstrated that, by performing surface grafting with oligo- and polyglycerols of relatively high polydispersity (>1.5) and several reactive groups for surface anchoring, full surface shielding can be reached, which leads to reduced protein adsorption of albumin and fibrinogen. In addition, adherent thrombocytes were not activated. This could be clearly shown by immunostaining adherent proteins and analyzing the thrombocyte covered area. The presented work provides an important strategy for the development of application relevant hemocompatible 3D structured materials.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 285 
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-99444
ER  -