TY  - JOUR
A1  - Zeng, Ting
A1  - Pankratov, Dmitry
A1  - Falk, Magnus
A1  - Leimkühler, Silke
A1  - Shleev, Sergey
A1  - Wollenberger, Ursula
T1  - Miniature direct electron transfer based sulphite/oxygen enzymatic fuel cells
JF  - Biosensors and bioelectronics : the principal international journal devoted to research, design development and application of biosensors and bioelectronics
N2  - A direct electron transfer (DET) based sulphite/oxygen biofuel cell is reported that utilises human sulphite oxidase (hSOx) and Myrothecium verrucaria bilirubin oxidase (MvBOx) and nanostructured gold electrodes. For bioanode construction, the nanostructured gold microelectrodes were further modified with 3,3'-dithiodipropionic acid di(N-hydroxysuccinimide ester) to which polyethylene imine was covalently attached. hSOx was adsorbed onto this chemically modified nanostructured electrode with high surface loading of electroactive enzyme and in presence of sulphite high anodic bioelectrocatalytic currents were generated with an onset potential of 0.05 V vs. NHE. The biocathode contained MyBOx directly adsorbed to the deposited gold nanoparticles for cathodic oxygen reduction starting at 0.71 V vs. NHE. Both enzyme electrodes were integrated to a DET-type biofuel cell. Power densities of 8 and 1 mu W cm(-2) were achieved at 0.15 V and 0.45 V of cell voltages, respectively, with the membrane based biodevices under aerobic conditions. (C) 2014 Elsevier B.V. All rights reserved.
KW  - Enzymatic fuel cell
KW  - Microscale electrode
KW  - Direct electron transfer
KW  - Sulphite oxidase
KW  - Bilirubin oxidase
Y1  - 2015
U6  - https://doi.org/10.1016/j.bios.2014.10.080
SN  - 0956-5663
SN  - 1873-4235
VL  - 66
SP  - 39
EP  - 42
PB  - Elsevier
CY  - Oxford
ER  -