TY - JOUR A1 - Annunziata, Maria Grazia A1 - Apelt, Federico A1 - Carillo, Petronia A1 - Krause, Ursula A1 - Feil, Regina A1 - Mengin, Virginie A1 - Lauxmann, Martin A. A1 - Koehl, Karin A1 - Nikoloski, Zoran A1 - Stitt, Mark A1 - Lunn, John Edward T1 - Getting back to nature: a reality check for experiments in controlled environments JF - Journal of experimental botany N2 - Irradiance from sunlight changes in a sinusoidal manner during the day, with irregular fluctuations due to clouds, and light-dark shifts at dawn and dusk are gradual. Experiments in controlled environments typically expose plants to constant irradiance during the day and abrupt light-dark transitions. To compare the effects on metabolism of sunlight versus artificial light regimes, Arabidopsis thaliana plants were grown in a naturally illuminated greenhouse around the vernal equinox, and in controlled environment chambers with a 12-h photoperiod and either constant or sinusoidal light profiles, using either white fluorescent tubes or light-emitting diodes (LEDs) tuned to a sunlight-like spectrum as the light source. Rosettes were sampled throughout a 24-h diurnal cycle for metabolite analysis. The diurnal metabolite profiles revealed that carbon and nitrogen metabolism differed significantly between sunlight and artificial light conditions. The variability of sunlight within and between days could be a factor underlying these differences. Pairwise comparisons of the artificial light sources (fluorescent versus LED) or the light profiles (constant versus sinusoidal) showed much smaller differences. The data indicate that energy-efficient LED lighting is an acceptable alternative to fluorescent lights, but results obtained from plants grown with either type of artificial lighting might not be representative of natural conditions. KW - Amino acid KW - Arabidopsis thaliana KW - controlled environment KW - LED lighting KW - visible light spectrum KW - organic acid KW - starch KW - sucrose KW - trehalose 6-phosphate Y1 - 2017 U6 - https://doi.org/10.1093/jxb/erx220 SN - 0022-0957 SN - 1460-2431 VL - 68 SP - 4463 EP - 4477 PB - Oxford Univ. Press CY - Oxford ER - TY - JOUR A1 - Menzel, Philip A1 - Gaye, Birgit A1 - Mishra, Praveen Kumar A1 - Anoop, Ambili A1 - Basavaiah, Nathani A1 - Marwan, Norbert A1 - Plessen, Birgit A1 - Prasad, Sushma A1 - Riedel, Nils A1 - Stebich, Martina A1 - Wiesner, Martin G. T1 - Linking Holocene drying trends from Lonar Lake in monsoonal central India to North Atlantic cooling events JF - Palaeogeography, palaeoclimatology, palaeoecology : an international journal for the geo-sciences N2 - We present the results of biogeochemical and mineralogical analyses on a sediment core that covers the Holocene sedimentation history of the climatically sensitive, closed, saline, and alkaline Lonar Lake in the core monsoon zone in central India. We compare our results of C/N ratios, stable carbon and nitrogen isotopes, grain-size, as well as amino acid derived degradation proxies with climatically sensitive proxies of other records from South Asia and the North Atlantic region. The comparison reveals some more or less contemporaneous climate shifts. At Lonar Lake, a general long term climate transition from wet conditions during the early Holocene to drier conditions during the late Holocene, delineating the insolation curve, can be reconstructed. In addition to the previously identified periods of prolonged drought during 4.6-3.9 and 2.0-0.6 cal ka that have been attributed to temperature changes in the Indo Pacific Warm Pool, several additional phases of shorter term climate alteration superimposed upon the general climate trend can be identified. These correlate with cold phases in the North Atlantic region. The most pronounced climate deteriorations indicated by our data occurred during 62-5.2,4.6-3.9, and 2.0-0.6 cal ka BP. The strong dry phase between 4.6 and 3.9 cal ka BP at Lonar Lake corroborates the hypothesis that severe climate deterioration contributed to the decline of the Indus Civilisation about 3.9 ka BP. (C) 2014 Elsevier B.V. All rights reserved. KW - Lake sediment KW - Indian monsoon KW - Holocene KW - Climate reconstruction KW - Stable carbon isotope KW - Amino acid Y1 - 2014 U6 - https://doi.org/10.1016/j.palaeo.2014.05.044 SN - 0031-0182 SN - 1872-616X VL - 410 SP - 164 EP - 178 PB - Elsevier CY - Amsterdam ER -