TY  - JOUR
A1  - Lisso, Janina
A1  - Altmann, Thomas
A1  - Müssig, Carsten
T1  - Metabolic changes in fruits of the tomato d(x) mutant
JF  - Phytochemistry : an international journal of plant biochemistry
KW  - Solanum lycopersicum
KW  - Solanaceae
KW  - tomato
KW  - brassinosteroid
KW  - primary metabolism
KW  - fruit
Y1  - 2006
U6  - https://doi.org/10.1016/j.phytochem.2006.07.008
SN  - 0031-9422
VL  - 67
IS  - 20
SP  - 2232
EP  - 2238
PB  - Elsevier
CY  - Oxford
ER  - 
TY  - GEN
A1  - Huu, Cuong Nguyen
A1  - Plaschil, Sylvia
A1  - Himmelbach, Axel
A1  - Kappel, Christian
A1  - Lenhard, Michael
T1  - Female self-incompatibility type in heterostylous Primula is determined by the brassinosteroid-inactivating cytochrome P450 CYP734A50
T2  - Current biology
N2  - Most flowering plants are hermaphrodites, with flowers having both male and female reproductive organs. One widespread adaptation to limit self-fertilization is self-incompatibility (SI), where self-pollen fails to fertilize ovules.(1,2) In homomorphic SI, many morphologically indistinguishable mating types are found, although in heteromorphic SI, the two or three mating types are associated with different floral morphologies.(3-6) In heterostylous Primula, a hemizygous supergene determines a short-styled S-morph and a long-styled L-morph, corresponding to two different mating types, and full seed set only results from inter morph crosses.(7-9) Style length is controlled by the brassinosteroid (BR)-inactivating cytochrome P450 CYP734A50,(10) yet it remains unclear what defines the male and female incompatibility types. Here, we show that CYP734A50 also determines the female incompatibility type. Inactivating CYP734A50 converts short S-morph styles into long styles with the same incompatibility behavior as L-morph styles, and this effect can be mimicked by exogenous BR treatment. In vitro responses of S-and L-morph pollen grains and pollen tubes to increasing BR levels could only partly explain their different in vivo behavior, suggesting both direct and indirect effects of the different BR levels in S-versus L-morph stigmas and styles in controlling pollen performance. This BR-mediated SI provides a novel mechanism for preventing self-fertilization. The joint control of morphology and SI by CYP734A50 has important implications for the evolutionary buildup of the heterostylous syndrome and provides a straightforward explanation for why essentially all of the derived self-compatible homostylous Primula species are long homostyles.(11)
KW  - heteromorphic self-incompatibility
KW  - heterostyly
KW  - Primula forbesii
KW  - brassinosteroid
KW  - CYP734A50
KW  - supergene
KW  - pleiotropy
Y1  - 2022
U6  - https://doi.org/10.1016/j.cub.2021.11.046
SN  - 0960-9822
SN  - 1879-0445
VL  - 32
IS  - 3
SP  - 671
EP  - 676, E1-E5
PB  - Cell Press
CY  - Cambridge, Mass.
ER  - 
TY  - JOUR
A1  - Shahnejat-Bushehri, Sara
A1  - Allu, Annapurna Devi
A1  - Mehterov, Nikolay
A1  - Thirumalaikumar, Venkatesh P.
A1  - Alseekh, Saleh
A1  - Fernie, Alisdair R.
A1  - Mueller-Roeber, Bernd
A1  - Balazadeh, Salma
T1  - Arabidopsis NAC Transcription Factor JUNGBRUNNEN1 Exerts Conserved Control Over Gibberellin and Brassinosteroid Metabolism and Signaling Genes in Tomato
JF  - Frontiers in plant science
N2  - The Arabidopsis thaliana NAC transcription factor JUNGBRUNNEN1 (AtJUB1) regulates growth by directly repressing GA3ox1 and DWF4, two key genes involved in gibberellin (GA) and brassinosteroid (BR) biosynthesis, respectively, leading to GA and BR deficiency phenotypes. AtJUB1 also reduces the expression of PIF4, a bHLH transcription factor that positively controls cell elongation, while it stimulates the expression of DELLA genes, which are important repressors of growth. Here, we extend our previous findings by demonstrating that AtJUB1 induces similar GA and BR deficiency phenotypes and changes in gene expression when overexpressed in tomato (Solanum lycopersicum). Importantly, and in accordance with the growth phenotypes observed, AtJUB1 inhibits the expression of growth-supporting genes, namely the tomato orthologs of GA3ox1, DWF4 and PIF4, but activates the expression of DELLA orthologs, by directly binding to their promoters. Overexpression of AtJUB1 in tomato delays fruit ripening, which is accompanied by reduced expression of several ripeningrelated genes, and leads to an increase in the levels of various amino acids (mostly proline, beta-alanine, and phenylalanine), gamma-aminobutyric acid (GABA), and major organic acids including glutamic acid and aspartic acid. The fact that AtJUB1 exerts an inhibitory effect on the GA/BR biosynthesis and PIF4 genes but acts as a direct activator of DELLA genes in both, Arabidopsis and tomato, strongly supports the model that the molecular constituents of the JUNGBRUNNEN1 growth control module are considerably conserved across species.
KW  - Arabidopsis
KW  - tomato
KW  - fruit
KW  - growth
KW  - transcription factor
KW  - gibberellic acid
KW  - brassinosteroid
KW  - DELLA proteins
Y1  - 2017
U6  - https://doi.org/10.3389/fpls.2017.00214
SN  - 1664-462X
VL  - 8
PB  - Frontiers Research Foundation
CY  - Lausanne
ER  -