TY  - JOUR
A1  - Wischke, Christian
A1  - Baehr, Elen
A1  - Racheva, Miroslava
A1  - Heuchel, Matthias
A1  - Weigel, Thomas
A1  - Lendlein, Andreas
T1  - Surface immobilization strategies for tyrosinase as biocatalyst applicable to polymer network synthesis
JF  - MRS Advances
N2  - Enzymes have recently attracted increasing attention in material research based on their capacity to catalyze the conversion of polymer-bound moieties for synthesizing polymer networks, particularly bulk hydrogels. hi this study. the surface immobilization of a relevant enzyme. mushroom tyrosinase, should be explored using glass as model surface. In a first step. the glass support was functionalized with silanes to introduce either amine or carboxyl groups, as confirmed e.g. by X-ray photoelectron spectroscopy. By applying glutaraldehyde and EDC/NHS chemistry, respectively, surfaces have been activated for subsequent successful coupling of tyrosinase. Via protein hydrolysis and amino acid characterization by HPLC, the quantity of bound tyrosinase was shown to correspond to a full surface coverage. Based on the visualized enzymatic conversion of a test substrate at the glass support. the functionalized surfaces may be explored for surface-associated material synthesis in the future.
Y1  - 2018
U6  - https://doi.org/10.1557/adv.2018.630
SN  - 2059-8521
VL  - 3
IS  - 63
SP  - 3875
EP  - 3881
PB  - Cambridge Univ. Press
CY  - New York
ER  - 
TY  - CHAP
A1  - Friess, Fabian
A1  - Lendlein, Andreas
A1  - Wischke, Christian
T1  - Investigating side-reactions during UV-induced preparation of oligo(epsilon-caprolactone) based shape-memory polymer networks
T2  - Abstracts of papers : joint conference / The Chemical Institute of Cananda, CIC, American Chemical Society, ACS
Y1  - 2013
SN  - 0065-7727
VL  - 245
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - JOUR
A1  - Dal Bianco, Andrea
A1  - Wischke, Christian
A1  - Zhou, Shuo
A1  - Lendlein, Andreas
T1  - Controlling surface properties and permeability of polyglycerol network films
JF  - Polymers for advanced technologies
N2  - While branched polyglycerol (PG)-based molecules are well established as hydrophilic particles, the capacity of utilizing PG in bulk materials and opportunities arising by their further surface functionalization have only recently been considered. Here we investigated how the mold used in PG network synthesis may affect surface composition and how the permeability of substances through PG can be controlled by altering network structure, i.e. introducing 20mol% oligoethylene glycol (OEG) bifunctional spacer molecules. Overall, PG-based bulk network materials were shown to be tailorable, hydrophilic, low swelling and relatively stiff polyether-based materials, with low impact of salt onto material properties. Based on these features, but also on the principal capacity of free hydroxyl groups to be used for functionalization reactions, these materials may be an interesting platform for medical and technical applications, e.g. as diffusion-rate controlling membrane in aqueous environment. Copyright (c) 2016 John Wiley & Sons, Ltd.
KW  - polyglycerol
KW  - surface properties
KW  - diffusion
KW  - network structure
Y1  - 2017
U6  - https://doi.org/10.1002/pat.3917
SN  - 1042-7147
SN  - 1099-1581
VL  - 28
SP  - 1263
EP  - 1268
PB  - Wiley
CY  - Hoboken
ER  -