TY  - JOUR
A1  - Sulpice, Ronan
A1  - Pyl, Eva-Theresa
A1  - Ishihara, Hirofumi
A1  - Trenkamp, Sandra
A1  - Steinfath, Matthias
A1  - Witucka-Wall, Hanna
A1  - Gibon, Yves
A1  - Usadel, Björn
A1  - Poree, Fabien
A1  - Piques, Maria Conceicao
A1  - von Korff, Maria
A1  - Steinhauser, Marie Caroline
A1  - Keurentjes, Joost J. B.
A1  - Guenther, Manuela
A1  - Hoehne, Melanie
A1  - Selbig, Joachim
A1  - Fernie, Alisdair R.
A1  - Altmann, Thomas
A1  - Stitt, Mark
T1  - Starch as a major integrator in the regulation of plant growth
N2  - Rising demand for food and bioenergy makes it imperative to breed for increased crop yield. Vegetative plant growth could be driven by resource acquisition or developmental programs. Metabolite profiling in 94 Arabidopsis accessions revealed that biomass correlates negatively with many metabolites, especially starch. Starch accumulates in the light and is degraded at night to provide a sustained supply of carbon for growth. Multivariate analysis revealed that starch is an integrator of the overall metabolic response. We hypothesized that this reflects variation in a regulatory network that balances growth with the carbon supply. Transcript profiling in 21 accessions revealed coordinated changes of transcripts of more than 70 carbon-regulated genes and identified 2 genes (myo-inositol-1- phosphate synthase, a Kelch-domain protein) whose transcripts correlate with biomass. The impact of allelic variation at these 2 loci was shown by association mapping, identifying them as candidate lead genes with the potential to increase biomass production.
Y1  - 2009
UR  - http://www.pnas.org/
U6  - https://doi.org/10.1073/pnas.0903478106
SN  - 0027-8424
ER  - 
TY  - JOUR
A1  - Depuydt, Stephen
A1  - Trenkamp, Sandra
A1  - Fernie, Alisdair R.
A1  - Elftieh, Samira
A1  - Renou, Jean-Pierre
A1  - Vuylsteke, Marnik
A1  - Holsters, Marcelle
A1  - Vereecke, Danny
T1  - An integrated genomics approach to define niche establishment by Rhodococcus fascians
N2  - Rhodococcus fascians is a Gram-positive phytopathogen that induces shooty hyperplasia on its hosts through the secretion of cytokinins. Global transcriptomics using microarrays combined with profiling of primary metabolites on infected Arabidopsis (Arabidopsis thaliana) plants revealed that this actinomycete modulated pathways to convert its host into a niche. The transcript data demonstrated that R. fascians leaves a very characteristic mark on Arabidopsis with a pronounced cytokinin response illustrated by the activation of cytokinin perception, signal transduction, and homeostasis. The microarray data further suggested active suppression of an oxidative burst during the R. fascians pathology, and comparison with publicly available transcript data sets implied a central role for auxin in the prevention of plant defense activation. Gene Ontology categorization of the differentially expressed genes hinted at a significant impact of infection on the primary metabolism of the host, which was confirmed by subsequent metabolite profiling. The much higher levels of sugars and amino acids in infected plants are presumably accessed by the bacteria as carbon and nitrogen sources to support epiphytic and endophytic colonization. Hexoses, accumulating from a significantly increased invertase activity, possibly inhibited the expression of photosynthesis genes and photosynthetic activity in infected leaves. Altogether, these changes are indicative of sink development in symptomatic tissues. The metabolomics data furthermore point to the possible occurrence of secondary signaling during the interaction, which might contribute to symptom development. These data are placed in the context of regulation of bacterial virulence gene expression, suppression of defense, infection phenotype, and niche establishment.
Y1  - 2009
UR  - http://www.plantphysiol.org/
U6  - https://doi.org/10.1104/pp.108.131805
SN  - 0032-0889
ER  -