TY  - JOUR
A1  - Lisdat, Fred
A1  - Dronov, Roman
A1  - Möhwald, Helmuth
A1  - Scheller, Frieder W.
A1  - Kurth, Dirk G.
T1  - Self-assembly of electro-active protein architectures on electrodes for the construction of biomimetic signal chains
N2  - The layer-by-layer adsorption technique based on the consecutive deposition of oppositely charged species is for the preparation of protein multilayers with fully electro-active protein molecules. The methodology was established with cytochrome c and the polyelectrolyte sulfonated polyaniline (PASA). The technique is also useful for the construction of bi-protein architectures confining protein-protein communication to an electrode. Following natural examples of protein complexes with defined signal transfer, cytochrome c was arranged with enzymes such as xanthine oxidase, bilirubin oxidase, laccase, and sulfite oxidase in self-assembled multilayer architectures. Thus, biomimetic signal chains from the enzyme substrate via the enzyme and cytochrome c towards the electrode can be established. Communication between proteins immobilised in multiple layers on the electrode can be achieved by in situ generation of small shuttle molecules or more advantageously by direct interprotein electron transfer. This allows the construction of new sensing electrodes, the properties of which can be tuned by the number of deposited protein layers. The mechanism of electron transfer within such protein assemblies on gold electrodes will be discussed.
Y1  - 2009
UR  - http://xlink.rsc.org/jumptojournal.cfm?journal_code=CC
U6  - https://doi.org/10.1039/B813559b
SN  - 1359-7345
ER  - 
TY  - JOUR
A1  - Kreft, Oliver
A1  - Georgieva, Radostina
A1  - Bäumler, Hans
A1  - Steup, Martin
A1  - Müller-Röber, Bernd
A1  - Sukhorukov, Gleb B.
A1  - Möhwald, Helmuth
T1  - Red blood cell templated polyelectrolyte capsules : a novel vehicle for the stable encapsulation of DNA and proteins
N2  - A novel method for the encapsulation of biomacromolecules, such as nucleic acids and proteins, into polyelectrolyte microcapsules is described. Fluorescence-labelled double-stranded DNA and human serum albumin (HSA) are used as model substances for encapsulation in hollow microcapsules templated on human erythrocytes. The encapsulation procedure involves an intermediate drying C, step. The accumulation of DNA and HSA in the capsules is observed by confocal laser scanning microscopy, UV spectroscopy, and flourimetry. The mechanism of encapsulation is discussed
Y1  - 2006
UR  - http://www3.interscience.wiley.com/cgi-bin/jhome/10003270
U6  - https://doi.org/10.1002/marc.200500777
SN  - 1022-1336
ER  - 
TY  - JOUR
A1  - Beissenhirtz, Moritz Karl
A1  - Scheller, Frieder W.
A1  - Stöcklein, Walter F. M.
A1  - Kurth, D.
A1  - Möhwald, Helmuth
A1  - Lisdat, Fred
T1  - Electroactive cytochrome c multilayers within a polyelectrolyte assembly
Y1  - 2004
ER  -