TY  - JOUR
A1  - Liu, Qinsong
A1  - Vain, Thomas
A1  - Viotti, Corrado
A1  - Doyle, Siamsa M.
A1  - Tarkowska, Danuse
A1  - Novak, Ondrej
A1  - Zipfel, Cyril
A1  - Sitbon, Folke
A1  - Robert, Stephanie
A1  - Hofius, Daniel
T1  - Vacuole integrity maintained by DUF300 proteins is required for brassinosteroid signaling regulation
JF  - Molecular plant
N2  - Brassinosteroid (BR) hormone signaling controls multiple processes during plant growth and development and is initiated at the plasma membrane through the receptor kinase BRASSINOSTEROID INSENSITIVE1 (BRI1) together with co-receptors such as BRI1-ASSOCIATED RECEPTOR KINASE1 (BAK1). BRI1 abundance is regulated by endosomal recycling and vacuolar targeting, but the role of vacuole-related proteins in BR receptor dynamics and BR responses remains elusive. Here, we show that the absence of two DUF300 domain-containing tonoplast proteins, LAZARUS1 (LAZ1) and LAZ1 HOMOLOG1 (LAZ1H1), causes vacuole morphology defects, growth inhibition, and constitutive activation of BR signaling. Intriguingly, tonoplast accumulation of BAK1 was substantially increased and appeared causally linked to enhanced BRI1 trafficking and degradation in laz1 laz1h1 plants. Since unrelated vacuole mutants exhibited normal BR responses, our findings indicate that DUF300 proteins play distinct roles in the regulation of BR signaling by maintaining vacuole integrity required to balance subcellular BAK1 pools and BR receptor distribution.
KW  - brassinosteroid signaling
KW  - vacuole integrity
KW  - DUF300 proteins
KW  - tonoplast
KW  - Arabidopsis
Y1  - 2018
U6  - https://doi.org/10.1016/j.molp.2017.12.015
SN  - 1674-2052
SN  - 1752-9867
VL  - 11
IS  - 4
SP  - 553
EP  - 567
PB  - Cell Press
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Leong, Jia Xuan
A1  - Raffeiner, Margot
A1  - Spinti, Daniela
A1  - Langin, Gautier
A1  - Franz-Wachtel, Mirita
A1  - Guzman, Andrew R.
A1  - Kim, Jung-Gun
A1  - Pandey, Pooja
A1  - Minina, Alyona E.
A1  - Macek, Boris
A1  - Hafren, Anders
A1  - Bozkurt, Tolga O.
A1  - Mudgett, Mary Beth
A1  - Börnke, Frederik
A1  - Hofius, Daniel
A1  - Uestuen, Suayib
T1  - A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component
JF  - The EMBO journal
N2  - Beyond its role in cellular homeostasis, autophagy plays anti- and promicrobial roles in host-microbe interactions, both in animals and plants. 
One prominent role of antimicrobial autophagy is to degrade intracellular pathogens or microbial molecules, in a process termed xenophagy. 
Consequently, microbes evolved mechanisms to hijack or modulate autophagy to escape elimination. 
Although well-described in animals, the extent to which xenophagy contributes to plant-bacteria interactions remains unknown. 

Here, we provide evidence that Xanthomonas campestris pv. vesicatoria (Xcv) suppresses host autophagy by utilizing type-III effector XopL. XopL interacts with and degrades the autophagy component SH3P2 via its E3 ligase activity to promote infection. 
Intriguingly, XopL is targeted for degradation by defense-related selective autophagy mediated by NBR1/Joka2, revealing a complex antagonistic interplay between XopL and the host autophagy machinery. 

Our results implicate plant antimicrobial autophagy in the depletion of a bacterial virulence factor and unravel an unprecedented pathogen strategy to counteract defense-related autophagy in plant-bacteria interactions.
KW  - autophagy
KW  - effectors
KW  - immunity
KW  - ubiquitination
KW  - xenophagy
Y1  - 2022
U6  - https://doi.org/10.15252/embj.2021110352
SN  - 0261-4189
SN  - 1460-2075
VL  - 41
IS  - 13
PB  - Wiley
CY  - Hoboken
ER  -