TY  - JOUR
A1  - Holzlöhner, Pamela
A1  - Butze, Monique
A1  - Maier, Natalia
A1  - Hebel, Nicole
A1  - Schliebs, Erik
A1  - Micheel, Burkhard
A1  - Fuener, Jonas
A1  - Heidicke, Gabriele
A1  - Hanack, Katja
T1  - Generation of murine monoclonal antibodies with specificity against conventional camelid IgG1 and heavy-chain only IgG2/3
JF  - Veterinary Immunology and Immunopathology
N2  - Camelids possess antibodies with a conventional four-chain structure consisting of two heavy and two light chains (of subclass IgG1) but further they also generate heavy-chain only antibodies (of subclass IgG2 and 3) which are fully functional in antigen binding. In this study subclass-specific murine monoclonal antibodies specific to conventional camelid IgG1 and heavy-chain only IgG2/3 were generated and validated for the use as potent secondary detection reagents. The monoclonal antibodies are able to differentiate between all camelid IgGs, conventional four-chain camelid antibodies (of subclass IgG1) and exclusively heavy chain-only antibodies (of subclasses IgG2 and IgG3). Further these antibodies were used to detect specific immune responses after vaccination of Camelids against bovine corona- and rotavirus strains and different E.coli. and Clostridia - antigens and to identify Erysipelothrix rhusiopathiae infected animals within a herd. The described antibodies are suitable as new secondary agents for the detection of different camelid subclasses and the validation of camelid immune reactions.
KW  - Camelid antibodies
KW  - Heavy-chain only antibodies
KW  - Monoclonal antibodies
KW  - Secondary antibodies
KW  - Vaccination
KW  - Disease monitoring
Y1  - 2018
U6  - https://doi.org/10.1016/j.vetimm.2018.01.006
SN  - 0165-2427
SN  - 1873-2534
VL  - 197
SP  - 1
EP  - 6
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - GEN
A1  - Hanack, Katja
A1  - Schloer, Anja
A1  - Holzloehner, Pamela
A1  - Listek, Martin
A1  - Bauer, Cindy
A1  - Butze, Monique
A1  - Micheel, Burkhard
A1  - Hentschel, Christian
A1  - Sowa, Mandy
A1  - Roggenbuck, Dirk
A1  - Schierack, Peter
A1  - Fuener, Jonas
A1  - Schliebs, Erik
A1  - Goihl, Alexander
A1  - Reinhold, Dirk
T1  - Camelid nanobodies specific to human pancreatic glycoprotein 2
T2  - The journal of immunology
N2  - Pancreatic secretory zymogen-granule membrane glycoprotein 2 (GP2) has been identified to be a major autoantigenic target in Crohn’s disease patients. It was discussed recently that a long and a short isoform of GP2 exists whereas the short isoform is often detected by GP2-specific autoantibodies. In the outcome of inflammatory bowel diseases, these GP2-specific autoantibodies are discussed as new serological markers for diagnosis and therapeutic monitoring. To investigate this further, camelid nanobodies were generated by phage display and selected against the short isoform of GP2 in order to isolate specific tools for the discrimination of both isoforms. Nanobodies are single domain antibodies derived from camelid heavy chain only antibodies and characterized by a high stability and solubility. The selected candidates were expressed, purified and validated regarding their binding properties in different enzyme-linked immunosorbent assays formats, immunofluorescence, immunohistochemistry and surface plasmon resonance spectroscopy. Four different nanobodies could be selected whereof three recognize the short isoform of GP2 very specifically and one nanobody showed a high binding capacity for both isoforms. The KD values measured for all nanobodies were between 1.3 nM and 2.3 pM indicating highly specific binders suitable for the application as diagnostic tool in inflammatory bowel disease.
Y1  - 2016
SN  - 0022-1767
SN  - 1550-6606
VL  - 196
SP  - 313
EP  - 328
PB  - American Assoc. of Immunologists
CY  - Bethesda
ER  -