TY  - JOUR
A1  - Bistolas, Nikitas
A1  - Christenson, A.
A1  - Ruzgas, T.
A1  - Jung, Christiane
A1  - Scheller, Frieder W.
A1  - Wollenberger, Ursula
T1  - Spectroelectrochemistry of cytochrome P450cam
N2  - The spectroelectrochemistry of camphor-bound cytochrome P450cam (P450cam) using gold electrodes is described. The electrodes were modified with either 4,4'-dithiodipyridin or sodium dithionite. Electrolysis of P450cam was carried out when the enzyme was in solution, while at the same time UV visible absorption spectra were recorded. Reversible oxidation and reduction could be observed with both 4,4'-dithiodipyridin and dithionite modified electrodes. A formal potential (E-0') of -373 mV vs Ag/AgCl 1 M KCl was determined. The spectra of P450cam complexed with either carbon monoxide or metyrapone, both being inhibitors of P450 catalysis, clearly indicated that the protein retained its native state in the electrochemical cell during electrolysis. (C) 2003 Elsevier Inc. All rights reserved
Y1  - 2004
ER  -