TY  - JOUR
A1  - Yan, Wenhao
A1  - Chen, Dijun
A1  - Schumacher, Julia
A1  - Durantini, Diego
A1  - Engelhorn, Julia
A1  - Chen, Ming
A1  - Carles, Cristel C.
A1  - Kaufmann, Kerstin
T1  - Dynamic control of enhancer activity drives stage-specific gene expression during flower morphogenesis
JF  - Nature Communications
N2  - Enhancers are critical for developmental stage-specific gene expression, but their dynamic regulation in plants remains poorly understood. Here we compare genome-wide localization of H3K27ac, chromatin accessibility and transcriptomic changes during flower development in Arabidopsis. H3K27ac prevalently marks promoter-proximal regions, suggesting that H3K27ac is not a hallmark for enhancers in Arabidopsis. We provide computational and experimental evidence to confirm that distal DNase. hypersensitive sites are predictive of enhancers. The predicted enhancers are highly stage-specific across flower development, significantly associated with SNPs for flowering-related phenotypes, and conserved across crucifer species. Through the integration of genome-wide transcription factor (TF) binding datasets, we find that floral master regulators and stage-specific TFs are largely enriched at developmentally dynamic enhancers. Finally, we show that enhancer clusters and intronic enhancers significantly associate with stage-specific gene regulation by floral master TFs. Our study provides insights into the functional flexibility of enhancers during plant development, as well as hints to annotate plant enhancers.
Y1  - 2019
U6  - https://doi.org/10.1038/s41467-019-09513-2
SN  - 2041-1723
VL  - 10
PB  - Nature Publ. Group
CY  - London
ER  -