TY - THES A1 - Kamprath, Martin T1 - A microfoundations perspectives on fresight and business models BT - Competence development and business model design in times of digital industry transformation Y1 - 2014 ER - TY - THES A1 - Supaporn, Worakrit T1 - Categorical equivalence of clones Y1 - 2014 ER - TY - JOUR A1 - Hafer, Jörg A1 - Kiy, Alexander A1 - Lucke, Ulrike T1 - Moodle & Co. auf dem Weg zur Personal Learning Environment JF - eleed N2 - Ausgehend von der typischen IT‐Infrastruktur für E‐Learning an Hochschulen auf der einen Seite sowie vom bisherigen Stand der Forschung zu Personal Learning Environments (PLEs) auf der anderen Seite zeigt dieser Beitrag auf, wie bestehende Werkzeuge bzw. Dienste zusammengeführt und für die Anforderungen der modernen, rechnergestützten Präsenzlehre aufbereitet werden können. Für diesen interdisziplinären Entwicklungsprozess bieten sowohl klassische Softwareentwicklungsverfahren als auch bestehende PLE‐Modelle wenig Hilfestellung an. Der Beitrag beschreibt die in einem campusweiten Projekt an der Universität Potsdam verfolgten Ansätze und die damit erzielten Ergebnisse. Dafür werden zunächst typische Lehr‐/Lern‐bzw. Kommunikations‐Szenarien identifiziert, aus denen Anforderungen an eine unterstützende Plattform abgeleitet werden. Dies führt zu einer umfassenden Sammlung zu berücksichtigender Dienste und deren Funktionen, die gemäß den Spezifika ihrer Nutzung in ein Gesamtsystem zu integrieren sind. Auf dieser Basis werden grundsätzliche Integrationsansätze und technische Details dieses Mash‐Ups in einer Gesamtschau aller relevanten Dienste betrachtet und in eine integrierende Systemarchitektur überführt. Deren konkrete Realisierung mit Hilfe der Portal‐Technologie Liferay wird dargestellt, wobei die eingangs definierten Szenarien aufgegriffen und exemplarisch vorgestellt werden. Ergänzende Anpassungen im Sinne einer personalisierbaren bzw. adaptiven Lern‐(und Arbeits‐)Umgebung werden ebenfalls unterstützt und kurz aufgezeigt. Y1 - 2014 UR - https://eleed.campussource.de/archive/10/4085 SN - 1860-7470 VL - 2014 IS - 10 ER - TY - JOUR A1 - Boggio, Jose M. Chavez A1 - Bodenmueller, D. A1 - Fremberg, T. A1 - Haynes, R. A1 - Roth, Martin M. A1 - Eisermann, R. A1 - Lisker, M. A1 - Zimmermann, L. A1 - Boehm, Michael T1 - Dispersion engineered silicon nitride waveguides by geometrical and refractive-index optimization JF - Journal of the Optical Society of America : B, Optical physics N2 - Dispersion engineering in silicon nitride (SiXNY) waveguides is investigated through the optimization of the waveguide transversal dimensions and refractive indices in a multicladding arrangement. Ultraflat dispersion of -84.0 +/- 0.5 ps/nm/km between 1700 and 2440 nm and 1.5 +/- 3 ps/nm/km between 1670 and 2500 nm is numerically demonstrated. It is shown that typical refractive index fluctuations as well as dimension fluctuations during fabrication of the SiXNY waveguides are a limitation for obtaining ultraflat dispersion profiles. Single- and multicladding waveguides are fabricated and their dispersion profiles measured (over nearly 1000 nm) using a low-coherence frequency domain interferometric technique. By appropriate thickness optimization, the zero-dispersion wavelength is tuned over a large spectral range in single-and multicladding waveguides with small refractive index contrast (3%). A flat dispersion profile with +/- 3.2 ps/nm/km variation over 500 nm is obtained in a multicladding waveguide fabricated with a refractive index contrast of 37%. Finally, we generate a nearly three-octave supercontinuum in this dispersion flattened multicladding SiXNY waveguide. (C) 2014 Optical Society of America Y1 - 2014 U6 - https://doi.org/10.1364/JOSAB.31.002846 SN - 0740-3224 SN - 1520-8540 VL - 31 IS - 11 SP - 2846 EP - 2857 PB - Optical Society of America CY - Washington ER - TY - JOUR A1 - Schmaelzlin, Elmar A1 - Moralejo, Benito A1 - Rutowska, Monika A1 - Monreal-Ibero, Ana A1 - Sandin, Christer A1 - Tarcea, Nicolae A1 - Popp, Juergen A1 - Roth, Martin M. T1 - Raman imaging with a fiber-coupled multichannel spectrograph JF - Sensors N2 - Until now, spatially resolved Raman Spectroscopy has required to scan a sample under investigation in a time-consuming step-by-step procedure. Here, we present a technique that allows the capture of an entire Raman image with only one single exposure. The Raman scattering arising from the sample was collected with a fiber-coupled high-performance astronomy spectrograph. The probe head consisting of an array of 20 x 20 multimode fibers was linked to the camera port of a microscope. To demonstrate the high potential of this new concept, Raman images of reference samples were recorded. Entire chemical maps were received without the need for a scanning procedure. KW - multichannel Raman spectroscopy KW - astronomy spectrograph KW - optical fiber bundle KW - Raman imaging Y1 - 2014 U6 - https://doi.org/10.3390/s141121968 SN - 1424-8220 VL - 14 IS - 11 SP - 21968 EP - 21980 PB - MDPI CY - Basel ER - TY - JOUR A1 - Beisheim, Marianne A1 - Liese, Andrea Margit T1 - Summing up : key findings and avenues for future research Y1 - 2014 SN - 978-1-137-35925-0 ER - TY - BOOK A1 - Hafer, Jörg A1 - Henze, Luise A1 - Matthé, Frederic A1 - Schumann, Marlen T1 - Bestandsaufnahme E-Learning an der Universität Potsdam N2 - Der vorliegende Text gibt eine Bestandserhebung der bisher stattgefundenen Aktivitäten im E-Learning an der Universität Potsdam wieder, andererseits dient er auch dazu, Potenziale zu sichten und in einem nächstem Schritt daraus Ideen und Vorschläge für eine hochschulweite E-Learning-Strategie abzuleiten. Zielsetzung der Bestandsaufnahme ist es, die relevanten Informationen darzustellen, über den Platz der Universität Potsdam in der hochschulischen E-Learning-Landschaft zu orientieren und den Stand der Entwicklung zu bewerten. T3 - Potsdamer Beiträge zum E-Learning - 1 KW - E-Learning KW - Digitale Medien KW - Hochschule KW - Lehre KW - Studium Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-70670 SN - 2199-6520 IS - 1 ER - TY - THES A1 - Faber, Eike T1 - Von Ulfila bis Rekkared BT - die Goten und ihr Christentum T2 - Potsdamer altertumswissenschaftliche Beiträge ; Bd. 51 N2 - Für etwa 200 Jahre, vom Ende des 4. Jahrhunderts bis 589 n. Chr., gehören die Westgoten einem von der Orthodoxie der Reichskirche abweichenden christlichen Bekenntnis an. Auf dem 3. Konzil von Toledo beendet König Rekkared diesen Zustand religiöser Alterität durch die Konversion zum Katholizismus. Die antiken Berichte zeichnen nur vordergründig ein kohärentes Bild des Christentums der Goten. Dagegen weist Eike Faber Widersprüche und Fehler in der Überlieferung nach und bietet eine Reihe neuer Interpretationen an. Die genauere Betrachtung vermag dabei sowohl den dogmatischen Gehalt des Christentums der Goten schärfer zu konturieren als auch die Beweggründe für die Annahme des neuen Glaubens herauszustellen und die traditionelle Datierung dieses Vorgangs zu widerlegen. Schließlich wird deutlich, welche Funktion das fortgesetzte Festhalten an einer demonstrativ anderen religiösen Überzeugung für die Goten hatte. Prägnante Wegmarken der gotischen Geschichte, wie die Bibelübersetzung Ulfilas, die Eroberung und Plünderung Roms 410 n. Chr. oder die demonstrative Aufgabe der religiösen Differenz durch König Rekkared, werden erst durch diesen neuen, umfassenden Kontext verständlich. Y1 - 2014 SN - 978-3-515-10929-1 SN - 978-3-515-10926-0 U6 - https://doi.org/10.25162/9783515109291 PB - Steiner CY - Stuttgart ER - TY - JOUR A1 - Sultanow, Eldar A1 - Weber, Edzard T1 - Prozessleitstände für verteilte und nichtplanbare Organisationsprozesse BT - ein Modell für globales Kollaborations- und Wissensmanagement JF - Handbuch prozessorientiertes Wissensmanagement Y1 - 2014 SN - 978-3-95545-026-7 SP - 335 EP - 344 PB - GITO CY - Berlin ER - TY - JOUR A1 - Henke, Klaus-Dirk A1 - Podtschaske, Beatrice T1 - Chancen und Risiken genossenschaftlicher Leistungserbringung im Gesundheitssektor JF - KWI-Schriften N2 - Klaus-Dirk Henke, em. Lehrstuhlinhaber für Öffentliche Finanzen und Gesundheitsökonomie am Institut für Volkswirtschaftslehre und Wirtschaftsrecht der TU Berlin, behandelt, ausgehend von der derzeitigen Leistungserbringung im Gesundheitssektor, Chancen und Risiken der Leistungserbringung durch Genossenschaften. Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-71045 SN - 1867-951X SN - 1867-9528 IS - 8 SP - 63 EP - 71 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Wandersleb, Michael T1 - IT-Genossenschaften im digitalen Rathaus BT - ein Praxisbericht JF - KWI-Schriften N2 - Matthias Klipp, Beigeordneter für Stadtentwicklung und Bauen der Stadtverwaltung Potsdam, diskutiert die Rolle von Genossenschaften als Bausteine kommunaler Wohnungspolitik. Am Beispiel der Landeshauptstadt Potsdam zeigt er Potenziale von Wohnungsbaugenossenschaften für die Sicherung einer attraktiven und ausreichenden Wohnungsversorgung für die BürgerInnen auf. Er resümiert, dass sich die Strategien der Stadt für Neubau und Bestand auch in Kooperation mit Wohnungsgenossenschaften gerade auch bei der sozialen Wohnraumversorgung für die Bevölkerung bewährt haben. Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-71068 SN - 1867-951X SN - 1867-9528 IS - 8 SP - 81 EP - 92 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Wieg, Andreas T1 - Krisenerprobung und Krisenresistenz des genossenschaftlichen Ordnungsmodells JF - KWI-Schriften N2 - Andreas Wieg, Abteilungsleiter Vorstandsstab des „Deutschen Genossenschafts- und Raiffeisenverband e. V.“, befasst sich in seinem Beitrag mit der Krisenerprobung und Krisenresistenz des genossenschaftlichen Ordnungsmodells. Dabei geht er auf die genossenschaftliche Organisationsstruktur und auf die Genossenschaftsarten ein. Er beschreibt Möglichkeiten von Genossenschaftsgründungen und geht abschließend auf die Nachhaltigkeit neuer Genossenschaften ein. Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-71005 SN - 1867-951X SN - 1867-9528 IS - 8 SP - 23 EP - 31 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Amschler, Helmut T1 - „NEW“ – Neue Energien West eG und Bürger-Energiegenossenschaft West eG BT - zwei Genossenschaften – ein Ziel JF - KWI-Schriften N2 - Helmut Amschler, Vorstand der Stadtwerke Grafenwöhr, stellt in seinem Beitrag 2 konkrete Genossenschaften – die Neue Energien West eG und die Bürger-Energiegenossenschaft West eG vor. Dabei diskutiert er u. a. Kriterien wie Bürgerbeteiligung und Transparenz. Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-71037 SN - 1867-951X SN - 1867-9528 IS - 8 SP - 57 EP - 62 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Klipp, Matthias T1 - Genossenschaften BT - Bausteine kommunaler Wohnungsmarktpolitik JF - KWI-Schriften N2 - Matthias Klipp, Beigeordneter für Stadtentwicklung und Bauen der Stadtverwaltung Potsdam, diskutiert die Rolle von Genossenschaften als Bausteine kommunaler Wohnungspolitik. Am Beispiel der Landeshauptstadt Potsdam zeigt er Potenziale von Wohnungsbaugenossenschaften für die Sicherung einer attraktiven und ausreichenden Wohnungsversorgung für die BürgerInnen auf. Er resümiert, dass sich die Strategien der Stadt für Neubau und Bestand auch in Kooperation mit Wohnungsgenossenschaften gerade auch bei der sozialen Wohnraumversorgung für die Bevölkerung bewährt haben. Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-71058 SN - 1867-951X SN - 1867-9528 IS - 8 SP - 73 EP - 80 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - CHAP A1 - Knoth, Alexander Henning A1 - Kiy, Alexander T1 - (Self-)confident through the introductory study phase with the Reflect App BT - (Self-)confident through the introductory study phase with the Reflect App T2 - CEUR Workshop Proceedings T2 - Selbst-bewusst durch die Studieneingangsphase mit der Reflect-App Y1 - 2014 UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-84921875162&partnerID=MN8TOARS SN - 1613-0073 IS - 1227 SP - 172 EP - 179 PB - CEUR-WS CY - Freiburg ER - TY - GEN A1 - Arni, Patrick A1 - Caliendo, Marco A1 - Künn, Steffen A1 - Zimmermann, Klaus F. T1 - The IZA evaluation dataset survey BT - a scientific use file T2 - Postprints der Universität Potsdam : Wirtschafts- und Sozialwissenschaftliche Reihe N2 - This reference paper describes the sampling and contents of the IZA Evaluation Dataset Survey and outlines its vast potential for research in labor economics. The data have been part of a unique IZA project to connect administrative data from the German Federal Employment Agency with innovative survey data to study the out-mobility of individuals to work. This study makes the survey available to the research community as a Scientific Use File by explaining the development, structure, and access to the data. Furthermore, it also summarizes previous findings with the survey data. T3 - Zweitveröffentlichungen der Universität Potsdam : Wirtschafts- und Sozialwissenschaftliche Reihe - 122 KW - survey data KW - scientific use file KW - labor market policies KW - evaluation KW - migration KW - ethnicity KW - attitudes KW - behavior KW - skills Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-435204 SN - 1867-5808 IS - 122 ER - TY - JOUR A1 - Vladova, Gergana A1 - Bahrs, Julian T1 - Wissenstransfer als Mittel der internen Unternehmenskommunikation BT - eine explorative Untersuchung in neun internationalen Unternehmen JF - Handbuch prozessorientiertes Wissensmanagment Y1 - 2014 SN - 978-3-95545-026-7 SP - 37 EP - 47 PB - GITO CY - Berlin ER - TY - JOUR A1 - Apelojg, Benjamin ED - Banse, Gerhard ED - Grimmeis, Hermann T1 - Innovationen-Kreativität-Schule BT - eine Betrachtung verschiedener Innovationskonzepte aus der Sicht von Schule JF - Wissenschaft - Innovation- Technologie KW - Innovationen KW - Schule KW - Kreativität Y1 - 2014 SN - 978-3864640537 VL - 37 SP - 325 EP - 340 PB - Trafo-Verlag CY - Berlin ER - TY - JOUR A1 - Hohenstein, Sven A1 - Kliegl, Reinhold T1 - Semantic preview benefit during reading JF - Journal of experimental psychology : Learning, memory, and cognition N2 - Word features in parafoveal vision influence eye movements during reading. The question of whether readers extract semantic information from parafoveal words was studied in 3 experiments by using a gaze-contingent display change technique. Subjects read German sentences containing 1 of several preview words that were replaced by a target word during the saccade to the preview (boundary paradigm). In the 1st experiment the preview word was semantically related or unrelated to the target. Fixation durations on the target were shorter for semantically related than unrelated previews, consistent with a semantic preview benefit. In the 2nd experiment, half the sentences were presented following the rules of German spelling (i.e., previews and targets were printed with an initial capital letter), and the other half were presented completely in lowercase. A semantic preview benefit was obtained under both conditions. In the 3rd experiment, we introduced 2 further preview conditions, an identical word and a pronounceable nonword, while also manipulating the text contrast. Whereas the contrast had negligible effects, fixation durations on the target were reliably different for all 4 types of preview. Semantic preview benefits were greater for pretarget fixations closer to the boundary (large preview space) and, although not as consistently, for long pretarget fixation durations (long preview time). The results constrain theoretical proposals about eye movement control in reading. KW - eye movements KW - reading KW - semantic preview benefit KW - parafoveal processing KW - display change awareness Y1 - 2014 U6 - https://doi.org/10.1037/a0033670 SN - 0278-7393 SN - 1939-1285 VL - 40 IS - 1 SP - 166 EP - 190 PB - American Psychological Association CY - Washington ER - TY - JOUR A1 - Badalyan, Artavazd A1 - Dierich, Marlen A1 - Stiba, Konstanze A1 - Schwuchow, Viola A1 - Leimkühler, Silke A1 - Wollenberger, Ulla T1 - Electrical wiring of the aldehyde oxidoreductase PaoABC with a polymer containing osmium redox centers BT - biosensors for benzaldehyde and GABA JF - Biosensors N2 - Biosensors for the detection of benzaldehyde and g-aminobutyric acid (GABA) are reported using aldehyde oxidoreductase PaoABC from Escherichia coli immobilized in a polymer containing bound low potential osmium redox complexes. The electrically connected enzyme already electrooxidizes benzaldehyde at potentials below −0.15 V (vs. Ag|AgCl, 1 M KCl). The pH-dependence of benzaldehyde oxidation can be strongly influenced by the ionic strength. The effect is similar with the soluble osmium redox complex and therefore indicates a clear electrostatic effect on the bioelectrocatalytic efficiency of PaoABC in the osmium containing redox polymer. At lower ionic strength, the pH-optimum is high and can be switched to low pH-values at high ionic strength. This offers biosensing at high and low pH-values. A “reagentless” biosensor has been formed with enzyme wired onto a screen-printed electrode in a flow cell device. The response time to addition of benzaldehyde is 30 s, and the measuring range is between 10–150 µM and the detection limit of 5 µM (signal to noise ratio 3:1) of benzaldehyde. The relative standard deviation in a series (n = 13) for 200 µM benzaldehyde is 1.9%. For the biosensor, a response to succinic semialdehyde was also identified. Based on this response and the ability to work at high pH a biosensor for GABA is proposed by coimmobilizing GABA-aminotransferase (GABA-T) and PaoABC in the osmium containing redox polymer. KW - redox polymer KW - aldehyde oxidoreductase KW - ionic strength KW - benzaldehyde KW - GABA KW - biosensor Y1 - 2014 U6 - https://doi.org/10.3390/bios4040403 VL - 4 IS - 4 SP - 403 EP - 421 PB - MDPI CY - Basel ER - TY - CHAP A1 - Wochatz, Monique A1 - Kopinski, Stephan A1 - Engel, Tilman A1 - Müller, Steffen A1 - Mayer, Frank T1 - Flexion-extension ratio of trunk peak torque measures and antagonistic activity in males and females T2 - Medicine and science in sports and exercise : official journal of the American College of Sports Medicine Y1 - 2014 SN - 0195-9131 SN - 1530-0315 VL - 46 IS - 5 SP - 148 EP - 148 PB - Lippincott Williams & Wilkins CY - Philadelphia ER - TY - JOUR A1 - George, Wolfgang T1 - Regionalentwicklung durch genossenschaftlich organisiertes Engagement JF - KWI-Schriften N2 - Wolfgang George, Honorarprofessor der TH Mittelhessen und Vorstand der Andramedos eG, diskutiert ausgehend von seiner Definition der Regionalität Potenziale genossenschaftlicher Lösungen am Beispiel der Regionalen Energieversorgung (REV). Er resümiert, dass das Potenzial genossenschaftlicher Lösungen bis heute in diesem Feld nicht annähernd ausgeschöpft sei. Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-71013 SN - 1867-951X SN - 1867-9528 IS - 8 SP - 33 EP - 48 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Fritzsch, Claire A1 - Wang, Jing A1 - dos Santos, Luara Ferreira A1 - Mauritz, Karl-Heinz A1 - Brunetti, Maddalena A1 - Dohle, Christian T1 - Different effects of the mirror illusion on motor and somatosensory processing JF - Restorative neurology and neuroscience N2 - Purpose: Mirror therapy can improve motor and sensory functions, but effects of the mirror illusion on primary motor and somatosensory cortex could not be established consistently. Methods: Fifteen right handed healthy volunteers performed or observed a finger-thumb opposition task. Cerebral activations during normal movement (NOR), mirrored movement (MIR) and movement observation (OBS) by means of a video chain were recorded with functional magnetic resonance imaging (fMRI). Activation sizes in movement > static conditions were identified using SPM8 (p < 0.001, unc.) and attributed to predefined areas employing the Anatomy toolbox 1.8. Laterality indices for the responsive areas were calculated on the basis of the number of activated voxels. Results: Relevant bilateral BOLD responses were found in primary motor (M1) and somatosensory (S1 - BA 2, 3b and 3a) cortex, premotor and parietal areas and V5. When comparing MIR to NOR, no significant change of contralateral activation in M1 was found, but clearly at S1 with differences between hands. Conclusion: The mirror illusion does not elicit immediate changes in motor areas, yet there is a direct effect on somatosensory areas, especially for left hand movements. These results suggest different effects of mirror therapy on processing and rehabilitation of motor and sensory function. KW - Movement KW - mirror KW - laterality KW - stroke KW - sensorimotor cortex Y1 - 2014 U6 - https://doi.org/10.3233/RNN-130343 SN - 0922-6028 SN - 1878-3627 VL - 32 IS - 2 SP - 269 EP - 280 PB - IOS Press CY - Amsterdam ER - TY - JOUR A1 - Wyrwa, Ulrich T1 - For the Hundredth nothing New German Language New Releases to the First World War (Part I) JF - Deutsche Zeitschrift für Geschichtswissenschaft Y1 - 2014 SN - 0044-2828 SN - 1618-0372 VL - 62 IS - 11 SP - 921 EP - 940 PB - Metropol-Verl. CY - Berlin ER - TY - JOUR A1 - Crome, Erhard T1 - Deutschland und die USA : eine geopolitische Perspektive Y1 - 2014 ER - TY - JOUR A1 - Röder, Katrin T1 - Reparative reading, post-structuralist Hermeneutics and T. S. Eliot's four quartets JF - Anglia : journal of English philology N2 - This essay approaches T. S. Eliot's Four Quartets (1935-1942) from the perspectives of Eve Kosofsky Sedgwick's critical practice of reparative reading and of Paul Ricoeur's poststructuralist hermeneutics. It demonstrates that Sedgwick's and Ricoeur's approaches can be productively combined to investigate hermeneutic processes in which the textual energy of a dissemination of meaning is redirected by a reparative or integrative impulse. In Four Quartets, this impetus induces the creation of semantic innovation through a violation of semantic pertinence, that is, through novel, tensional and provisional connections between formerly separate textual elements and semantic units. Y1 - 2014 U6 - https://doi.org/10.1515/anglia-2014-0004 SN - 0340-5222 SN - 1865-8938 VL - 132 IS - 1 SP - 58 EP - 77 PB - De Gruyter CY - Berlin ER - TY - JOUR A1 - Dolgener, Nicola A1 - Freudenberger, L. A1 - Schneeweiss, N. A1 - Ibisch, P. L. A1 - Tiedemann, Ralph T1 - Projecting current and potential future distribution of the Fire-bellied toad Bombina bombina under climate change in north-eastern Germany JF - Regional environmental change N2 - Environmental change is likely to have a strong impact on biodiversity, and many species may shift their distribution in response. In this study, we aimed at projecting the availability of suitable habitat for an endangered amphibian species, the Fire-bellied toad Bombina bombina, in Brandenburg (north-eastern Germany). We modelled a potential habitat distribution map based on (1) a database with 10,581 presence records for Bombina from the years 1990 to 2009, (2) current estimates for ecogeographical variables (EGVs) and (3) the future projection of these EGVs according to the statistical regional model, respectively, the soil and water integrated model, applying the maximum entropy approach (Maxent). By comparing current and potential future distributions, we evaluated the projected change in distribution of suitable habitats and identified the environmental variables most associated with habitat suitability that turned out to be climatic variables related to the hydrological cycle. Under the applied scenario, our results indicate increasing habitat suitability in many areas and an extended range of suitable habitats. However, even if the environmental conditions in Brandenburg may change as predicted, it is questionable whether the Fire-bellied toad will truly benefit, as dispersal abilities of amphibian species are limited and strongly influenced by anthropogenic disturbances, that is, intensive agriculture, habitat destruction and fragmentation. Furthermore, agronomic pressure is likely to increase on productive areas with fertile soils and high water retention capacities, indeed those areas suitable for B. bombina. All these changes may affect temporary pond hydrology as well as the reproductive success and breeding phenology of toads. KW - Brandenburg KW - Climate change KW - Distribution modelling KW - Endangered species KW - Fire-bellied toad KW - Maxent Y1 - 2014 U6 - https://doi.org/10.1007/s10113-013-0468-9 SN - 1436-3798 SN - 1436-378X VL - 14 IS - 3 SP - 1063 EP - 1072 PB - Springer CY - Heidelberg ER - TY - JOUR A1 - Dolgener, N. A1 - Freudenberger, L. A1 - Schluck, M. A1 - Schneeweiss, N. A1 - Ibisch, P. L. A1 - Tiedemann, Ralph T1 - Environmental niche factor analysis (ENFA) relates environmental parameters to abundance and genetic diversity in an endangered amphibian, the fire-bellied-toad (Bombina bombina) JF - Conservation genetics N2 - Increasing attempts are made to understand the factors responsible for both the demographic and genetic depletion in amphibian populations. Landscape genetics aims at a spatially explicit correlation of genetic population parameters to landscape features. Using data from the endangered fire-bellied toad Bombina bombina in Brandenburg (Northeastern Germany), we performed an environmental niche factor analysis (ENFA), relating demographic (abundance) and genetic (diversity at 17 microsatellite loci and partial sequences of the mitochondrial control region in 434 individuals from 16 populations) parameters to ecological and anthropogenic variables such as temperature, precipitation, soil wetness, water runoff, vegetation density, and road/traffic impact. We found significant correlations between road disturbance and observed heterozygosity and between soil wetness and mitochondrial diversity. As the influences of the environmental variables can differ between different indicators for genetic diversity, population size and abundance data, our ENFA-based landscape genetics approach allows us to put various aspects of long- versus short term effective population size and genetic connectivity into an ecological and spatially explicit context, enabling potentially even forecast assessment under future environmental scenarios. KW - Distribution modelling KW - ENFA KW - Genetic diversity KW - Fire-bellied toad KW - Landscape genetics KW - Road disturbance Y1 - 2014 U6 - https://doi.org/10.1007/s10592-013-0517-4 SN - 1566-0621 SN - 1572-9737 VL - 15 IS - 1 SP - 11 EP - 21 PB - Springer CY - Dordrecht ER - TY - JOUR A1 - Fenn, Monika T1 - Konstruktivistisches Geschichtsverständnis im Unterricht fördern : Studierende ändern ihr Lehrverhalten von einseitig instruktional in problemorientiert Y1 - 2014 SN - 978-3- 0355-0022-6 ER - TY - JOUR A1 - Fenn, Monika A1 - Brandt, Christopher Georg T1 - Time-Travel-Treasures BT - eine Mobile-Learning-App zur Erkundung des historischen Altlandsberg JF - Tür an Tür und Haus an Haus Nachbarschaften in der historischen Stadt Themenbuch 2014 Y1 - 2014 SN - 978-3-00-047888-8 SP - 76 EP - 79 PB - Arbeitsgemeinschaft Städte mit historischen Stadkernen des Landes Brandenburg CY - Potsdam ER - TY - JOUR A1 - Tiedemann, Ralph A1 - Schneider, Anja R. R. A1 - Havenstein, Katja A1 - Blanck, Torsten A1 - Meier, Elmar A1 - Raffel, Martina A1 - Zwartepoorte, Henk A1 - Plath, Martin T1 - New microsatellite markers allow high-resolution taxon delimitation in critically endangered Asian box turtles, genus Cuora JF - Salamandra : German journal of herpetology N2 - We isolated and characterized 16 new di- and tetranudeotide microsatellite markers for the critically endangered Asian box turtle genus Cuora, focusing on the "Cuora trifasciata" species complex. The new markers were then used to analyse genetic variability and divergence amongst five described species within this complex, namely C. aurocapitata (n = 18), C. cyclornata (n = 31), C. pani (n = 6), C. trifasciata (n = 58), and C. zhoui (n = 7). Our results support the view that all five species represent valid taxa. Within two species (C. trifasciata and C. cyclornata), two distinct morphotypes were corroborated by microsatellite divergence. For three individuals, morphologically identified as being of hybrid origin, the hybrid status was confirmed by our genetic analysis. Our results confirm the controversial species (Cuora aurocapitata, C. cyclornata) and subspecies/morphotypes (C. cyclornata meieri, C. trifasciata cf. trifasciata) to be genetically distinct, which has critical implications for conservation strategies. KW - Testudines KW - Cuora KW - box turtles KW - captive breeding KW - conservation units KW - microsatellites Y1 - 2014 SN - 0036-3375 VL - 50 IS - 3 SP - 139 EP - 146 PB - Deutsche Gesellschaft für Herpetologie und Terrarienkunde CY - Darmstadt ER - TY - THES A1 - Orgis, Thomas T1 - Unstetige Galerkin-Diskretisierung niedriger Ordnung in einem atmosphärischen Multiskalenmodell T1 - Low-order discontinuous Galerkin discretization in an atmospheric multi-scale model N2 - Die Dynamik der Atmosphäre der Erde umfasst einen Bereich von mikrophysikalischer Turbulenz über konvektive Prozesse und Wolkenbildung bis zu planetaren Wellenmustern. Für Wettervorhersage und zur Betrachtung des Klimas über Jahrzehnte und Jahrhunderte ist diese Gegenstand der Modellierung mit numerischen Verfahren. Mit voranschreitender Entwicklung der Rechentechnik sind Neuentwicklungen der dynamischen Kerne von Klimamodellen, die mit der feiner werdenden Auflösung auch entsprechende Prozesse auflösen können, notwendig. Der dynamische Kern eines Modells besteht in der Umsetzung (Diskretisierung) der grundlegenden dynamischen Gleichungen für die Entwicklung von Masse, Energie und Impuls, so dass sie mit Computern numerisch gelöst werden können. Die vorliegende Arbeit untersucht die Eignung eines unstetigen Galerkin-Verfahrens niedriger Ordnung für atmosphärische Anwendungen. Diese Eignung für Gleichungen mit Wirkungen von externen Kräften wie Erdanziehungskraft und Corioliskraft ist aus der Theorie nicht selbstverständlich. Es werden nötige Anpassungen beschrieben, die das Verfahren stabilisieren, ohne sogenannte „slope limiter” einzusetzen. Für das unmodifizierte Verfahren wird belegt, dass es nicht geeignet ist, atmosphärische Gleichgewichte stabil darzustellen. Das entwickelte stabilisierte Modell reproduziert eine Reihe von Standard-Testfällen der atmosphärischen Dynamik mit Euler- und Flachwassergleichungen in einem weiten Bereich von räumlichen und zeitlichen Skalen. Die Lösung der thermischen Windgleichung entlang der mit den Isobaren identischen charakteristischen Kurven liefert atmosphärische Gleichgewichtszustände mit durch vorgegebenem Grundstrom einstellbarer Neigung zu(barotropen und baroklinen)Instabilitäten, die für die Entwicklung von Zyklonen wesentlich sind. Im Gegensatz zu früheren Arbeiten sind diese Zustände direkt im z-System(Höhe in Metern)definiert und müssen nicht aus Druckkoordinaten übertragen werden.Mit diesen Zuständen, sowohl als Referenzzustand, von dem lediglich die Abweichungen numerisch betrachtet werden, und insbesondere auch als Startzustand, der einer kleinen Störung unterliegt, werden verschiedene Studien der Simulation von barotroper und barokliner Instabilität durchgeführt. Hervorzuheben ist dabei die durch die Formulierung von Grundströmen mit einstellbarer Baroklinität ermöglichte simulationsgestützte Studie des Grades der baroklinen Instabilität verschiedener Wellenlängen in Abhängigkeit von statischer Stabilität und vertikalem Windgradient als Entsprechung zu Stabilitätskarten aus theoretischen Betrachtungen in der Literatur. N2 - The dynamics of the Earth’s atmosphere encompass a range from microphysical turbulence over convective processes and cloud formation up to planetary wave patterns. For weather forecasting and the investigation of climate over decades and centuries, these are subject to modelling with numerical methods. With progressing development of computer technology, re-development of the dynamical cores of climate models is in order to properly handle processes covered by the increasing resolution. The dynamical core of a model consists of the adaptation(discretization)of the basic equations for the dynamics of mass, energy and momentum for solving them numerically employing computers. The presented work investigates the applicability of a low-order Discontinuous Galerkin (DG) method for atmospheric applications. With equations that include external forces like gravitation and the Coriolis force, that is not given by theory. Necessary changes for stabilizing the method without resorting to slope limiters are presented. For the unmodified method, the basic inability to properly keep atmospheric balances is demonstrated. The developed stabilized model reproduces a set of standard test cases in a wide range of spatial and temporal scales. The solution of the termal wind equation along its characteristics curves, those being identical to the isobars, produces balanced atmospheric states with tunable (barotropic and baroclinic) instability via a prescribed zonal wind field. The constructed instability directly relates to the generation of cyclones. In contrast to earlier works, these balanced states are directly given in the z system (height in meters), without need for elaborate conversion from pressure coordinates. With these constructed states, both as reference state, the deviations from which being considered numerically, and as especially as initial condition subject to a small perturbation, several studies of barotropic and baroclinic instability are conducted via simulations. Particularily, the construction of steady states with configurable zonal flows of certain baroclinity facilitates a simulation-based study of baroclinic instability of differing wavelengths, depending on static stability and vertical wind gradient, in correspondence with stability maps from theoretical considerations in the literature. KW - Atmosphärenmodellierung KW - Unstetiges Galerkin-Verfahren KW - Multiskale KW - Barokline Instabilität KW - thermische Windgleichung KW - atmospheric modelling KW - discontinuous Galerkin method KW - multi-scale KW - baroclinic instability KW - thermal wind equation Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-70687 ER - TY - JOUR A1 - Klein, Eckart T1 - Entstehung des Grundgesetzes der Bundesrepublik Deutschland JF - Verfassungsentwicklung in Russland und Deutschland : Materialien des russisch-deutschen Symposiums anlässlich des 20. Jahrestages der Verfassung der Russischen Föderation am 25. und 26. September 2013 an der Universität Potsdam Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-70856 SP - 9 EP - 16 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Franzke, Jochen A1 - Büchner, Christiane T1 - Entstehung der Verfassung des Landes Brandenburg JF - Verfassungsentwicklung in Russland und Deutschland : Materialien des russisch-deutschen Symposiums anlässlich des 20. Jahrestages der Verfassung der Russischen Föderation am 25. und 26. September 2013 an der Universität Potsdam Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-70868 SP - 17 EP - 27 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Doroshenko, Egor N. T1 - Änderungen der Verfassung der Russischen Föderation JF - Verfassungsentwicklung in Russland und Deutschland : Materialien des russisch-deutschen Symposiums anlässlich des 20. Jahrestages der Verfassung der Russischen Föderation am 25. und 26. September 2013 an der Universität Potsdam Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-70872 SP - 29 EP - 36 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Thiele, Carmen T1 - Stabilität und Dynamik der Verfassungsprinzipien des Grundgesetzes der Bundesrepublik Deutschland JF - Verfassungsentwicklung in Russland und Deutschland : Materialien des russisch-deutschen Symposiums anlässlich des 20. Jahrestages der Verfassung der Russischen Föderation am 25. und 26. September 2013 an der Universität Potsdam Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-70908 SP - 55 EP - 64 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - THES A1 - Herenz, Peter T1 - A study of the absorption characteristics of gaseous galaxy halos in the local Universe T1 - Untersuchung von Absorptionsstrukturen in den gasförmigen Halos von Galaxien im lokalen Universum. N2 - Today, it is well known that galaxies like the Milky Way consist not only of stars but also of gas and dust. The galactic halo, a sphere of gas that surrounds the stellar disk of a galaxy, is especially interesting. It provides a wealth of information about in and outflowing gaseous material towards and away from galaxies and their hierarchical evolution. For the Milky Way, the so-called high-velocity clouds (HVCs), fast moving neutral gas complexes in the halo that can be traced by absorption-line measurements, are believed to play a crucial role in the overall matter cycle in our Galaxy. Over the last decades, the properties of these halo structures and their connection to the local circumgalactic and intergalactic medium (CGM and IGM, respectively) have been investigated in great detail by many different groups. So far it remains unclear, however, to what extent the results of these studies can be transferred to other galaxies in the local Universe. In this thesis, we study the absorption properties of Galactic HVCs and compare the HVC absorption characteristics with those of intervening QSO absorption-line systems at low redshift. The goal of this project is to improve our understanding of the spatial extent and physical conditions of gaseous galaxy halos in the local Universe. In the first part of the thesis we use HST /STIS ultraviolet spectra of more than 40 extragalactic background sources to statistically analyze the absorption properties of the HVCs in the Galactic halo. We determine fundamental absorption line parameters including covering fractions of different weakly/intermediately/highly ionized metals with a particular focus on SiII and MgII. Due to the similarity in the ionization properties of SiII and MgII, we are able to estimate the contribution of HVC-like halo structures to the cross section of intervening strong MgII absorbers at z = 0. Our study implies that only the most massive HVCs would be regarded as strong MgII absorbers, if the Milky Way halo would be seen as a QSO absorption line system from an exterior vantage point. Combining the observed absorption-cross section of Galactic HVCs with the well-known number density of intervening strong MgII absorbers at z = 0, we conclude that the contribution of infalling gas clouds (i.e., HVC analogs) in the halos of Milky Way-type galaxies to the cross section of strong MgII absorbers is 34%. This result indicates that only about one third of the strong MgII absorption can be associated with HVC analogs around other galaxies, while the majority of the strong MgII systems possibly is related to galaxy outflows and winds. The second part of this thesis focuses on the properties of intervening metal absorbers at low redshift. The analysis of the frequency and physical conditions of intervening metal systems in QSO spectra and their relation to nearby galaxies offers new insights into the typical conditions of gaseous galaxy halos. One major aspect in our study was to regard intervening metal systems as possible HVC analogs. We perform a detailed analysis of absorption line properties and line statistics for 57 metal absorbers along 78 QSO sightlines using newly-obtained ultraviolet spectra obtained with HST /COS. We find clear evidence for bimodal distribution in the HI column density in the absorbers, a trend that we interpret as sign for two different classes of absorption systems (with HVC analogs at the high-column density end). With the help of the strong transitions of SiII λ1260, SiIII λ1206, and CIII λ977 we have set up Cloudy photoionization models to estimate the local ionization conditions, gas densities, and metallicities. We find that the intervening absorption systems studied by us have, on average, similar physical conditions as Galactic HVC absorbers, providing evidence that many of them represent HVC analogs in the vicinity of other galaxies. We therefore determine typical halo sizes for SiII, SiIII, and CIII for L = 0.01L∗ and L = 0.05L∗ galaxies. Based on the covering fractions of the different ions in the Galactic halo, we find that, for example, the typical halo size for SiIII is ∼ 160 kpc for L = 0.05L∗ galaxies. We test the plausibility of this result by searching for known galaxies close to the QSO sightlines and at similar redshifts as the absorbers. We find that more than 34% of the measured SiIII absorbers have galaxies associated with them, with the majority of the absorbers indeed being at impact parameters ρ ≤160 kpc. N2 - Galaxien bestehen nicht nur aus Planeten und Sternen, sondern sind u.a. auch von einer Hülle aus Gas und Staub, dem Halo, umgeben. Dieser Halo spielt für die Entwicklung der Galaxie eine zentrale Rolle, da er mit der galaktischen Scheibe wechselwirken kann. Für das Verständnis des galaktischen Materiekreislaufs ist es daher entscheidend, die Prozesse und Vorgänge sowie das Zusammenspiel der verschiedenen Gasphasen in diesem Übergangsbereich zum intergalaktischen Medium charakterisieren und verstehen zu können. In der vorliegenden Arbeit werden lokale Phänomene, die sogenannten Hochgeschwindigkeitswolken (HVCs), im Halo der Milchstraße mit Hilfe des Hubble-Weltraumteleskops analysiert und ausgewertet. Im Gegensatz zu dem normalen Halo Gas bewegen sich diese HVCs mit ungewöhnlich hohen Geschwindigkeiten durch die ̈ äußeren Bereiche der Milchstraße. Sie passen daher nicht in das Galaktische Ge- schwindigkeitsmodell und stellen eine eigene, wichtige Klasse von Objekten dar, welche mit der Galaxie wechselwirken und diese beeinflussen. Für die Analyse dieser HVCs werden mehr als 40 Spektren von extragalaktischen Hintergrundquellen statistisch untersucht, um u.a. den Bedeckungsanteil von verschiedenen niedrig-/mittel- und hochionisierten Metallen zu ermitteln. Wegen der Ähnlichkeit der Ionisationsparameter von einfach ionisiertem Silizium, SiII, und einfach ionisiertem Magnesium, MgII, ist es möglich, den Beitrag von HVCs zum Wirkungsquerschnitt von starken MgII Absorbern im lokalen Universum zu bestimmen. Es stellt sich heraus, dass, würde man von außen auf die Milchstraße schauen, Galaktische HVCs etwa 52 % zum totalen Wirkungsquerschnitt von starken MgII Absorptionssystemen in der Milchstraße beitragen. Weiterhin ergibt sich, dass nur etwa ein Drittel der starken MgII Absorptionssysteme in der Umgebung von Milchstraßen-ähnlichen Galaxien als HVC Gegenstücke identifziert werden kann. Betrachtet man die große Anzahl an bekannten MgII Absorptionssystemen folgt daraus, dass das HVC-Phänomen nicht alleine auf unsere Galaxie beschränkt ist, sondern im Gegenteil, weit verbreitet zu sein scheint. Weiterhin werden die Eigenschaften von Metallsystemen bei niedriger Rotverschiebung in Quasarspektren analysiert. Die Suche nach extragalaktischen Metallsystemen in einer Vielzahl von Spektren und deren statistische Auswertung bezogen auf ihre Ursprungsgalaxien ermöglicht es, neue Erkenntnisse über die typische Struktur von Halos Milchstraßen-ähnlicher Galaxien zu erlangen. Eine der Hauptfragestellungen ist die Identifizierung von entfernten Metallsystemen als HVC-Analoga. Dazu wurden weitere Quasarspektren des Hubble-Teleskops ausgewertet und mit den Ergebnissen über Galaktische HVCs verglichen. Es zeigt sich hierbei, dass z.B. in der Säulendichteverteilung von neutralem Wasserstoff eine deutliche zweikomponentige Struktur zu erkennen ist. Diese könnte das Resultat von zwei verschiedenen Absorber Populationen sein, wobei eine HVC-ähnliche Eigenschaften aufweist. Diese Absorptionssysteme besitzen im Mittel sehr ähnliche Eigenschaften wie Galaktische Absorber, z.B. in Bezug auf die Eigenschaften des Gases oder dessen Zusammensetzung. Das impliziert u.a., dass sich auch dazugehörige Galaxien innerhalb eines bestimmten Abstandes um diese Absorber befinden sollten. Diese Vermutung wird anhand der Daten exemplarisch für zweichfach ionisiertes Silizium, SiII, untersucht. Es stellt sich heraus, dass sich in mehr als 34 % der Fälle zugehörige Galaxien bei SiIII Absorbern befinden, wobei die Mehrheit sogar innerhalb des von uns ermittelten mittleren Detektionsradius von 160 kpc zu finden ist. Allgemein können wir viele Hinweise darauf finden, dass das HVC-Phänomen nicht nur auf die Milchstraße beschränkt, sondern weit verbreitet ist. Zusätzlich scheinen Metallsysteme in Quasarspektren gute Indikatoren für HVC-Analoga in der Umgebung von anderen entfernten Galaxien zu sein. KW - Halo KW - Hochgeschwindigkeitswolken KW - Galaxien KW - Spektroskopie KW - halo KW - high-velocity-clouds KW - galaxies KW - spectroscopy Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-70513 ER - TY - THES A1 - Raiser, Christoph T1 - Kompromisse im Europäischen Parlament : eine kultursoziologische Analyse von Entscheidungsprozessen in einer supranationalen Institution T1 - Compromise in the European Parliament : a cultural sociological approach to decision-making processes in a supranational institution N2 - Das Europäische Parlament ist zweifelsohne die mächtigste parlamentarische Versammlung auf supranationaler Ebene. Das provoziert die Frage, wie Entscheidungen in diesem Parlament gefällt werden und wie sie begründet werden können. Darin liegt das Hauptanliegen dieser Arbeit, die zur Beantwortung dieser Frage auf soziologische Ansätze der Erklärung sozialen Handelns zurückgreift und damit einen neuen Zugang zur Beobachtung parlamentarischen Handelns schafft. Dabei arbeitet sie heraus, wie wichtig es ist, bei der Analyse politischer Entscheidungsprozesse zu beachten, wie politische Probleme von Akteuren interpretiert und gegenüber Verhandlungspartnern dargestellt werden. An den Fallbeispielen der Entscheidungsprozesse zur Dienstleistungsrichtlinie, zur Chemikalien-Verordnung REACH und dem TDIP (CIA)-Ausschuss in der Legislaturperiode 2004–2009, wird der soziale Mechanismus dargestellt, der hinter Einigungen im Europäischen Parlament steckt. Kultur als Interpretation der Welt wird so zum Schlüssel des Verständnisses politischer Entscheidungen auf supranationaler Ebene. N2 - The European Parliament is the most powerful parliamentary assembly on the supranational level. However, the question of how and why decisions are being taken in this parliament has been insufficiently addressed so far. This is the main aim of this book, which draws on sociological theories for explaining social action and thus opens up a new approach to the analysis of parliamentary action. It argues that it is necessary to take into account how actors interpret political problems and how they relate to their counterparts in negotiations. In three case studies on decision-making processes in the 6th European Parliament between 2004 and 2009 - Services Directive, REACH and the TDIP (CIA-)committee – the study reconstructs the social mechanism behind compromise in the EP. Culture as the way actors attach meaning to the world is the key to understanding political decisions on the supranational level. KW - Europäisches Parlament KW - Kompromiss KW - Kultur KW - Narration KW - Netzwerkanalyse KW - European Parliament KW - Compromise KW - Culture KW - Narration KW - Network analysis Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-69560 SN - 978-3-86956-283-4 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - THES A1 - Rogge-Solti, Andreas T1 - Probabilistic Estimation of Unobserved Process Events T1 - Probabilistische Abschätzung Unbeobachteter Prozessereignisse N2 - Organizations try to gain competitive advantages, and to increase customer satisfaction. To ensure the quality and efficiency of their business processes, they perform business process management. An important part of process management that happens on the daily operational level is process controlling. A prerequisite of controlling is process monitoring, i.e., keeping track of the performed activities in running process instances. Only by process monitoring can business analysts detect delays and react to deviations from the expected or guaranteed performance of a process instance. To enable monitoring, process events need to be collected from the process environment. When a business process is orchestrated by a process execution engine, monitoring is available for all orchestrated process activities. Many business processes, however, do not lend themselves to automatic orchestration, e.g., because of required freedom of action. This situation is often encountered in hospitals, where most business processes are manually enacted. Hence, in practice it is often inefficient or infeasible to document and monitor every process activity. Additionally, manual process execution and documentation is prone to errors, e.g., documentation of activities can be forgotten. Thus, organizations face the challenge of process events that occur, but are not observed by the monitoring environment. These unobserved process events can serve as basis for operational process decisions, even without exact knowledge of when they happened or when they will happen. An exemplary decision is whether to invest more resources to manage timely completion of a case, anticipating that the process end event will occur too late. This thesis offers means to reason about unobserved process events in a probabilistic way. We address decisive questions of process managers (e.g., "when will the case be finished?", or "when did we perform the activity that we forgot to document?") in this thesis. As main contribution, we introduce an advanced probabilistic model to business process management that is based on a stochastic variant of Petri nets. We present a holistic approach to use the model effectively along the business process lifecycle. Therefore, we provide techniques to discover such models from historical observations, to predict the termination time of processes, and to ensure quality by missing data management. We propose mechanisms to optimize configuration for monitoring and prediction, i.e., to offer guidance in selecting important activities to monitor. An implementation is provided as a proof of concept. For evaluation, we compare the accuracy of the approach with that of state-of-the-art approaches using real process data of a hospital. Additionally, we show its more general applicability in other domains by applying the approach on process data from logistics and finance. N2 - Unternehmen versuchen Wettbewerbsvorteile zu gewinnen und die Kundenzufriedenheit zu erhöhen. Um die Qualität und die Effizienz ihrer Prozesse zu gewährleisten, wenden Unternehmen Geschäftsprozessmanagement an. Hierbei spielt die Prozesskontrolle im täglichen Betrieb eine wichtige Rolle. Prozesskontrolle wird durch Prozessmonitoring ermöglicht, d.h. durch die Überwachung des Prozessfortschritts laufender Prozessinstanzen. So können Verzögerungen entdeckt und es kann entsprechend reagiert werden, um Prozesse wie erwartet und termingerecht beenden zu können. Um Prozessmonitoring zu ermöglichen, müssen prozessrelevante Ereignisse aus der Prozessumgebung gesammelt und ausgewertet werden. Sofern eine Prozessausführungsengine die Orchestrierung von Geschäftsprozessen übernimmt, kann jede Prozessaktivität überwacht werden. Aber viele Geschäftsprozesse eignen sich nicht für automatisierte Orchestrierung, da sie z.B. besonders viel Handlungsfreiheit erfordern. Dies ist in Krankenhäusern der Fall, in denen Geschäftsprozesse oft manuell durchgeführt werden. Daher ist es meist umständlich oder unmöglich, jeden Prozessfortschritt zu erfassen. Zudem ist händische Prozessausführung und -dokumentation fehleranfällig, so wird z.B. manchmal vergessen zu dokumentieren. Eine Herausforderung für Unternehmen ist, dass manche Prozessereignisse nicht im Prozessmonitoring erfasst werden. Solch unbeobachtete Prozessereignisse können jedoch als Entscheidungsgrundlage dienen, selbst wenn kein exaktes Wissen über den Zeitpunkt ihres Auftretens vorliegt. Zum Beispiel ist bei der Prozesskontrolle zu entscheiden, ob zusätzliche Ressourcen eingesetzt werden sollen, wenn eine Verspätung angenommen wird. Diese Arbeit stellt einen probabilistischen Ansatz für den Umgang mit unbeobachteten Prozessereignissen vor. Dabei werden entscheidende Fragen von Prozessmanagern beantwortet (z.B. "Wann werden wir den Fall beenden?", oder "Wann wurde die Aktivität ausgeführt, die nicht dokumentiert wurde?"). Der Hauptbeitrag der Arbeit ist die Einführung eines erweiterten probabilistischen Modells ins Geschäftsprozessmanagement, das auf stochastischen Petri Netzen basiert. Dabei wird ein ganzheitlicher Ansatz zur Unterstützung der einzelnen Phasen des Geschäftsprozesslebenszyklus verfolgt. Es werden Techniken zum Lernen des probabilistischen Modells, zum Vorhersagen des Zeitpunkts des Prozessendes, zum Qualitätsmanagement von Dokumentationen durch Erkennung fehlender Einträge, und zur Optimierung von Monitoringkonfigurationen bereitgestellt. Letztere dient zur Auswahl von relevanten Stellen im Prozess, die beobachtet werden sollten. Diese Techniken wurden in einer quelloffenen prototypischen Anwendung implementiert. Zur Evaluierung wird der Ansatz mit existierenden Alternativen an echten Prozessdaten eines Krankenhauses gemessen. Die generelle Anwendbarkeit in weiteren Domänen wird examplarisch an Prozessdaten aus der Logistik und dem Finanzwesen gezeigt. KW - Geschäftsprozessmanagement KW - stochastische Petri Netze KW - Bayessche Netze KW - Probabilistische Modelle KW - Vorhersage KW - Fehlende Daten KW - Process Mining KW - business process management KW - stochastic Petri nets KW - Bayesian networks KW - probabilistic models KW - prediction KW - missing data KW - process mining Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-70426 ER - TY - THES A1 - Ahmad, Nadeem T1 - People centered HMI’s for deaf and functionally illiterate users T1 - Menschen zentrierte Mensch-Maschine-Schnittstellen für Schwerhörige und ungeschulte Anwender N2 - The objective and motivation behind this research is to provide applications with easy-to-use interfaces to communities of deaf and functionally illiterate users, which enables them to work without any human assistance. Although recent years have witnessed technological advancements, the availability of technology does not ensure accessibility to information and communication technologies (ICT). Extensive use of text from menus to document contents means that deaf or functionally illiterate can not access services implemented on most computer software. Consequently, most existing computer applications pose an accessibility barrier to those who are unable to read fluently. Online technologies intended for such groups should be developed in continuous partnership with primary users and include a thorough investigation into their limitations, requirements and usability barriers. In this research, I investigated existing tools in voice, web and other multimedia technologies to identify learning gaps and explored ways to enhance the information literacy for deaf and functionally illiterate users. I worked on the development of user-centered interfaces to increase the capabilities of deaf and low literacy users by enhancing lexical resources and by evaluating several multimedia interfaces for them. The interface of the platform-independent Italian Sign Language (LIS) Dictionary has been developed to enhance the lexical resources for deaf users. The Sign Language Dictionary accepts Italian lemmas as input and provides their representation in the Italian Sign Language as output. The Sign Language dictionary has 3082 signs as set of Avatar animations in which each sign is linked to a corresponding Italian lemma. I integrated the LIS lexical resources with MultiWordNet (MWN) database to form the first LIS MultiWordNet(LMWN). LMWN contains information about lexical relations between words, semantic relations between lexical concepts (synsets), correspondences between Italian and sign language lexical concepts and semantic fields (domains). The approach enhances the deaf users’ understanding of written Italian language and shows that a relatively small set of lexicon can cover a significant portion of MWN. Integration of LIS signs with MWN made it useful tool for computational linguistics and natural language processing. The rule-based translation process from written Italian text to LIS has been transformed into service-oriented system. The translation process is composed of various modules including parser, semantic interpreter, generator, and spatial allocation planner. This translation procedure has been implemented in the Java Application Building Center (jABC), which is a framework for extreme model driven design (XMDD). The XMDD approach focuses on bringing software development closer to conceptual design, so that the functionality of a software solution could be understood by someone who is unfamiliar with programming concepts. The transformation addresses the heterogeneity challenge and enhances the re-usability of the system. For enhancing the e-participation of functionally illiterate users, two detailed studies were conducted in the Republic of Rwanda. In the first study, the traditional (textual) interface was compared with the virtual character-based interactive interface. The study helped to identify usability barriers and users evaluated these interfaces according to three fundamental areas of usability, i.e. effectiveness, efficiency and satisfaction. In another study, we developed four different interfaces to analyze the usability and effects of online assistance (consistent help) for functionally illiterate users and compared different help modes including textual, vocal and virtual character on the performance of semi-literate users. In our newly designed interfaces the instructions were automatically translated in Swahili language. All the interfaces were evaluated on the basis of task accomplishment, time consumption, System Usability Scale (SUS) rating and number of times the help was acquired. The results show that the performance of semi-literate users improved significantly when using the online assistance. The dissertation thus introduces a new development approach in which virtual characters are used as additional support for barely literate or naturally challenged users. Such components enhanced the application utility by offering a variety of services like translating contents in local language, providing additional vocal information, and performing automatic translation from text to sign language. Obviously, there is no such thing as one design solution that fits for all in the underlying domain. Context sensitivity, literacy and mental abilities are key factors on which I concentrated and the results emphasize that computer interfaces must be based on a thoughtful definition of target groups, purposes and objectives. N2 - Das Ziel und die Motivation hinter dieser Forschungsarbeit ist es, Anwendungen mit benutzerfreundlichen Schnittstellen für Gehörlose und Analphabeten bereitzustellen, welche es ihnen ermöglichen ohne jede menschliche Unterstützung zu arbeiten. Obwohl es in den letzten Jahren technologische Fortschritte gab, garantiert allein die Verfügbarkeit von Technik nicht automatisch die Zugänglichkeit zu Informations- und Kommunikationstechnologien (IKT). Umfangreiche Verwendung von Texten in Menüs bis hin zu Dokumenten führen dazu, dass Gehörlose und funktionale Analphabeten auf diese Dienste, die auf Computersystemen existieren, nicht zugreifen können. Folglich stellen die meisten bestehenden EDV-Anwendungen für diejenigen eine Hürde dar, die nicht fließend lesen können. Onlinetechnologien für solche Art von Gruppen sollten in kontinuierlichem Austausch mit den Hauptnutzern entwickelt werden und gründliche Untersuchung ihrer Einschränkungen, Anforderungen und Nutzungsbarrieren beinhalten. In dieser Forschungsarbeit untersuche ich vorhandene Tools im Bereich von Sprach-, Web- und anderen Multimedia-Technologien, um Lernlücken zu identifizieren und Wege zu erforschen, welche die Informationsalphabetisierung für Gehörlose und Analphabeten erweitert. Ich arbeitete an der Entwicklung einer nutzerzentrierten Schnittstelle, um die Fähigkeiten von Gehörlosen und Nutzern mit schlechter Alphabetisierung durch Verbesserung der lexikalischen Ressourcen und durch Auswertung mehrerer Multimediaschnittstellen zu erhöhen. Die Schnittstelle des plattformunabhängigen, italienischen Gebärdensprachen (LIS) Wörterbuches wurde entwickelt, um die lexikalischen Quellen für gehörlose Anwender zu verbessern. Ich integriere die LIS-lexikalischen Ressourcen mit der MultiWordNet (MWN)-Datenbank, um das erste LIS MultiWordNet (LMWN) zu bilden. LMWN enthält Informationen über lexikalische Beziehungen zwischen Wörtern, semantische Beziehungen zwischen lexikalischen Konzepten (Synsets), Gemeinsamkeiten zwischen italienischer und Gebärdensprache-lexikalischen Konzepten und semantischen Feldern (Domänen). Der Ansatz erhöht das Verständnis der tauben Nutzer geschriebene, italienischen Sprache zu verstehen und zeigt, dass ein relativ kleiner Satz an Lexika den wesentlichen Teil der MWN abdecken kann. Die Integration von LIS-Zeichen mit MWN machte es zu einem nützlichen Werkzeug für Linguistik und Sprachverarbeitung. Der regelbasierte Übersetzungsprozess von geschriebenem, italienischem Text zu LIS wurde in ein Service-orientiertes System überführt. Der Übersetzungsprozess besteht aus verschiedenen Modulen, einschließlich einem Parser, einem semantischen Interpreter, einem Generator und einem räumlichen Zuordnungs-Planer. Diese Übersetzungsprozedur wurde im Java Application Building Center (jABC) implementiert, welches ein Framework für das eXtrem Model Driven Design (XMDD) ist. Der XMDD-Ansatz konzentriert sich darauf die Softwareentwicklung näher zum konzeptionellen Design zu bringen, sodass die Funktionalität einer Softwarelösung von jemandem verstanden werden kann, der mit Programmierkonzepten unvertraut ist. Die Transformation richtet sich an die heterogene Herausforderung und verbessert die Wiederverwendbarkeit des Systems. Zur Verbesserung der E-Partizipation der funktionalen Analphabeten wurden zwei detaillierte Studien in der Republik Ruanda durchgeführt. In der ersten Studie wurde die traditionelle, textuelle Schnittstelle mit der virtuellen, charakterbasierten, interaktiven Schnittstelle verglichen. Diese Studie hat dazu beigetragen Barrieren der Benutzerfreundlichkeit zu identifizieren. Anwender evaluierten die Schnittstellen bezüglich drei grundlegender Bereiche der Benutzerfreundlichkeit: Effektivität, Effizienz und Zufriedenstellung bei der Problemlösung. In einer anderen Studie entwickelten wir vier verschiedene Schnittstellen, um die Benutzerfreundlichkeit und die Effekte der Online-Hilfe (konsequente Hilfe) für funktionale Analphabeten zu analysieren und verglichen verschiedene Hilfsmodi (einschließlich zusätzlicher Textinformationen, Audio-Unterstützung und mit Hilfe eines virtuellen Charakters) bezüglich der Verbesserung der Leistungsfähigkeit von teilweisen Analphabeten, um ihre Zielstellung zu erreichen. In unseren neu gestalteten Schnittstellen wurden Anweisungen automatisch in die Swahili Sprache übersetzt. Alle Schnittstellen wurden auf der Grundlage der Aufgabenbewältigung, des Zeitaufwands, der System Usability Scale (SUS) Rate und der Anzahl der Hilfegesuche bewertet. Die Ergebnisse zeigen, dass die Leistungsfähigkeit der teilweisen Analphabeten bei Nutzung der Online-Hilfe signifikant verbessert wurde. Diese Dissertation stellt somit einen neuen Entwicklungsansatz dar, bei welchem virtuelle Charaktere als zusätzliche Unterstützung genutzt werden, um Nutzer, die kaum lesen können oder sonstig eingeschränkt sind, zu unterstützen. Solche Komponenten erweitern die Anwendungsnutzbarkeit indem sie eine Vielzahl von Diensten wie das Übersetzen von Inhalten in eine Landessprache, das Bereitstellen von zusätzlichen akustisch, gesprochenen Informationen und die Durchführung einer automatischen Übersetzung von Text in Gebärdensprache bereitstellen. Offensichtlich gibt ist keine One Design-Lösung, die für alle zugrundeliegenden Domänen passt. Kontextsensitivität, Alphabetisierung und geistigen Fähigkeiten sind Schlüsselfaktoren, auf welche ich mich konzentriere. Die Ergebnisse unterstreichen, dass Computerschnittstellen auf einer exakten Definition der Zielgruppen, dem Zweck und den Zielen basieren müssen. KW - Mensch-Computer-Interaktion KW - assistive Technologien KW - HCI KW - user interfaces KW - sign language KW - online assistance KW - assistive technologies Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-70391 ER - TY - THES A1 - Tenenboim, Yehezkel T1 - Characterization of a Chlamydomonas protein involved in cell division and autophagy T1 - Charakterisierung eines an Zellteilung und Autophagie beteiligten Chlamydomonas-Proteins N2 - The contractile vacuole (CV) is an osmoregulatory organelle found exclusively in algae and protists. In addition to expelling excessive water out of the cell, it also expels ions and other metabolites and thereby contributes to the cell's metabolic homeostasis. The interest in the CV reaches beyond its immediate cellular roles. The CV's function is tightly related to basic cellular processes such as membrane dynamics and vesicle budding and fusion; several physiological processes in animals, such as synaptic neurotransmission and blood filtration in the kidney, are related to the CV's function; and several pathogens, such as the causative agents of sleeping sickness, possess CVs, which may serve as pharmacological targets. The green alga Chlamydomonas reinhardtii has two CVs. They are the smallest known CVs in nature, and they remain relatively untouched in the CV-related literature. Many genes that have been shown to be related to the CV in other organisms have close homologues in C. reinhardtii. We attempted to silence some of these genes and observe the effect on the CV. One of our genes, VMP1, caused striking, severe phenotypes when silenced. Cells exhibited defective cytokinesis and aberrant morphologies. The CV, incidentally, remained unscathed. In addition, mutant cells showed some evidence of disrupted autophagy. Several important regulators of the cell cycle as well as autophagy were found to be underexpressed in the mutant. Lipidomic analysis revealed many meaningful changes between wild-type and mutant cells, reinforcing the compromised-autophagy observation. VMP1 is a singular protein, with homologues in numerous eukaryotic organisms (aside from fungi), but usually with no relatives in each particular genome. Since its first characterization in 2002 it has been associated with several cellular processes and functions, namely autophagy, programmed cell-death, secretion, cell adhesion, and organelle biogenesis. It has been implicated in several human diseases: pancreatitis, diabetes, and several types of cancer. Our results reiterate some of the observations in VMP1's six reported homologues, but, importantly, show for the first time an involvement of this protein in cell division. The mechanisms underlying this involvement in Chlamydomonas, as well as other key aspects, such as VMP1's subcellular localization and interaction partners, still await elucidation. N2 - Die kontraktile Vakuole ist ein osmoregulatorisches Organell, das ausschließlich in Algen und Protisten vorkommt. Zusätzlich zu ihrer Rolle als Ausstoßer überflüßigen Wassers aus der Zelle heraus, stößt sie auch Ionen und andere Metaboliten aus, und trägt dabei zur metabolischen Homöostase der Zelle bei. Das Interesse an der kontraktilen Vakuole erstreckt sich über seine unmittelbare zelluläre Rolle hinaus. Die Funktion der kontraktilen Vakuole ist mit einigen grundsätzlichen zellulären Verfahren, wie Membrandynamik und Vesikelknospung und -fusion, verwandt; einige physiologische Verfahren in Tieren, zum Beispiel synaptische Neurotransmission und das Filtrieren des Blutes in den Nieren, sind mit der Funktion der Vakuole eng verwandt; und einige Pathogene—der Ursacher der Schlafkrankheit als Beispiel—besitzen kontraktile Vakuolen, die als Ziele von Medikamenten dienen könnten. Die grüne Alge Chlamydomonas reinhardtii verfügt über zwei Vakuolen. Sie sind die kleinsten bekannten in der Natur, und bleiben bisher verhältnismäßig unerforscht. Viele Gene, die in anderen Organismen als kontraktile-Vakuole-bezogen erwiesen wurden, haben Homologe in C. reinhardtii. Wir versuchten, diese Gene auszuschalten und den Einfluss auf die Vakuole zu beobachten. Die Ausschaltung eines unserer Gene, VMP1, verursachte starke, beachtliche Phänotype. Die Zellen zeigten gestörte Zytokinese und aberrante Zellformen. Die kontraktile Vakuole blieb jedoch verschont. Des Weiteren zeigten Mutantzellen einige Hinweise auf gestörte Autophagie. Einige wichtige Gene des Zellzyklus und der Autophagie waren unterexprimiert in Mutantzellen. Lipidomische Analyse zeigte mehrere bedeutsame Unterschiede zwischen Wildtyp und Mutant, die die Beobachtungen der gestörten Autophagie verstärkten. VMP1 ist ein singularisches Protein, mit Homologen in zähligen eukaryotischen Organismen (jedoch nicht in Pilzen), aber üblicherweise ohne Verwandte in den jeweiligen Genomen. Seit seiner Erstcharakterisierung 2002 wurde es mit etlichen zellulären Verfahren, wie Autophagie, programmiertem Zelltod, Sekretion, Zelladhäsion, und Biogenese der Organellen, assoziiert. Es wurde auch mit einigen menschlichen Krankheiten wie Diabetes, Pankreatitis, und einigen Arten von Krebs in Verbindung gebracht. Unsere Ergebnisse wiederholen einige Beobachtungen in anderen Organismen, zeigen dennoch zum ersten Mal eine Beteiligung von VMP1 an der Zellteilung. Die unterliegenden Mechanismen dieser Beteiligung in Chlamydomonas, sowie andere wichtige Aspekte, etwa die subzelluläre Lokalisierung von VMP1 und dessen Interaktionspartner, warten noch auf Aufklärung. KW - VMP1 KW - autophagy KW - cytokinesis KW - chlamydomonas Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-70650 ER - TY - GEN A1 - Schott, Juliane A1 - Kretzschmar, Jerome A1 - Acker, Margret A1 - Eidner, Sascha A1 - Kumke, Michael Uwe A1 - Drobot, Björn A1 - Barkleit, Astrid A1 - Taut, Steffen A1 - Brendler, Vinzenz A1 - Stumpf, Thorsten T1 - Formation of a Eu(III) borate solid species from a weak Eu(III) borate complex in aqueous solution N2 - In the presence of polyborates (detected by 11B-NMR) the formation of a weak Eu(III) borate complex (lg β11 ∼ 2, estimated) was observed by time-resolved laser-induced fluorescence spectroscopy (TRLFS). This complex is a precursor for the formation of a solid Eu(III) borate species. The formation of this solid in solution was investigated by TRLFS as a function of the total boron concentration: the lower the total boron concentration, the slower is the solid formation. The solid Eu(III) borate was characterized by IR spectroscopy, powder XRD and solid-state TRLFS. The determination of the europium to boron ratio portends the existence of pentaborate units in the amorphous solid. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 277 Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-98774 ER - TY - GEN A1 - Plehn, Thomas A1 - Megow, Jörg A1 - May, Volkhard T1 - Concerted charge and energy transfer processes in a highly flexible fullerene–dye system BT - a mixed quantum–classical study N2 - Photoinduced excitation energy transfer and accompanying charge separation are elucidated for a supramolecular system of a single fullerene covalently linked to six pyropheophorbide-a dye molecules. Molecular dynamics simulations are performed to gain an atomistic picture of the architecture and the surrounding solvent. Excitation energy transfer among the dye molecules and electron transfer from the excited dyes to the fullerene are described by a mixed quantum–classical version of the Förster rate and the semiclassical Marcus rate, respectively. The mean characteristic time of energy redistribution lies in the range of 10 ps, while electron transfer proceeds within 150 ps. In between, on a 20 to 50 ps time-scale, conformational changes take place in the system. This temporal hierarchy of processes guarantees efficient charge separation, if the structure is exposed to a solvent. The fast energy transfer can adopt the dye excitation to the actual conformation. In this sense, the probability to achieve charge separation is large enough since any dominance of unfavorable conformations that exhibit a large dye–fullerene distance is circumvented. And the slow electron transfer may realize an averaging with respect to different conformations. To confirm the reliability of our computations, ensemble measurements on the charge separation dynamics are simulated and a very good agreement with the experimental data is obtained. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 279 Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-98791 ER - TY - GEN A1 - Węcławski, Marek K. A1 - Tasior, Mariusz A1 - Hammann, Tommy A1 - Cywiński, Piotr J. A1 - Gryko, Daniel T. T1 - From π-expanded coumarins to π-expanded pentacenes N2 - The synthesis of two novel types of π-expanded coumarins has been developed. Modified Knoevenagel bis-condensation afforded 3,9-dioxa-perylene-2,8-diones. Subsequent oxidative aromatic coupling or light driven electrocyclization reaction led to dibenzo-1,7-dioxacoronene-2,8-dione. Unparalleled synthetic simplicity, straightforward purification and superb optical properties have the potential to bring these perylene and coronene analogs towards various applications. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 280 Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-98822 ER - TY - GEN A1 - Ciuciu, Adina I. A1 - Cywiński, Piotr J. T1 - Two-photon polymerization of hydrogels – versatile solutions to fabricate well-defined 3D structures N2 - Hydrogels are cross-linked water-containing polymer networks that are formed by physical, ionic or covalent interactions. In recent years, they have attracted significant attention because of their unique physical properties, which make them promising materials for numerous applications in food and cosmetic processing, as well as in drug delivery and tissue engineering. Hydrogels are highly water-swellable materials, which can considerably increase in volume without losing cohesion, are biocompatible and possess excellent tissue-like physical properties, which can mimic in vivo conditions. When combined with highly precise manufacturing technologies, such as two-photon polymerization (2PP), well-defined three-dimensional structures can be obtained. These structures can become scaffolds for selective cell-entrapping, cell/drug delivery, sensing and prosthetic implants in regenerative medicine. 2PP has been distinguished from other rapid prototyping methods because it is a non-invasive and efficient approach for hydrogel cross-linking. This review discusses the 2PP-based fabrication of 3D hydrogel structures and their potential applications in biotechnology. A brief overview regarding the 2PP methodology and hydrogel properties relevant to biomedical applications is given together with a review of the most important recent achievements in the field. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 286 Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-99450 ER - TY - GEN A1 - Peitsch, Helmut T1 - Die Vorgeschichte der ‚Brecht-Lukács-Debatte‘ BT - Die ‚Spesen‘ zu Brechts ‚Sieg‘ T2 - Postprints der Universität Potsdam : Philosophische Reihe N2 - Werner Mittenzwei’s article of 1967, the title of which coined the term “Brecht-Lukács-Debatte”, is widely considered as a milestone in the development of East German literary criticism towards an “emancipation” from party politics. By placing Mittenzwei’s contribution in the wider context of discussions about the literature of the GDR, within the SED and the writers’ union as well as at international conferences, this article attempts to trace the emergence of “Umfunktionierung” both as a key term and in its official approval by the party. T3 - Zweitveröffentlichungen der Universität Potsdam : Philosophische Reihe - 113 Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-94165 SN - 1866-8380 IS - 113 ER - TY - GEN A1 - Berner, Elisabeth T1 - Albrecht Greule, Jörg Meier u. Arne Ziegler (Hgg.), Kanzleisprachenforschung. Ein internationales Handbuch / [rezensiert von] Elisabeth Berner N2 - Rezensiertes Werk: Albrecht Greule, Jörg Meier u. Arne Ziegler (Hgg.): Kanzleisprachenforschung. Ein internationales Handbuch. - Berlin u. Boston: de Gruyter 2012 - 680 S. - (XVI) T3 - Zweitveröffentlichungen der Universität Potsdam : Philosophische Reihe - 112 Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-94122 SP - 654 EP - 660 ER - TY - JOUR A1 - Ippolito, Antonella T1 - L' Inferno nel ciclo dei Contorni danteschi di Bonaventura Genelli JF - Dante e l' Arte N2 - Il ciclo dei trentasei Contorni danteschi del pittore e disegnatore berlinese Giovanni Bonaventura Genelli (1798-1868) rappresenta un interessante tentativo d’interpretare il testo della Commedia alla luce dei principi del classicismo tedesco, sull’esempio di Carstens e di Koch e, in parte di Flaxman da cui però Genelli si distingue per continua ricerca volta ad esprimere la plasticità delle forme. Caratteristica precipua delle sue illustrazioni è inoltre il ricorso continuo sia alla scultura antica che agli affreschi michelangioleschi della Sistina come repertorio iconografico. L’articolo approfondisce questi aspetti con particolare riferimento alle sedici tavole dedicate all’Inferno. N2 - The cycle of the 36 outline illustrations to Dante’s Divine Comedy represents a very interesting attempt towards a pictorial interpretation of the text inspired to the principles of the German classicism. Genelli is in this respect a follower of Carstens and Koch. The cleanliness of his line drawing with no shading and few background details is reminiscent of Flaxman; nonetheless, an evident mark of Genelli’s style is his permanent effort of exploring and expressing the dynamic plasticity of bodies. To represent Dante’s world he also draws on ancient sculpture as well as on Michelangelo’s Sistina frescoes as a iconographic repertoire. These aspects are here illustrated with special regard to the sixteen illustration of the Hell. KW - Inferno KW - illustrazioni KW - disegno KW - Neoclassicismo KW - arte tedesca Y1 - 2014 SN - 2385-5355 VL - 2014 IS - 1 SP - 241 EP - 270 PB - Univ. Autònoma de Barcelona CY - Bellaterra ER - TY - BOOK ED - Görtemaker, Manfred T1 - Otto Braun BT - ein preußischer Demokrat N2 - Otto Braun war von 1921 bis 1932 mit kurzen Unterbrechungen Ministerpräsident des Freistaates Preußen. Er war sowohl überzeugter sozialer Demokrat als auch Preuße und betrieb eine entschlossene Reformpolitik, die zwar umstritten war, sich aber stets im Rahmen der Legalität bewegte. Die Grenzen dieses Ansatzes wurden am Ende der Weimarer Republik deutlich, als seine Regierung mit dem sogenannten »Preußenschlag« vom 20. Juli 1932 entmachtet wurde und Braun in Verkennung der neuen machtpolitischen Realitäten vergeblich versuchte, mit legalen Maßnahmen dem illegalen Vorgehen der Reichsregierung entgegenzutreten. Y1 - 2014 SN - 978-3-89809-116-9 PB - Be.bra-Verl. CY - Berlin ER - TY - CHAP A1 - Görtemaker, Manfred T1 - Zwischen Demokratie und Diktatur BT - Otto Braun in der Weimarer Republik T2 - Otto Braun : ein preußischer Demokrat Y1 - 2014 SN - 978-3-89809-116-9 SP - 11 EP - 33 PB - Be.bra-Verl. CY - Berlin ER - TY - CHAP A1 - Apostolow, Markus A1 - Detjens, Florian T1 - Das wichtigste Postulat wahrer Demokratie BT - Otto Braun und die Freiheit der Presse T2 - Otto Braun : ein preußischer Demokrat Y1 - 2014 SN - 978-3-89809-116-9 SP - 35 EP - 57 PB - Be.bra-Verl. CY - Berlin ER - TY - GEN A1 - Cywiński, Piotr J. A1 - Nono, Katia Nchimi A1 - Charbonnière, Loïc J. A1 - Hammann, Tommy A1 - Löhmannsröben, Hans-Gerd T1 - Photophysical evaluation of a new functional terbium complex in FRET-based time-resolved homogenous fluoroassays N2 - A new functional luminescent lanthanide complex (LLC) has been synthesized with terbium as a central lanthanide ion and biotin as a functional moiety. Unlike in typical lanthanide complexes assembled via carboxylic moieties, in the presented complex, four phosphate groups are chelating the central lanthanide ion. This special chemical assembly enhances the complex stability in phosphate buffers conventionally used in biochemistry. The complex synthesis strategy and photophysical properties are described as well as the performance in time-resolved Förster Resonance Energy Transfer (FRET) assays. In those assays, this biotin-LLC transferred energy either to acceptor organic dyes (Cy5 or AF680) labelled on streptavidin or to quantum dots (QD655 or QD705) surface-functionalised with streptavidins. The permanent spatial donor–acceptor proximity is assured through strong and stable biotin–streptavidin binding. The energy transfer is evidenced from the quenching observed in donor emission and from a decrease in donor luminescence decay, both associated with simultaneous increase in acceptor intensity and in the decay time. The dye-based assays are realised in TRIS and in PBS, whereas QD-based systems are studied in borate buffer. The delayed emission analysis allows for quantifying the recognition process and for auto-fluorescence-free detection, which is particularly relevant for application in bioanalysis. In accordance with Förster theory, Förster-radii (R0) were found to be around 60 Å for organic dyes and around 105 Å for QDs. The FRET efficiency (η) reached 80% and 25% for dye and QD acceptors, respectively. Physical donor–acceptor distances (r) have been determined in the range 45–60 Å for organic dye acceptors, while for acceptor QDs between 120 Å and 145 Å. This newly synthesised biotin-LLC extends the class of highly sensitive analytical tools to be applied in the bioanalytical methods such as time-resolved fluoroimmunoassays (TR-FIA), luminescent imaging and biosensing. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 252 KW - acceptors KW - bioanalysis KW - contrast agents KW - europium KW - fluoroimmunoassay KW - labels KW - lanthanide luminescence KW - quantum dots KW - resonance energy-transfer Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-95390 SP - 6060 EP - 6067 ER - TY - THES A1 - Kipp, Anna Patricia T1 - Physiologische und Tumor-Assoziierte Funktionen von Selen und Selenoproteinen Y1 - 2014 ER - TY - THES A1 - Cao, Xianyong T1 - Vegetation and climate change in eastern continental Asia during the last 22 ka inferred from pollen data synthesis Y1 - 2014 ER - TY - BOOK ED - Lambrecht, Anna-Lena ED - Margaria, Tizian T1 - Process design for natural scientists BT - an agile model-driven approach T3 - Communications in computer and information science ; 500 N2 - This book presents an agile and model-driven approach to manage scientific workflows. The approach is based on the Extreme Model Driven Design (XMDD) paradigm and aims at simplifying and automating the complex data analysis processes carried out by scientists in their day-to-day work. Besides documenting the impact the workflow modeling might have on the work of natural scientists, this book serves three major purposes: 1. It acts as a primer for practitioners who are interested to learn how to think in terms of services and workflows when facing domain-specific scientific processes. 2. It provides interesting material for readers already familiar with this kind of tools, because it introduces systematically both the technologies used in each case study and the basic concepts behind them. 3. As the addressed thematic field becomes increasingly relevant for lectures in both computer science and experimental sciences, it also provides helpful material for teachers that plan similar courses. Y1 - 2014 SN - 978-3-662-45005-5 PB - Springer CY - Wiesbaden ER - TY - THES A1 - Giordano, Cristina T1 - A neglected world: transition metal nitride and metal carbide based nanostructures T1 - Eine vernachlässigte Welt: Übergangsmetallnitrid- und Metallcarbid-basierte Nanostrukturen BT - novel synthesis and future perspectives BT - neuartige Synthese und Zukunftsperspektiven N2 - Potentiality of nanosized materials has been largely proved but a closer look shows that a significant percentage of this research is related to oxides and metals, while the number drastically drops for metallic ceramics, namely transition metal nitrides and metal carbides. The lack of related publications do not reflect their potential but rather the difficulties related to their synthesis as dense and defect-free structures, fundamental prerequisites for advanced mechanical applications. The present habilitation work aims to close the gap between preparation and processing, indicating novel synthetic pathways for a simpler and sustainable synthesis of transition metal nitride (MN) and carbide (MC) based nanostructures and easier processing thereafter. In spite of simplicity and reliability, the designed synthetic processes allow the production of functional materials, with the demanded size and morphology. The goal was achieved exploiting classical and less-classical precursors, ranging from common metal salts and molecules (e.g. urea, gelatin, agar, etc), to more exotic materials, such as leafs, filter paper and even wood. It was found that the choice of precursors and reaction conditions makes it possible to control chemical composition (going for instance from metal oxides to metal oxy-nitrides to metal nitrides, or from metal nitrides to metal carbides, up to quaternary systems), size (from 5 to 50 nm) and morphology (going from mere spherical nanoparticles to rod-like shapes, fibers, layers, meso-porous and hierarchical structures, etc). The nature of the mixed precursors also allows the preparation of metal nitrides/carbides based nanocomposites, thus leading to multifunctional materials (e.g. MN/MC@C, MN/MC@PILs, etc) but also allowing dispersion in liquid media. Control over composition, size and morphology is obtained with simple adjustment of the main route, but also coupling it with processes such as electrospin, aerosol spray, bio-templating, etc. Last but not least, the nature of the precursor materials also allows easy processing, including printing, coating, casting, film and thin layers preparation, etc). The designed routes are, concept-wise, similar and they all start by building up a secondary metal ion-N/C precursor network, which converts, upon heat treatment, into an intermediate “glass”. This glass stabilizes the nascent nanoparticles during their nucleation and impairs their uncontrolled growth during the heat treatment (scheme 1). This way, one of the main problems related to the synthesis of MN/MC, i.e. the need of very high temperature, could also be overcome (from up to 2000°C, for classical synthesis, down to 700°C in the present cases). The designed synthetic pathways are also conceived to allow usage of non-toxic compounds and to minimize (or even avoid) post-synthesis purification, still bringing to phase pure and well-defined (crystalline) nanoparticles. This research aids to simplify the preparation of MN/MC, making these systems now readily available in suitable amounts both for fundamental and applied science. The prepared systems have been tested (in some cases for the first time) in many different fields, e.g. battery (MnN0.43@C shown a capacity stabilized at a value of 230 mAh/g, with coulombic efficiencies close to 100%), as alternative magnetic materials (Fe3C nanoparticles were prepared with different size and therefore different magnetic behavior, superparamagnetic or ferromagnetic, showing a saturation magnetization value up to 130 emu/g, i.e. similar to the value expected for the bulk material), as filters and for the degradation of organic dyes (outmatching the performance of carbon), as catalysts (both as active phase but also as active support, leading to high turnover rate and, more interesting, to tunable selectivity). Furthermore, with this route, it was possible to prepare for the first time, to the best of our knowledge, well-defined and crystalline MnN0.43, Fe3C and Zn1.7GeN1.8O nanoparticles via bottom-up approaches. Once the synthesis of these materials can be made straightforward, any further modification, combination, manipulation, is in principle possible and new systems can be purposely conceived (e.g. hybrids, nanocomposites, ferrofluids, etc). N2 - Materialien sind wichtige Hilfsmittel, die wir täglich benutzen, um unser Leben einfacher zu machen. Materialien werden durch ihre Eigenschaften charakterisiert. Manche sind stark, manche sind elektrisch, manche magnetisch, usw. Wenn ein Stoff auf die Dimension eines Virus verkleinert wird, zeigen sich besondere Veränderungen. War das Material groß z.B. elektrisch, hat es klein diese Eigenschaft nicht mehr. Groß war es glanzlos, aber klein ist es transparent... Das heißt, dass jedes bekannte Material prinzipiell ein neues Material werden kann, nur durch die Veränderung seiner Größe. Wegen dieser besonderen Entdeckung, ist seit vielen Jahren ein Großteil der Forschung Nanopartikeln gewidmet. Dennoch werden in diesem Gebiet meistens besondere Materialien untersucht, weil ihre Anwendungsfelder weithin bekannt und wichtig sind. Meine Forschung basiert auf der Untersuchung neuer oder weniger bekannter Materialien, nämlich Übergangsmetallnitride und Übergangsmetallcarbide als Nanostrukturen. Sie sind nicht so bekannt, weil sie nicht so einfach herzustellen waren (die klassische Synthese braucht eine hohe Temperatur bis 2000°C, und oft toxische Reaktionsmittel). Trotzdem sind sie sehr interessante Materialien, weil sie auf der Grenze zwischen reinen Metallen und reinen keramischen Materialien hinsichtlich der Eigenschaften liegen. Das heißt, sie sind z.B. sehr fest, aber auch leitend, sie haben einen hohen Schmelzpunkt und sind magnetisch, etc. Durch die hier vorgestellte Forschung wird die Synthese dieser Materialien einfacher gemacht, bei niedrigen Temperaturen, mit natürlichen Produkten (z.B. Harnstoff, Gelatine, Agar, Zellulose, etc). Die Eigenschaften und Anwendungsmöglichkeiten dieser Systeme wurden auch studiert, ebenso wie die Kontrolle der Größe und Form. So konnten wir sphärische, rohrförmige, und fiber-basierte Nanostrukturen synthetisieren, aber sogar Blätter und Holz konnten als Reaktionsmittel benutzt werden und in Nitrid und Carbid umgewandelt werden, ohne die Form zu ändern. Weil die Synthese dieser Materialien direkt gemacht werden konnte, ist prinzipiell jede weitere Modifikation, Kombination und Manipulation möglich. So können jetzt neue Systeme entworfen werden. KW - metal carbides KW - metal nitrides KW - nanostructures KW - sol-gel KW - Metallnitride KW - Metallcarbide KW - Nanostrukturen KW - Sol-Gel Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-75375 ER - TY - THES A1 - Schmidt, Lukas T1 - Aerosols and boundary layer structure over Arctic sea ice based on airborne lidar and dropsonde measurements T1 - Aerosol und Grenzschichtstruktur über arktischem Meereis anhand von Flugzeuggetragenen Lidar- und Dropsonden- Messungen N2 - The atmosphere over the Arctic Ocean is strongly influenced by the distribution of sea ice and open water. Leads in the sea ice produce strong convective fluxes of sensible and latent heat and release aerosol particles into the atmosphere. They increase the occurrence of clouds and modify the structure and characteristics of the atmospheric boundary layer (ABL) and thereby influence the Arctic climate. In the course of this study aircraft measurements were performed over the western Arctic Ocean as part of the campaign PAMARCMIP 2012 of the Alfred Wegener Institute for Polar and Marine Research (AWI). Backscatter from aerosols and clouds within the lower troposphere and the ABL were measured with the nadir pointing Airborne Mobile Aerosol Lidar (AMALi) and dropsondes were launched to obtain profiles of meteorological variables. Furthermore, in situ measurements of aerosol properties, meteorological variables and turbulence were part of the campaign. The measurements covered a broad range of atmospheric and sea ice conditions. In this thesis, properties of the ABL over Arctic sea ice with a focus on the influence of open leads are studied based on the data from the PAMARCMIP campaign. The height of the ABL is determined by different methods that are applied to dropsonde and AMALi backscatter profiles. ABL heights are compared for different flights representing different conditions of the atmosphere and of sea ice and open water influence. The different criteria for ABL height that are applied show large variation in terms of agreement among each other, depending on the characteristics of the ABL and its history. It is shown that ABL height determination from lidar backscatter by methods commonly used under mid-latitude conditions is applicable to the Arctic ABL only under certain conditions. Aerosol or clouds within the ABL are needed as a tracer for ABL height detection from backscatter. Hence an aerosol source close to the surface is necessary, that is typically found under the present influence of open water and therefore convective conditions. However it is not always possible to distinguish residual layers from the actual ABL. Stable boundary layers are generally difficult to detect. To illustrate the complexity of the Arctic ABL and processes therein, four case studies are analyzed each of which represents a snapshot of the interplay between atmosphere and underlying sea ice or water surface. Influences of leads and open water on the aerosol and clouds within the ABL are identified and discussed. Leads are observed to cause the formation of fog and cloud layers within the ABL by humidity emission. Furthermore they decrease the stability and increase the height of the ABL and consequently facilitate entrainment of air and aerosol layers from the free troposphere. N2 - Die Verteilung von Meereis und offenem Wasser hat einen starken Einfluss auf die Atmosphäre über dem arktischen Ozean. Eisrinnen (sog. Leads) verursachen konvektive Flüsse von latenter und sensibler Wärme und führen zum Eintrag von Aerosolpartikeln in die Atmosphäre. Dadurch führen sie zum vermehrten Auftreten von Wolken und modifizieren die Struktur und die Eigenschaften der atmosphärischen Grenzschicht (ABL), wodurch das arktische Klima beeinflusst wird. Im Rahmen der Messkampagne PAMARCMIP 2012 des Alfred-Wegener-Instituts (AWI) wurden als Teil dieser Arbeit über dem westlichen arktischen Ozean Flugzeugmessungen durchgeführt. Mithilfe des nach unten gerichteten Airborne Mobile Aerosol Lidar (AMALi) wurde die Rückstreuung von Aerosolen und Wolken in der unteren Troposphäre und ABL gemessen. Dropsonden wurden verwendet, um Profile meteorologischer Größen zu erhalten. Zudem wurden in situ Messungen von Aerosoleigenschaften, meteorologischen Variablen und der Turbulenz durchgeführt. In dieser Arbeit werden die Eigenschaften der ABL über arktischem Meereis basierend auf den Daten der PAMARCMIP Kampagne untersucht. Dabei liegt der Fokus auf dem Einfluss offener Leads auf die ABL. Aus den gewonnenen Dropsondendaten und AMALi Rückstreuprofilen wird die Höhe der ABL mithilfe verschiedener Methoden bestimmt. Die für verschiedene Messflüge und somit unterschiedliche atmosphärische Bedingungen sowie Meereisverteilungen berechneten ABL Höhen werden miteinander verglichen, und somit der Einfluss von offenem Wasser auf die ABL untersucht. Die verschiedenen Methoden zur Bestimmung der ABL Höhe führen zu unterschiedlichen Ergebnissen, je nach Eigenschaften der ABL und ihrer Geschichte. Es wird gezeigt, dass die Methoden für die ABL Höhen-Bestimmung aus der Lidar Rückstreuung, die gewöhnlich für die mittleren Breiten verwendet werden, nur bedingt für arktische Bedingungen geeignet ist. Um die ABL Höhe aus der Rückstreuung ableiten zu können, müssen Aerosole oder Wolken in der Grenzschicht als Tracer vorhanden sein. Dazu ist eine Aerosolquelle nahe der Oberfläche notwendig, welche typischerweise unter dem Einfluss von offenem Wasser und konvektiven Bedingungen vorliegt. Dennoch ist es nicht immer möglich, die aktuelle Grenzschicht von residualen Schichten zu unterscheiden. Stabile Grenzschichten sind im Allgemeinen schwer zu detektieren. Um die Komplexität der arktischen Grenzschicht und die beteiligten Prozesse zu veranschaulichen, werden vier Fallstudien detailliert analysiert, welche jeweils eine Momentaufnahme des Zusammenspiels von Atmosphäre und Meereis oder Wasseroberfläche darstellen. Der Einfluss von Leads und offenem Wasser auf Aerosol und Wolken in der ABL werden identifiziert und diskutiert. Die Bildung von Wolken- und Nebelschichten, verursacht durch den Feuchteeintrag über offenen Leads, wird beobachtet. Zudem verringern leads die Stabilität der ABL, führen zu einer Zunahme ihrer Höhe und begünstigen dadurch Entrainment von Luft und Aerosolschichten aus der freien Troposphäre. KW - polar KW - atmosphere KW - AMALi KW - Polar 5 KW - lead KW - polar KW - Atmosphäre KW - AMALi KW - Polar 5 KW - Eisrinne Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-75076 ER - TY - THES A1 - Meissner, Sven T1 - Implications of Microcystin Production in Microcystis aeruginosa PCC 7806 T1 - Effekte der Produktion von Microcystin in Microcystis aeruginosa PCC 7806 N2 - Cyanobacteria produce about 40 percent of the world’s primary biomass, but also a variety of often toxic peptides such as microcystin. Mass developments, so called blooms, can pose a real threat to the drinking water supply in many parts of the world. This study aimed at characterizing the biological function of microcystin production in one of the most common bloom-forming cyanobacterium Microcystis aeruginosa. In a first attempt, the effect of elevated light intensity on microcystin production and its binding to cellular proteins was studied. Therefore, conventional microcystin quantification techniques were combined with protein-biochemical methods. RubisCO, the key enzyme for primary carbon fixation was a major microcystin interaction partner. High light exposition strongly stimulated microcystin-protein interactions. Up to 60 percent of the total cellular microcystin was detected bound to proteins, i.e. inaccessible for standard quantification procedures. Underestimation of total microcystin contents when neglecting the protein fraction was also demonstrated in field samples. Finally, an immuno-fluorescence based method was developed to identify microcystin producing cyanobacteria in mixed populations. The high light induced microcystin interaction with proteins suggested an impact of the secondary metabolite on the primary metabolism of Microcystis by e.g. modulating the activity of enzymes. For addressing that question, a comprehensive GC/MS-based approach was conducted to compare the accumulation of metabolites in the wild-type of Microcystis aeruginosa PCC 7806 and the microcystin deficient ΔmcyB mutant. From all 501 detected non-redundant metabolites 85 (17 percent) accumulated significantly different in either of both genotypes upon high light exposition. Accumulation of compatible solutes in the ΔmcyB mutant suggests a role of microcystin in fine-tuning the metabolic flow to prevent stress related to excess light, high oxygen concentration and carbon limitation. Co-analysis of the widely used model cyanobacterium Synechocystis PCC 6803 revealed profound metabolic differences between species of cyanobacteria. Whereas Microcystis channeled more resources towards carbohydrate synthesis, Synechocystis invested more in amino acids. These findings were supported by electron microscopy of high light treated cells and the quantification of storage compounds. While Microcystis accumulated mainly glycogen to about 8.5 percent of its fresh weight within three hours, Synechocystis produced higher amounts of cyanophycin. The results showed that the characterization of species-specific metabolic features should gain more attention with regard to the biotechnological use of cyanobacteria. N2 - Cyanobakterien produzieren etwa 40 Prozent der primären Biomasse auf der Welt aber auch giftige Peptide wie das leberschädigende Microcystin. Massenvorkommen, so genannte Blaualgenblüten, gefährden vielerorts regelmäßig die Trinkwasserversorgung. Diese Arbeit hatte zum Ziel, den Einfluss der Microcystinproduktion auf physiologische Abläufe in dem weit verbreiteten blütenbildenden Cyanobakterium Microcystis aeruginosa zu charakterisieren. Zum einen, wurde hierfür der Einfluss der Beleuchtungsintensität auf die Produktion von Microcystin und dessen Bindung an zelluläre Proteine ermittelt. Hierzu wurden etablierte Quantifizierungstechniken mit biochemischen Methoden kombiniert. RubisCO, das Schlüsselenzym zur primären Kohlenstofffixierung, war ein Hauptinteraktionspartner von Microcystin. Hohe Beleuchtungsintensität erhöhte die Menge von an Proteine gebundenem Microcystin. Bis zu 60 Prozent des gesamten zellulären Microcystins lag an Proteine gebunden vor, d.h. es wurde durch Standardquantifizierungsmethoden nicht erfasst. Die Notwendigkeit, zur Quantifizierung des gesamten Microcystins die Proteinfraktion mit einzubeziehen, wurde auch in Freilandproben demonstriert. Die Entwicklung einer immunofluoreszenzbasierten Methode erlaubte die Unterscheidung von toxischen und nichttoxischen Microcystis-Kolonien in Freilandproben. Die starklichtinduzierte Interaktion von Microcystin mit Proteinen deutete auf einen möglichen Einfluss des Sekundärmetabolits auf den Primärstoffwechsel von Microcystis hin. Um dieser Frage nachzugehen, wurde ein umfassender GC/MS-basierter Versuch durchgeführt, um die Akkumulation von Metaboliten im Microcystin produzierenden Stamm Microcystis aeruginosa PCC 7806 und dessen microcystinfreier ΔmcyB-mutierten Variante vergleichen zu können. Es zeigte sich, dass Microcystin einen Einfluss auf die Akkumulation von 85 (17 Prozent) aller 501 detektierten Metaboliten unter erhöhter Beleuchtungsstärke hatte. Besonders die vermehrte Synthese osmotisch aktiver Substanzen in der ΔmcyB Mutante, verstanden als generelle Reaktion auf allgemeinen Stress, deutete auf eine Beteiligung von Microcystin in der metabolischen Justierung von Microcystis hin. Die Parallelanalyse des Modellstamms Synechocystis PCC 6803 offenbarte grundsätzliche metabolische Unterschiede zwischen verschiedenen Cyanobakterienspezies. Demnach produzierte Microcystis vor allem Kohlehydrate und Synechocystis eher Aminosäuren. Die GC/MS-basierten Ergebnisse wurden durch elektronmikroskopische Aufnahmen und die Quantifizierung von Speichermetaboliten gestützt. Innerhalb drei Stunden bewirkte Starklicht die Akkumulation von Glykogen in Microcystis auf ca. 8.5 Prozent des Frischgewichts, wohingegen Synechocystis mehr Cyanophycin produzierte. Die Ergebnisse zeigten, dass im Hinblick auf die biotechnologische Nutzung von Cyanobakterien, die Charakterisierung speziesspezifischer metabolischer Eigenschaften mehr Beachtung finden sollte. KW - microcystin KW - Microcystin KW - cyanobacteria KW - Cyanobakterien Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-75199 ER - TY - JOUR A1 - Metzler, Ralf A1 - Jeon, Jae-Hyung A1 - Cherstvy, Andrey G. A1 - Barkai, Eli T1 - Anomalous diffusion models and their properties BT - non-stationarity, non-ergodicity, and ageing at the centenary of single particle tracking JF - physical chemistry, chemical physics : PCCP N2 - Modern microscopic techniques following the stochastic motion of labelled tracer particles have uncovered significant deviations from the laws of Brownian motion in a variety of animate and inanimate systems. Such anomalous diffusion can have different physical origins, which can be identified from careful data analysis. In particular, single particle tracking provides the entire trajectory of the traced particle, which allows one to evaluate different observables to quantify the dynamics of the system under observation. We here provide an extensive overview over different popular anomalous diffusion models and their properties. We pay special attention to their ergodic properties, highlighting the fact that in several of these models the long time averaged mean squared displacement shows a distinct disparity to the regular, ensemble averaged mean squared displacement. In these cases, data obtained from time averages cannot be interpreted by the standard theoretical results for the ensemble averages. Here we therefore provide a comparison of the main properties of the time averaged mean squared displacement and its statistical behaviour in terms of the scatter of the amplitudes between the time averages obtained from different trajectories. We especially demonstrate how anomalous dynamics may be identified for systems, which, on first sight, appear to be Brownian. Moreover, we discuss the ergodicity breaking parameters for the different anomalous stochastic processes and showcase the physical origins for the various behaviours. This Perspective is intended as a guidebook for both experimentalists and theorists working on systems, which exhibit anomalous diffusion. KW - intermittent chaotic systems KW - Fokker-Planck equations KW - time random-walks KW - fluorescence photobleaching recovery KW - fluctuation-dissipation theorem KW - fractional dynamics approach KW - photon-counting statistics KW - weak ergodicity breaking KW - flight search patterns KW - levy flights Y1 - 2014 U6 - https://doi.org/10.1039/c4cp03465a SN - 1463-9076 SN - 1463-9084 VL - 2014 IS - 16 SP - 24128 EP - 24164 ER - TY - GEN A1 - Metzler, Ralf A1 - Jeon, Jae-Hyung A1 - Cherstvy, Andrey G. A1 - Barkai, Eli T1 - Anomalous diffusion models and their properties BT - non-stationarity, non-ergodicity, and ageing at the centenary of single particle tracking N2 - Modern microscopic techniques following the stochastic motion of labelled tracer particles have uncovered significant deviations from the laws of Brownian motion in a variety of animate and inanimate systems. Such anomalous diffusion can have different physical origins, which can be identified from careful data analysis. In particular, single particle tracking provides the entire trajectory of the traced particle, which allows one to evaluate different observables to quantify the dynamics of the system under observation. We here provide an extensive overview over different popular anomalous diffusion models and their properties. We pay special attention to their ergodic properties, highlighting the fact that in several of these models the long time averaged mean squared displacement shows a distinct disparity to the regular, ensemble averaged mean squared displacement. In these cases, data obtained from time averages cannot be interpreted by the standard theoretical results for the ensemble averages. Here we therefore provide a comparison of the main properties of the time averaged mean squared displacement and its statistical behaviour in terms of the scatter of the amplitudes between the time averages obtained from different trajectories. We especially demonstrate how anomalous dynamics may be identified for systems, which, on first sight, appear to be Brownian. Moreover, we discuss the ergodicity breaking parameters for the different anomalous stochastic processes and showcase the physical origins for the various behaviours. This Perspective is intended as a guidebook for both experimentalists and theorists working on systems, which exhibit anomalous diffusion. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 174 KW - Fokker-Planck equations KW - flight search patterns KW - fluctuation-dissipation theorem KW - fluorescence photobleaching recovery KW - fractional dynamics approach KW - intermittent chaotic systems KW - levy flights KW - photon-counting statistics KW - time random-walks KW - weak ergodicity breaking Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-74448 SP - 24128 EP - 24164 ER - TY - JOUR A1 - Wessig, Pablo A1 - Gerngroß, Maik A1 - Pape, Simon A1 - Bruhns, Philipp A1 - Weber, Jens T1 - Novel porous materials based on oligospiroketals (OSK) JF - RSC Advances : an international journal to further the chemical sciences N2 - New porous materials based on covalently connected monomers are presented. The key step of the synthesis is an acetalisation reaction. In previous years we used acetalisation reactions extensively to build up various molecular rods. Based on this approach, investigations towards porous polymeric materials were conducted by us. Here we wish to present the results of these studies in the synthesis of 1D polyacetals and porous 3D polyacetals. By scrambling experiments with 1D acetals we could prove that exchange reactions occur between different building blocks (evidenced by MALDI-TOF mass spectrometry). Based on these results we synthesized porous 3D polyacetals under the same mild conditions. KW - microporous organic polymers KW - molecular rods KW - construction KW - frameworks KW - membranes KW - sorption KW - models Y1 - 2014 U6 - https://doi.org/10.1039/c4ra04437a SN - 2046-2069 VL - 2014 IS - 4 SP - 31123 EP - 31129 ER - TY - GEN A1 - Wessig, Pablo A1 - Gerngroß, Maik A1 - Pape, Simon A1 - Bruhns, Philipp A1 - Weber, Jens T1 - Novel porous materials based on oligospiroketals (OSK) N2 - New porous materials based on covalently connected monomers are presented. The key step of the synthesis is an acetalisation reaction. In previous years we used acetalisation reactions extensively to build up various molecular rods. Based on this approach, investigations towards porous polymeric materials were conducted by us. Here we wish to present the results of these studies in the synthesis of 1D polyacetals and porous 3D polyacetals. By scrambling experiments with 1D acetals we could prove that exchange reactions occur between different building blocks (evidenced by MALDI-TOF mass spectrometry). Based on these results we synthesized porous 3D polyacetals under the same mild conditions. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 175 KW - construction KW - frameworks KW - membranes KW - microporous organic polymers KW - models KW - molecular rods KW - sorption Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-74466 SP - 31123 EP - 31129 ER - TY - JOUR A1 - Zamponi, Flavio A1 - Penfold, Thomas J. A1 - Nachtegaal, Maarten A1 - Lübcke, Andrea A1 - Rittmann, Jochen A1 - Milne, Chris J. A1 - Chergui, Majed A1 - van Bokhoven, Jeroen A. T1 - Probing the dynamics of plasmon-excited hexanethiol-capped gold nanoparticles by picosecond X-ray absorption spectroscopy JF - physical chemistry, chemical physics : PCCP N2 - Picosecond X-ray absorption spectroscopy (XAS) is used to investigate the electronic and structural dynamics initiated by plasmon excitation of 1.8 nm diameter Au nanoparticles (NPs) functionalised with 1-hexanethiol. We show that 100 ps after photoexcitation the transient XAS spectrum is consistent with an 8% expansion of the Au–Au bond length and a large increase in disorder associated with melting of the NPs. Recovery of the ground state occurs with a time constant of ∼1.8 ns, arising from thermalisation with the environment. Simulations reveal that the transient spectrum exhibits no signature of charge separation at 100 ps and allows us to estimate an upper limit for the quantum yield (QY) of this process to be <0.1. KW - TiO2 nanoparticles KW - diimine-complexes KW - electron-transfer KW - supported gold KW - visible-light KW - water KW - surface KW - reactivity KW - nanoclusters KW - excitation Y1 - 2014 U6 - https://doi.org/10.1039/c4cp03301a SN - 1463-9076 SN - 1463-9084 VL - 2014 IS - 16 SP - 23157 EP - 23163 ER - TY - GEN A1 - Zamponi, Flavio A1 - Penfold, Thomas J. A1 - Nachtegaal, Maarten A1 - Lübcke, Andrea A1 - Rittmann, Jochen A1 - Milne, Chris J. A1 - Chergui, Majed A1 - van Bokhoven, Jeroen A. T1 - Probing the dynamics of plasmon-excited hexanethiol-capped gold nanoparticles by picosecond X-ray absorption spectroscopy N2 - Picosecond X-ray absorption spectroscopy (XAS) is used to investigate the electronic and structural dynamics initiated by plasmon excitation of 1.8 nm diameter Au nanoparticles (NPs) functionalised with 1-hexanethiol. We show that 100 ps after photoexcitation the transient XAS spectrum is consistent with an 8% expansion of the Au–Au bond length and a large increase in disorder associated with melting of the NPs. Recovery of the ground state occurs with a time constant of ∼1.8 ns, arising from thermalisation with the environment. Simulations reveal that the transient spectrum exhibits no signature of charge separation at 100 ps and allows us to estimate an upper limit for the quantum yield (QY) of this process to be <0.1. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 176 KW - TiO2 nanoparticles KW - diimine-complexes KW - electron-transfer KW - excitation KW - nanoclusters KW - reactivity KW - supported gold KW - surface KW - visible-light KW - water Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-74492 SP - 23157 EP - 23163 ER - TY - JOUR A1 - Cherstvy, Andrey G. A1 - Chechkin, Aleksei V. A1 - Metzler, Ralf T1 - Particle invasion, survival, and non-ergodicity in 2D diffusion processes with space-dependent diffusivity JF - Soft matter N2 - We study the thermal Markovian diffusion of tracer particles in a 2D medium with spatially varying diffusivity D(r), mimicking recently measured, heterogeneous maps of the apparent diffusion coefficient in biological cells. For this heterogeneous diffusion process (HDP) we analyse the mean squared displacement (MSD) of the tracer particles, the time averaged MSD, the spatial probability density function, and the first passage time dynamics from the cell boundary to the nucleus. Moreover we examine the non-ergodic properties of this process which are important for the correct physical interpretation of time averages of observables obtained from single particle tracking experiments. From extensive computer simulations of the 2D stochastic Langevin equation we present an in-depth study of this HDP. In particular, we find that the MSDs along the radial and azimuthal directions in a circular domain obey anomalous and Brownian scaling, respectively. We demonstrate that the time averaged MSD stays linear as a function of the lag time and the system thus reveals a weak ergodicity breaking. Our results will enable one to rationalise the diffusive motion of larger tracer particles such as viruses or submicron beads in biological cells. KW - anomalous diffusion KW - intracellular-transport KW - adenoassociated virus KW - infection pathway KW - escherichia-coli KW - endosomal escape KW - living cells KW - trafficking KW - cytoplasm KW - models Y1 - 2014 U6 - https://doi.org/10.1039/c3sm52846d SN - 2046-2069 VL - 2014 IS - 10 SP - 1591 EP - 1601 PB - Royal Society of Chemistry ER - TY - GEN A1 - Cherstvy, Andrey G. A1 - Chechkin, Aleksei V. A1 - Metzler, Ralf T1 - Particle invasion, survival, and non-ergodicity in 2D diffusion processes with space-dependent diffusivity N2 - We study the thermal Markovian diffusion of tracer particles in a 2D medium with spatially varying diffusivity D(r), mimicking recently measured, heterogeneous maps of the apparent diffusion coefficient in biological cells. For this heterogeneous diffusion process (HDP) we analyse the mean squared displacement (MSD) of the tracer particles, the time averaged MSD, the spatial probability density function, and the first passage time dynamics from the cell boundary to the nucleus. Moreover we examine the non-ergodic properties of this process which are important for the correct physical interpretation of time averages of observables obtained from single particle tracking experiments. From extensive computer simulations of the 2D stochastic Langevin equation we present an in-depth study of this HDP. In particular, we find that the MSDs along the radial and azimuthal directions in a circular domain obey anomalous and Brownian scaling, respectively. We demonstrate that the time averaged MSD stays linear as a function of the lag time and the system thus reveals a weak ergodicity breaking. Our results will enable one to rationalise the diffusive motion of larger tracer particles such as viruses or submicron beads in biological cells. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 168 KW - adenoassociated virus KW - anomalous diffusion KW - cytoplasm KW - endosomal escape KW - escherichia-coli KW - infection pathway KW - intracellular-transport KW - living cells KW - models KW - trafficking Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-74021 IS - 168 SP - 1591 EP - 1601 ER - TY - JOUR A1 - Meyer, Sören A1 - Matissek, M. A1 - Müller, Sandra Marie A1 - Taleshi, M. S. A1 - Ebert, Franziska A1 - Francesconi, Kevin A. A1 - Schwerdtle, Tanja T1 - In vitro toxicological characterisation of three arsenic-containing hydrocarbons JF - Metallomics N2 - Arsenic-containing hydrocarbons are one group of fat-soluble organic arsenic compounds (arsenolipids) found in marine fish and other seafood. A risk assessment of arsenolipids is urgently needed, but has not been possible because of the total lack of toxicological data. In this study the cellular toxicity of three arsenic-containing hydrocarbons was investigated in cultured human bladder (UROtsa) and liver (HepG2) cells. Cytotoxicity of the arsenic-containing hydrocarbons was comparable to that of arsenite, which was applied as the toxic reference arsenical. A large cellular accumulation of arsenic, as measured by ICP-MS/MS, was observed after incubation of both cell lines with the arsenolipids. Moreover, the toxic mode of action shown by the three arsenic-containing hydrocarbons seemed to differ from that observed for arsenite. Evidence suggests that the high cytotoxic potential of the lipophilic arsenicals results from a decrease in the cellular energy level. This first in vitro based risk assessment cannot exclude a risk to human health related to the presence of arsenolipids in seafood, and indicates the urgent need for further toxicity studies in experimental animals to fully assess this possible risk. KW - cod-liver KW - human-cells KW - arsenolipids present KW - excision-repair KW - fatty-acids KW - marine oils KW - RP-HPLC KW - metabolites KW - identification KW - trivalent Y1 - 2014 U6 - https://doi.org/10.1039/c4mt00061g SN - 1756-591X SN - 1756-5901 VL - 2014 IS - 6 SP - 1023 EP - 1033 ER - TY - GEN A1 - Meyer, Sören A1 - Matissek, M. A1 - Müller, Sandra Marie A1 - Taleshi, M. S. A1 - Ebert, Franziska A1 - Francesconi, Kevin A. A1 - Schwerdtle, Tanja T1 - In vitro toxicological characterisation of three arsenic-containing hydrocarbons N2 - Arsenic-containing hydrocarbons are one group of fat-soluble organic arsenic compounds (arsenolipids) found in marine fish and other seafood. A risk assessment of arsenolipids is urgently needed, but has not been possible because of the total lack of toxicological data. In this study the cellular toxicity of three arsenic-containing hydrocarbons was investigated in cultured human bladder (UROtsa) and liver (HepG2) cells. Cytotoxicity of the arsenic-containing hydrocarbons was comparable to that of arsenite, which was applied as the toxic reference arsenical. A large cellular accumulation of arsenic, as measured by ICP-MS/MS, was observed after incubation of both cell lines with the arsenolipids. Moreover, the toxic mode of action shown by the three arsenic-containing hydrocarbons seemed to differ from that observed for arsenite. Evidence suggests that the high cytotoxic potential of the lipophilic arsenicals results from a decrease in the cellular energy level. This first in vitro based risk assessment cannot exclude a risk to human health related to the presence of arsenolipids in seafood, and indicates the urgent need for further toxicity studies in experimental animals to fully assess this possible risk. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 170 KW - cod-liver KW - human-cells KW - arsenolipids present KW - excision-repair KW - fatty-acids KW - marine oils KW - RP-HPLC KW - metabolites KW - identification KW - trivalent Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-74201 SP - 1023 EP - 1033 ER - TY - JOUR A1 - Grözinger, Karl E. T1 - Jüdische Mystik JF - Handbuch zur Geschichte der Juden in Europa N2 - Das Handbuch umreißt das gesamte Spektrum der 2000jährigen Geschichte der Juden auf europäischem Boden. Wissenschaftler aus Europa und den USA haben ihre Forschungsergebnisse allgemein verständlich aufbereitet mit dem Ziel, das Leben und Wirken der Juden, aber auch die ihnen entgegengebrachte Intoleranz und deren Ursachen aufzuzeigen. Der erste Teil konzentriert sich systematisch auf die Länder und Regionen, in denen Juden siedelten bzw. nach Vertreibungen aus anderen Staaten Aufnahme fanden. Es wird sowohl auf die innere Entwicklung der jüdischen Gemeinden als auch auf die Beziehungen zwischen Juden und der sie umgebenden andersgläubigen Gesellschaft eingegangen. Der zweite Teil behandelt themenspezifische Schwerpunkte. Gemeindeleben und Religion, Familie und Stellung der Frau, kulturelle und geistige Entwicklung, aber auch Judenfeindschaft der sie umgebenden Gesellschaft – vom Antijudaismus bis zur Shoa und zum Antisemitismus der Gegenwart – werden in großangelegten Beiträgen dargelegt. Y1 - 2014 SN - 978-3-86312-363-5 SP - 127 EP - 137 PB - Primus Verlag CY - Frankfurt a.M. ER - TY - JOUR A1 - Unterberg, Marlies A1 - Leffers, Larissa A1 - Hübner, Florian A1 - Humpf, Hans-Ulrich A1 - Lepikhov, Konstantin A1 - Walter, Jörn A1 - Ebert, Franziska A1 - Schwerdtle, Tanja T1 - Toxicity of arsenite and thio-DMAV after long-term (21 days) incubation of human urothelial cells: cytotoxicity, genotoxicity and epigenetics JF - Toxicology Research N2 - This study aims to further mechanistically understand toxic modes of action after chronic inorganic arsenic exposure. Therefore long-term incubation studies in cultured cells were carried out, to display chronically attained changes, which cannot be observed in the generally applied in vitro short-term incubation studies. Particularly, the cytotoxic, genotoxic and epigenetic effects of an up to 21 days incubation of human urothelial (UROtsa) cells with pico- to nanomolar concentrations of iAsIII and its metabolite thio-DMAV were compared. After 21 days of incubation, cytotoxic effects were strongly enhanced in the case of iAsIII and might partly be due to glutathione depletion and genotoxic effects on the chromosomal level. These results are in strong contrast to cells exposed to thio-DMAV. Thus, cells seemed to be able to adapt to this arsenical, as indicated among others by an increase in the cellular glutathione level. Most interestingly, picomolar concentrations of both iAsIII and thio-DMAV caused global DNA hypomethylation in UROtsa cells, which was quantified in parallel by 5-medC immunostaining and a newly established, reliable, high resolution mass spectrometry (HRMS)-based test system. This is the first time that epigenetic effects are reported for thio-DMAV; iAsIII induced epigenetic effects occur in at least 8000 fold lower concentrations as reported in vitro before. The fact that both arsenicals cause DNA hypomethylation at really low, exposure-relevant concentrations in human urothelial cells suggests that this epigenetic effect might contribute to inorganic arsenic induced carcinogenicity, which for sure has to be further investigated in future studies. KW - induced malignant-transformation KW - genomic dna methylation KW - vitro toxicological characterization KW - thio-dimethylarsinic acid KW - bladder-cancer KW - methyltransferases dnmt3a KW - cytosine methylation KW - carcinogen exposure KW - mass-spectrometry KW - gene-expression Y1 - 2014 SN - 2045-4538 SN - 2045-452X VL - 3 IS - 6 SP - 456 EP - 464 PB - Royal Society of Chemistry CY - Cambridge ER - TY - GEN A1 - Unterberg, Marlies A1 - Leffers, Larissa A1 - Hübner, Florian A1 - Humpf, Hans-Ulrich A1 - Lepikhov, Konstantin A1 - Walter, Jörn A1 - Ebert, Franziska A1 - Schwerdtle, Tanja T1 - Toxicity of arsenite and thio-DMAV after long-term (21 days) incubation of human urothelial cells: cytotoxicity, genotoxicity and epigenetics N2 - This study aims to further mechanistically understand toxic modes of action after chronic inorganic arsenic exposure. Therefore long-term incubation studies in cultured cells were carried out, to display chronically attained changes, which cannot be observed in the generally applied in vitro short-term incubation studies. Particularly, the cytotoxic, genotoxic and epigenetic effects of an up to 21 days incubation of human urothelial (UROtsa) cells with pico- to nanomolar concentrations of iAsIII and its metabolite thio-DMAV were compared. After 21 days of incubation, cytotoxic effects were strongly enhanced in the case of iAsIII and might partly be due to glutathione depletion and genotoxic effects on the chromosomal level. These results are in strong contrast to cells exposed to thio-DMAV. Thus, cells seemed to be able to adapt to this arsenical, as indicated among others by an increase in the cellular glutathione level. Most interestingly, picomolar concentrations of both iAsIII and thio-DMAV caused global DNA hypomethylation in UROtsa cells, which was quantified in parallel by 5-medC immunostaining and a newly established, reliable, high resolution mass spectrometry (HRMS)-based test system. This is the first time that epigenetic effects are reported for thio-DMAV; iAsIII induced epigenetic effects occur in at least 8000 fold lower concentrations as reported in vitro before. The fact that both arsenicals cause DNA hypomethylation at really low, exposure-relevant concentrations in human urothelial cells suggests that this epigenetic effect might contribute to inorganic arsenic induced carcinogenicity, which for sure has to be further investigated in future studies. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 178 KW - induced malignant-transformation KW - genomic dna methylation KW - vitro toxicological characterization KW - thio-dimethylarsinic acid KW - bladder-cancer KW - methyltransferases dnmt3a KW - cytosine methylation KW - carcinogen exposure KW - mass-spectrometry KW - gene-expression Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-76239 SP - 456 EP - 464 ER - TY - JOUR A1 - Palyulin, Vladimir V. A1 - Ala-Nissila, Tapio A1 - Metzler, Ralf ED - Metzler, Ralf T1 - Polymer translocation: the first two decades and the recent diversification JF - Soft matter N2 - Probably no other field of statistical physics at the borderline of soft matter and biological physics has caused such a flurry of papers as polymer translocation since the 1994 landmark paper by Bezrukov, Vodyanoy, and Parsegian and the study of Kasianowicz in 1996. Experiments, simulations, and theoretical approaches are still contributing novel insights to date, while no universal consensus on the statistical understanding of polymer translocation has been reached. We here collect the published results, in particular, the famous–infamous debate on the scaling exponents governing the translocation process. We put these results into perspective and discuss where the field is going. In particular, we argue that the phenomenon of polymer translocation is non-universal and highly sensitive to the exact specifications of the models and experiments used towards its analysis. KW - solid-state nanopores KW - single-stranded-dna KW - posttranslational protein translocation KW - anomalous diffusion KW - monte-carlo KW - structured polynucleotides KW - dynamics simulation KW - equation approach KW - osmotic-pressure KW - membrane channel Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-76266 SN - 1744-683X VL - 45 IS - 10 SP - 9016 EP - 9037 PB - the Royal Society of Chemistry CY - Cambridge ER - TY - GEN A1 - Palyulin, Vladimir V. A1 - Ala-Nissila, Tapio A1 - Metzler, Ralf T1 - Polymer translocation: the first two decades and the recent diversification N2 - Probably no other field of statistical physics at the borderline of soft matter and biological physics has caused such a flurry of papers as polymer translocation since the 1994 landmark paper by Bezrukov, Vodyanoy, and Parsegian and the study of Kasianowicz in 1996. Experiments, simulations, and theoretical approaches are still contributing novel insights to date, while no universal consensus on the statistical understanding of polymer translocation has been reached. We here collect the published results, in particular, the famous–infamous debate on the scaling exponents governing the translocation process. We put these results into perspective and discuss where the field is going. In particular, we argue that the phenomenon of polymer translocation is non-universal and highly sensitive to the exact specifications of the models and experiments used towards its analysis. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 179 KW - solid-state nanopores KW - single-stranded-dna KW - posttranslational protein translocation KW - anomalous diffusion KW - monte-carlo KW - structured polynucleotides KW - dynamics simulation KW - equation approach KW - osmotic-pressure KW - membrane channel Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-76287 SP - 9016 EP - 9037 ER - TY - JOUR A1 - Jeon, Jae-Hyung A1 - Chechkin, Aleksei V. A1 - Metzler, Ralf T1 - Scaled Brownian motion: a paradoxical process with a time dependent diffusivity for the description of anomalous diffusion JF - Physical chemistry, chemical physics : PCCP N2 - Anomalous diffusion is frequently described by scaled Brownian motion (SBM){,} a Gaussian process with a power-law time dependent diffusion coefficient. Its mean squared displacement is ?x2(t)? [similar{,} equals] 2K(t)t with K(t) [similar{,} equals] t[small alpha]-1 for 0 < [small alpha] < 2. SBM may provide a seemingly adequate description in the case of unbounded diffusion{,} for which its probability density function coincides with that of fractional Brownian motion. Here we show that free SBM is weakly non-ergodic but does not exhibit a significant amplitude scatter of the time averaged mean squared displacement. More severely{,} we demonstrate that under confinement{,} the dynamics encoded by SBM is fundamentally different from both fractional Brownian motion and continuous time random walks. SBM is highly non-stationary and cannot provide a physical description for particles in a thermalised stationary system. Our findings have direct impact on the modelling of single particle tracking experiments{,} in particular{,} under confinement inside cellular compartments or when optical tweezers tracking methods are used. KW - single-particle tracking KW - living cells KW - random-walks KW - subdiffusion KW - dynamics KW - nonergodicity KW - coefficients KW - transport KW - membrane KW - behavior Y1 - 2014 U6 - https://doi.org/10.1039/C4CP02019G VL - 30 IS - 16 SP - 15811 EP - 15817 PB - The Royal Society of Chemistry CY - Cambridge ER - TY - THES A1 - Sachse, Rita T1 - Biological membranes in cell-free systems BT - characterisation and functionalisation of spodoptera frugiperda derived microsomes Y1 - 2014 ER - TY - JOUR A1 - Berner, Elisabeth T1 - Von der Schwierigkeit, einer Stadt eine Kunsthalle zu schenken BT - Strategien der Emotionalisierung in einem aktuellen kommunalpolitischen Diskurs JF - Emotionalität im Text Y1 - 2014 SN - 978-3-95809-506-9 SN - 1430-4139 IS - 85 SP - 231 EP - 248 PB - Stauffenburg CY - Tübingen ER - TY - JOUR A1 - Teske, Daniel T1 - Geocoder accuracy ranking JF - Process design for natural scientists: an agile model-driven approach N2 - Finding an address on a map is sometimes tricky: the chosen map application may be unfamiliar with the enclosed region. There are several geocoders on the market, they have different databases and algorithms to compute the query. Consequently, the geocoding results differ in their quality. Fortunately the geocoders provide a rich set of metadata. The workflow described in this paper compares this metadata with the aim to find out which geocoder is offering the best-fitting coordinate for a given address. Y1 - 2014 SN - 978-3-662-45005-5 SN - 1865-0929 IS - 500 SP - 161 EP - 174 PB - Springer CY - Berlin ER - TY - JOUR A1 - Sens, Henriette T1 - Web-Based map generalization tools put to the test: a jABC workflow JF - Process Design for Natural Scientists: an agile model-driven approach N2 - Geometric generalization is a fundamental concept in the digital mapping process. An increasing amount of spatial data is provided on the web as well as a range of tools to process it. This jABC workflow is used for the automatic testing of web-based generalization services like mapshaper.org by executing its functionality, overlaying both datasets before and after the transformation and displaying them visually in a .tif file. Mostly Web Services and command line tools are used to build an environment where ESRI shapefiles can be uploaded, processed through a chosen generalization service and finally visualized in Irfanview. Y1 - 2014 SN - 978-3-662-45005-5 SN - 1865-0929 IS - 500 SP - 175 EP - 185 PB - Springer CY - Berlin ER - TY - JOUR A1 - Noack, Franziska T1 - CREADED: Colored-Relief application for digital elevation data JF - Process design for natural scientists: an agile model-driven approach N2 - In the geoinformatics field, remote sensing data is often used for analyzing the characteristics of the current investigation area. This includes DEMs, which are simple raster grids containing grey scales representing the respective elevation values. The project CREADED that is presented in this paper aims at making these monochrome raster images more significant and more intuitively interpretable. For this purpose, an executable interactive model for creating a colored and relief-shaded Digital Elevation Model (DEM) has been designed using the jABC framework. The process is based on standard jABC-SIBs and SIBs that provide specific GIS functions, which are available as Web services, command line tools and scripts. Y1 - 2014 SN - 978-3-662-45005-5 SN - 1865-0929 IS - 500 SP - 186 EP - 199 PB - Springer CY - Berlin ER - TY - JOUR A1 - Respondek, Tobias T1 - A workflow for computing potential areas for wind turbines JF - Process design for natural scientists: an agile model-driven approach N2 - This paper describes the implementation of a workflow model for service-oriented computing of potential areas for wind turbines in jABC. By implementing a re-executable model the manual effort of a multi-criteria site analysis can be reduced. The aim is to determine the shift of typical geoprocessing tools of geographic information systems (GIS) from the desktop to the web. The analysis is based on a vector data set and mainly uses web services of the “Center for Spatial Information Science and Systems” (CSISS). This paper discusses effort, benefits and problems associated with the use of the web services. Y1 - 2014 SN - 978-3-662-45005-5 IS - 500 SP - 200 EP - 215 PB - Springer CY - Berlin ER - TY - JOUR A1 - Scheele, Lasse T1 - Location analysis for placing artificial reefs JF - Process design for natural scientists: an agile model-driven approach N2 - Location analyses are among the most common tasks while working with spatial data and geographic information systems. Automating the most frequently used procedures is therefore an important aspect of improving their usability. In this context, this project aims to design and implement a workflow, providing some basic tools for a location analysis. For the implementation with jABC, the workflow was applied to the problem of finding a suitable location for placing an artificial reef. For this analysis three parameters (bathymetry, slope and grain size of the ground material) were taken into account, processed, and visualized with the The Generic Mapping Tools (GMT), which were integrated into the workflow as jETI-SIBs. The implemented workflow thereby showed that the approach to combine jABC with GMT resulted in an user-centric yet user-friendly tool with high-quality cartographic outputs. Y1 - 2014 SN - 978-3-662-45005-5 SN - 1865-0929 IS - 500 SP - 216 EP - 228 PB - Springer CY - Berlin ER - TY - JOUR A1 - Kind, Josephine T1 - Creation of topographic maps JF - Process design for natural scientists: an agile model-driven approach N2 - Location analyses are among the most common tasks while working with spatial data and geographic information systems. Automating the most frequently used procedures is therefore an important aspect of improving their usability. In this context, this project aims to design and implement a workflow, providing some basic tools for a location analysis. For the implementation with jABC, the workflow was applied to the problem of finding a suitable location for placing an artificial reef. For this analysis three parameters (bathymetry, slope and grain size of the ground material) were taken into account, processed, and visualized with the The Generic Mapping Tools (GMT), which were integrated into the workflow as jETI-SIBs. The implemented workflow thereby showed that the approach to combine jABC with GMT resulted in an user-centric yet user-friendly tool with high-quality cartographic outputs. Y1 - 2014 SN - 978-3-662-45005-5 IS - 500 SP - 229 EP - 238 PB - Springer CY - Berlin ER - TY - JOUR A1 - Holler, Robin T1 - GraffDok - a graffiti documentation application JF - Process design for natural scientists: an agile model-driven approach N2 - GraffDok is an application helping to maintain an overview over sprayed images somewhere in a city. At the time of writing it aims at vandalism rather than at beautiful photographic graffiti in an underpass. Looking at hundreds of tags and scribbles on monuments, house walls, etc. it would be interesting to not only record them in writing but even make them accessible electronically, including images. GraffDok’s workflow is simple and only requires an EXIF-GPS-tagged photograph of a graffito. It automatically determines its location by using reverse geocoding with the given GPS-coordinates and the Gisgraphy WebService. While asking the user for some more meta data, GraffDok analyses the image in parallel with this and tries to detect fore- and background – before extracting the drawing lines and make them stand alone. The command line based tool ImageMagick is used here as well as for accessing EXIF data. Any meta data is written to csv-files, which will stay easily accessible and can be integrated in TeX-files as well. The latter ones are converted to PDF at the end of the workflow, containing a table about all graffiti and a summary for each – including the generated characteristic graffiti pattern image. Y1 - 2014 SN - 978-3-662-45005-5 SN - 1865-0929 IS - 500 SP - 239 EP - 251 PB - Springer CY - Berlin ER - TY - JOUR A1 - Kosta, Peter T1 - Die Tschechoslowakai seit 1918 : der rechtliche Status der Juden nach der Verfassung und dem Sprachengesetz von 1920 JF - Handbuch zur Geschichte der Juden in Europa Y1 - 2014 SN - 978-3-86312-363-5 SP - 143 EP - 150 PB - Primus Verlag CY - Frankfurt a.M. ER - TY - JOUR A1 - Franz, Norbert P. A1 - Jilge, Wilfried T1 - Rußland, Ukraine, Weißrußland, Baltikum (Lettland, Estland) JF - Handbuch zur Geschichte der Juden in Europa Y1 - 2014 SN - 978-3-86312-363-5 SP - 167 EP - 227 PB - Primus Verlag CY - Frankfurt a.M. ER - TY - JOUR A1 - Reso, Judith ED - Lambrecht, Anna-Lena ED - Margaria, Tiziana T1 - Protein Classification Workflow JF - Process Design for Natural Scientists: an agile model-driven approach N2 - The protein classification workflow described in this report enables users to get information about a novel protein sequence automatically. The information is derived by different bioinformatic analysis tools which calculate or predict features of a protein sequence. Also, databases are used to compare the novel sequence with known proteins. Y1 - 2014 SN - 978-3-662-45005-5 SN - 1865-0929 IS - 500 SP - 65 EP - 72 PB - Springer Verlag CY - Berlin ER - TY - JOUR A1 - Schulze, Gunnar T1 - Workflow for rapid metagenome analysis JF - Process design for natural scientists: an agile model-driven approach N2 - Analyses of metagenomes in life sciences present new opportunities as well as challenges to the scientific community and call for advanced computational methods and workflows. The large amount of data collected from samples via next-generation sequencing (NGS) technologies render manual approaches to sequence comparison and annotation unsuitable. Rather, fast and efficient computational pipelines are needed to provide comprehensive statistics and summaries and enable the researcher to choose appropriate tools for more specific analyses. The workflow presented here builds upon previous pipelines designed for automated clustering and annotation of raw sequence reads obtained from next-generation sequencing technologies such as 454 and Illumina. Employing specialized algorithms, the sequence reads are processed at three different levels. First, raw reads are clustered at high similarity cutoff to yield clusters which can be exported as multifasta files for further analyses. Independently, open reading frames (ORFs) are predicted from raw reads and clustered at two strictness levels to yield sets of non-redundant sequences and ORF families. Furthermore, single ORFs are annotated by performing searches against the Pfam database Y1 - 2014 SN - 978-3-662-45005-5 SN - 1865-0929 IS - 500 SP - 88 EP - 100 PB - Springer CY - Berlin ER - TY - JOUR A1 - Vierheller, Janine ED - Lambrecht, Anna-Lena ED - Margaria, Tiziana T1 - Exploratory Data Analysis JF - Process Design for Natural Scientists: an agile model-driven approach N2 - In bioinformatics the term exploratory data analysis refers to different methods to get an overview of large biological data sets. Hence, it helps to create a framework for further analysis and hypothesis testing. The workflow facilitates this first important step of the data analysis created by high-throughput technologies. The results are different plots showing the structure of the measurements. The goal of the workflow is the automatization of the exploratory data analysis, but also the flexibility should be guaranteed. The basic tool is the free software R. Y1 - 2014 SN - 978-3-662-45005-5 SN - 1865-0929 IS - 500 SP - 110 EP - 126 PB - Axel Springer Verlag CY - Berlin ER - TY - JOUR A1 - Schütt, Christine T1 - Identification of differentially expressed genes JF - Process design for natural scientists: an agile model-driven approach N2 - With the jABC it is possible to realize workflows for numerous questions in different fields. The goal of this project was to create a workflow for the identification of differentially expressed genes. This is of special interest in biology, for it gives the opportunity to get a better insight in cellular changes due to exogenous stress, diseases and so on. With the knowledge that can be derived from the differentially expressed genes in diseased tissues, it becomes possible to find new targets for treatment. Y1 - 2014 SN - 978-3-662-45005-5 SN - 1865-0929 IS - 500 SP - 127 EP - 139 PB - Springer CY - Berlin ER - TY - JOUR A1 - Kuntzsch, Christian T1 - Visualization of data transfer paths JF - Process design for natural scientists: an agile model-driven approach N2 - A workflow for visualizing server connections using the Google Maps API was built in the jABC. It makes use of three basic services: An XML-based IP address geolocation web service, a command line tool and the Static Maps API. The result of the workflow is an URL leading to an image file of a map, showing server connections between a client and a target host. Y1 - 2014 SN - 978-3-662-45005-5 SN - 1865-0929 IS - 500 SP - 140 EP - 148 PB - Springer CY - Berlin ER - TY - JOUR A1 - Hibbe, Marcel ED - Lambrecht, Anna-Lena ED - Margaria, Tiziana T1 - Spotlocator - Guess Where the Photo Was Taken! JF - Process Design for Natural Scientists: an agile model-driven approach N2 - Spotlocator is a game wherein people have to guess the spots of where photos were taken. The photos of a defined area for each game are from panoramio.com. They are published at http://spotlocator. drupalgardens.com with an ID. Everyone can guess the photo spots by sending a special tweet via Twitter that contains the hashtag #spotlocator, the guessed coordinates and the ID of the photo. An evaluation is published for all tweets. The players are informed about the distance to the real photo spots and the positions are shown on a map. Y1 - 2014 SN - 978-3-662-45005-5 SN - 1865-0929 IS - 500 SP - 149 EP - 160 PB - Springer Verlag CY - Berlin ER - TY - JOUR A1 - Blaese, Leif T1 - Data mining for unidentified protein squences JF - Process design for natural scientists: an agile model-driven approach N2 - Through the use of next generation sequencing (NGS) technology, a lot of newly sequenced organisms are now available. Annotating those genes is one of the most challenging tasks in sequence biology. Here, we present an automated workflow to find homologue proteins, annotate sequences according to function and create a three-dimensional model. Y1 - 2014 SN - 978-3-662-45005-5 SN - 1865-0929 IS - 500 SP - 73 EP - 87 PB - Springer CY - Berlin ER - TY - JOUR A1 - Lis, Monika ED - Lambrecht, Anna-Lena ED - Margaria, Tiziana T1 - Constructing a Phylogenetic Tree JF - Process Design for Natural Scientists: an agile model-driven approach N2 - In this project I constructed a workflow that takes a DNA sequence as input and provides a phylogenetic tree, consisting of the input sequence and other sequences which were found during a database search. In this phylogenetic tree the sequences are arranged depending on similarities. In bioinformatics, constructing phylogenetic trees is often used to explore the evolutionary relationships of genes or organisms and to understand the mechanisms of evolution itself. Y1 - 2014 SN - 978-3-662-45005-5 SN - 1865-0929 IS - 500 SP - 101 EP - 109 PB - Springer Verlag CY - Berlin ER - TY - CHAP A1 - Haßler, Gerda T1 - Unità e diversità delle teorie linguistiche del XVII e del XVIII secolo T2 - Linguaggio, filosofia, fisiologia nell’età moderna : Atti del Convegno Roma 23-25 gennaio 2014 (ILIESI digitale. Ricerche filosofiche e lessicali ; 1) N2 - Uno sguardo che renda omogenee le teorie della lingua relative al XVII e al XVIII secolo non può cogliere che a grandi linee la realtà delle concezioni della lingua abbracciate in questo periodo. Il riconoscimento di una teoria cartesiana della lingua come la spiegazione indifferenziata degli sviluppi conseguenti il passaggio da visioni razionalistiche a concezioni orientate ai sensi sono risultato di tale omogeneizzazione, un processo che contempla la realtà solo in parte. Il pensiero linguistico era contrassegnato da un misto di forme di riflessione di carattere narrativo e di tipo concettuale-razionale che si completavano in modo reciproco. Se l’approccio concettuale tentava di rilevare le proprietà fondamentali della lingua e ordinarle razionalmente, le forme narrative della riflessione linguistica non si rivolgevano alla lingua in quanto oggetto concettuale. Piuttosto la rappresentavano come oggetto da comprendere. Gli approcci narrativi e concettuali alla lingua prevedono differenze discorsive nelle impostazioni teorico-linguistiche. Anche lo stampo del pensiero teoretico-linguistico contribuisce, attraverso tradizioni differenti, alla molteplicità delle vedute teoretico-linguistiche. Per tradizioni intendiamo posizioni dominanti nella riflessione metalinguistica, presenti in contesti regionali, che possono differenziarsi da altre tradizioni. Ad ogni modo, anche il ritardato sviluppo o la ricezione di teorie linguistiche può condurre a differenze caratteristiche. Le teorie linguistiche dell´Illuminismo furono per esempio recepite in Spagna più tardi che in altri Paesi europei. Ciò condusse all’accettazione sincronica di elementi teorici relativi a teorie diverse e consecutive. Se si concentra l’attenzione al di fuori dell’Europa si verrà attratti dallo sviluppo degli approcci analoghi alla riflessione linguistica che trovarono sviluppo in Cina all’inizio del XX secolo. Unità e diversità sono tuttavia rintracciabili non solo sul piano della conoscenza metalinguistica ma anche sul piano dell’oggetto. Una sfida per la descrizione della lingua orientata alla tradizione greco-latina era rappresentata dalle lingue indigene con le quali si stava iniziando ad entrare in contatto attraverso i viaggi di scoperta e in seguito all’inizio del colonialismo. Da affiancare alla comunicazione esogena della trasmissione metalinguistica dei rapporti nell’ambito delle lingue europee sono presenti anche approcci per una percezione della specificità categoriale delle lingue americane. Sebbene in alcuni casi non verranno riconosciute le giuste categorizzazioni per le lingue descritte, per lo meno verrà assodato che le categorie rese note dalla grammatica latina non sussistono. Nella ricerca degli ultimi decenni, la rappresentazione di un paradigma della filosofia della lingua del XVII e del XVIII secolo che postordini e subordini universalmente la molteplicità delle lingue a strutture valide di pensiero e che prescriva per la riflessione linguistica categorie fisse di una grammatica generale strettamente orientata alla logica razionalistica è stata più volte relativizzata. In quanto connessa con la fondatezza dell’unità e con l’inalterabilità del genere umano a seconda di spazio e tempo, la tesi che le lingue nella loro natura molteplice possano esistere solo alle dipendenze di una struttura universale del pensiero si lasciava catalogare tra quelle posizioni paradigmatiche sussistenti nell’ambito della filosofia della lingua di allora. Attraverso la conoscenza dell’origine storica dell’evoluzione dell’uomo, di tutti i suoi stili di vita e forme di comunicazione, assume rilievo un’altra posizione paradigmatica che attribuisce alla lingua un influsso formativo sul pensiero. Attraverso la differenziazione ideologico-filosofica e la specificità nazionale delle sue tesi relative alla lingua in generale e alle lingue storiche in particolare la visione secolarizzata dell’uomo e della società elaborata all’apice dell’Illuminismo si associava allo sviluppo corrispettivo e al cambiamento delle posizioni teorico-linguistiche. Con la proclamazione della lingua e del pensiero come risultati di un lungo sviluppo corrispettivo nella storia dell’umanità viene assegnato nuovo valore alle prese di posizione sulla natura e sull’origine della lingua. Y1 - 2015 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-82010 UR - http://www.iliesi.cnr.it/pubblicazioni/Ricerche-01-Marras_Schino.pdf SN - 978-88-9782-803-7 SN - 2464-8698 SP - 273 EP - 296 PB - Istituto per il Lessica Intellettuale Europeo e Storia delle Idee CY - Rom ER - TY - THES A1 - Eggers, Ute T1 - Environmental impacts on white stork (Ciconia ciconia) breeding success BT - a long-term study with a focus on effects of weather conditions at the breeding grounds Y1 - 2014 ER - TY - THES A1 - Gaßmöller, René T1 - The interaction of subducted slabs and plume generation zones in geodynamic models Y1 - 2014 ER - TY - THES A1 - Soja, Aleksandra Maria T1 - Transcriptomic and metabolomic analysis of Arabidopsis thaliana during abiotic stress Y1 - 2014 ER - TY - THES A1 - Brem, Patrick T1 - Compact objects in dense astrophysical environments BT - numerical simulations and implications for gravitational wave astronomy Y1 - 2014 ER - TY - THES A1 - Müller, Jörg T1 - Response of bryophyte diversity to land-use and management in forest and grassland habitats Y1 - 2014 ER - TY - THES A1 - Dirakis, Alexis T1 - Grundlagen und Grenzen des Sozialen T1 - Fondements et limites anthropologiques du social BT - synthèse et développement sociologique de l'anthropologie philosophique de Helmuth Plessner BT - Zusammenfassung und soziologische Entwicklung der philosophischen Anthropologie von Helmuth Plessner N2 - Cette recherche a pour objet l'articulation entre les dimensions anthropologiques et sociologiques de l'anthropologie philosophique de Helmuth Plessner (1892-1985). Elles procèdent selon trois axes. Je m'efforce (1) d'offrir une synthèse de l'anthropologie philosophique plessnerienne afin (2) de reconstituer les conditions de possibilité du social au stade humain de l'organique. Le troisième axe (3) correspond, enfin, à l'analyse des limites structurelles du social à partir de ses deux dimensions constitutives : l'individuel (limites ontogénétiques, comportementales et inter-personnelles) et le collectif (limites culturelles, intra- et inter-culturelles). N2 - This research focuses on the relationship between anthropological and sociological dimensions of philosophical anthropology of Helmuth Plessner (1892-1985). It proceeds along three axes. This research try to (1) provide a synthesis of plessnerian philosophical anthropology (2) in order to reconstruct the social conditions of possibility of the human stage of the organic ("die menschliche Stufe des Organischen"). Finally, the third axis (3) is dedicated to the analysis of the structural limits ("Grenzen") of the social from two of its constituent dimensions: the individual limits (as ontogenetic, behavioral and interpersonal limitations) and the collective limits (as cultural, intra- and inter-cultural boundaries). Y1 - 2014 ER -