TY  - JOUR
A1  - Daskalow, Katjana
A1  - Boisguerin, Prisca
A1  - Jandrig, Burkhard
A1  - van Landeghem, Frank K. H.
A1  - Volkmer, Rudolf
A1  - Micheel, Burkhard
A1  - Schenk, Jörg A.
T1  - Generation of an antibody against the protein phosphatase 1 inhibitor KEPI and characterization of the epitope
N2  - A monoclonal antibody against the potential tumor suppressor kinase-enhanced protein phosphatase 1 (PP1) inhibitor KEPI (PPP1R14C) was generated and characterized. Human KEPI was expressed in Escherichia coli and used to immunize Balb/c mice. Using hybridoma technology, one clone, G18AF8, was isolated producing antibodies which bound specifically to the KEPI protein in ELISA, immunoblotting and flow cytometry. The antibody was also successfully applied to stain KEPI protein in paraffin sections of human brain. The epitope was mapped using peptide array technology and confirmed as GARVFFQSPR. This corresponds to the N-terminal region of KEPI. Amino acid substitution analysis revealed that two residues, F and Q, are essential for binding. Affinity of binding was determined by competitive ELISA as 1 mu M. In Western blot assays testing G18AF8 antibody on brain samples of several species, reactivity with hamster, rat and chicken samples was found, suggesting a broad homology of this KEPI epitope in vertebrates. This antibody could be used in expression studies at the protein level e.g. in tumor tissues.
Y1  - 2010
UR  - http://ar.iiarjournals.org/
SN  - 0250-7005
ER  - 
TY  - JOUR
A1  - Gedvilaite, Alma
A1  - Frömmel, C.
A1  - Sasnauskas, K.
A1  - Micheel, Burkhard
A1  - Özel, M.
A1  - Behrsing, Olaf
A1  - Staniulis, J.
A1  - Jandrig, Burkhard
A1  - Scherneck, Siegfried
A1  - Ulrich, R.
T1  - Formation of immunogenic virus-like particles by inserting epitopes into surface-exposed regions of hamster polyomavirus major capsid protein
Y1  - 2000
ER  - 
TY  - JOUR
A1  - Jandrig, Burkhard
A1  - Seitz, Susanne
A1  - Hinzmann, Bernd
A1  - Arnold, Wolfgang
A1  - Micheel, Burkhard
A1  - Koelble, Konrad
A1  - Siebert, Reiner
A1  - Schwartz, Arnfried
A1  - Ruecker, Karin
A1  - Schlag, Peter M.
A1  - Scherneck, Siegfried
A1  - Rosenthal, Andra
T1  - ST18 is a breast cancer tumor suppressor gene at human chromosome 8q11.2
N2  - We have identified a gene, ST18 (suppression of tumorigenicity 18, breast carcinoma, zinc-finger protein), within a frequent imbalanced region of chromosome 8q11 as a breast cancer tumor suppressor gene. The ST18 gene encodes a zinc-finger DNA-binding protein with six fingers of the C2HC type (configuration Cys-X5-Cys-X12-His-X4-Cys) and an SMC domain. ST18 has the potential to act as transcriptional regulator. ST18 is expressed in a number of normal tissues including mammary epithelial cells although the level of expression is quite low. In breast cancer cell lines and the majority of primary breast tumors, ST18 mRNA is significantly downregulated. A 160 bp region within the promoter of the ST18 gene is hypermethylated in about 80% of the breast cancer samples and in the majority of breast cancer cell lines. The strong correlation between ST18 promoter hypermethylation and loss of ST18 expression in tumor cells suggests that this epigenetic mechanism is responsible for tumor-specific downregulation. We further show that ectopic ST18 expression in MCF-7 breast cancer cells strongly inhibits colony formation in soft agar and the formation of tumors in a xenograft mouse model
Y1  - 2004
UR  - http://www.nature.com/cgi-taf/DynaPage.taf?file=/onc/journal/v23/n57/abs/ 1208131a.html&dynoptions=doi1113987275
ER  - 
TY  - JOUR
A1  - Lawatscheck, Robert
A1  - Aleksaite, Egle
A1  - Schenk, Jörg A.
A1  - Micheel, Burkhard
A1  - Jandrig, Burkhard
A1  - Holland, Gudrun
A1  - Sasnauskas, Kestutius
A1  - Gedvilaite, Alma
A1  - Ulrich, Rainer Günter
T1  - Chimeric polyomavirus-derived virus-like particles : the immunogenicity of an inserted peptide applied without adjuvant to mice depends on its insertion site and its flanking linker sequence
N2  - We inserted the sequence of the carcinoembryonic antigen-derived T cell epitope CAP-1-6D (CEA) into different positions of the hamster polyomavirus major capsid protein VP1. Independently from additional flanking linkers, yeast- expressed VP1 proteins harboring the CEA insertion between VP1 amino acid residues 80 and 89 (site 1) or 288 and 295 (site 4) or simultaneously at both positions assembled to chimeric virus-like particles (VLPs). BALB/c mice immunized with adjuvant-free VLPs developed VP1- and epitope-specific antibodies. The level of the CEA-specific antibody response was determined by the insertion site, the number of inserts, and the flanking linker. The strongest CEA-specific antibody response was observed in mice immunized with VP1 proteins harboring the CEA insert at site 1. Moreover, the CEA- specific antibodies in these mice were still detectable 6 mo after the final booster immunization. Our results indicate that hamster polyomavirus-derived VLPs represent a highly immunogenic carrier for foreign insertions that might be useful for clinical and therapeutic applications.
Y1  - 2007
UR  - http://www.liebertonline.com/vim
U6  - https://doi.org/10.1089/vim.2007.0023
SN  - 0882-8245
ER  -