TY  - JOUR
A1  - Forterre, Simone
A1  - Raila, Jens
A1  - Forterre, Franck
A1  - Brunnberg, Leo
A1  - Schweigert, Florian J.
T1  - Characterisation of transthyretin and retinol-binding protein in plasma and cerebrospinal fluid of dogs
N2  - The aim of this study was to investigate differences in concentrations of vitamin A, transthyretin (TTR) and retinol-binding protein (RBP) between plasma and cerebrospinal fluid (CSF) in dogs. RBP was detected using ELISA, and both RBP and TTR by Western blot analysis after separation on SDS-PAGE. Vitamin A was determined by high performance liquid chromatography. RBP and TTR as well as vitamin A were detected in all samples but at substantially lower concentrations in CSF compared to plasma. RBP in dog plasma showed a similar molecular mass to that of humans, whereas canine TTR had a lower molecular mass. Comparison between plasma and CSF showed that both RBP and TTR were of lower molecular mass in CSF. In CSF, RBP and retinol were present at 10-100-fold lower concentrations compared to plasma. Retinyl esters were present only in minute amounts in 5/17 samples. In conclusion, the CSF of dogs compared to humans is significantly different in terms of both quality and quantity of transport proteins for vitamin A.
Y1  - 2006
UR  - http://www.sciencedirect.com/science/journal/10900233
U6  - https://doi.org/10.1016/j.tvjl.2004.11.017
SN  - 1090-0233
ER  - 
TY  - JOUR
A1  - Forterre, Simone
A1  - Raila, Jens
A1  - Kohn, Barbara
A1  - Brunnberg, Leo
A1  - Schweigert, Florian J.
T1  - Protein profiling of organic stone matrix and urine from dogs with urolithiasis
N2  - Two-thirds of the organic matrix in urinary stones consists of proteins. Their relationship to calculogenesis remains controversial with regard to their effect as inhibitors or promoters during stone formation. The purpose of the present study was to determine the differences in peptide and protein pattern between the urine of stone formers (n = 23) and control dogs (n = 12), as well as between organic matrix of different urinary stones (struvite n = 11, calcium oxalate n = 8, uric acid n = 4) using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. Specific differences in protein and peptide profiles were found in the organic matrix of different mineral compositions. Characteristic differences were also found in urinary peptide and protein pattern especially in molecular masses below 20 kDa between affected and healthy dogs. Based on the obtained molecular masses they were in some cases tentatively identified as proteins that are known to be involved in stone formation in humans. The study shows that in dogs, specific-urinary peptides and proteins might be associated with urolithiasis. It indicates the importance to further characterize those proteins for possible diagnostic purposes in prognosis and therapy
Y1  - 2006
UR  - http://www3.interscience.wiley.com/journal/122630496/grouphome/home.html
U6  - https://doi.org/10.1111/j.1439-0396.2005.00590.x
SN  - 0931-2439
ER  -