TY  - JOUR
A1  - Srivastava, Abhishek
A1  - Murugaiyan, Jayaseelan
A1  - Garcia, Juan A. L.
A1  - De Corte, Daniele
A1  - Hoetzinger, Matthias
A1  - Eravci, Murat
A1  - Weise, Christoph
A1  - Kumar, Yadhu
A1  - Roesler, Uwe
A1  - Hahn, Martin W.
A1  - Grossart, Hans-Peter
T1  - Combined Methylome, Transcriptome and Proteome Analyses Document Rapid Acclimatization of a Bacterium to Environmental Changes
JF  - Frontiers in Microbiology
N2  - Polynucleobacter asymbioticus strain QLW-P1DMWA-1T represents a group of highly successful heterotrophic ultramicrobacteria that is frequently very abundant (up to 70% of total bacterioplankton) in freshwater habitats across all seven continents. This strain was originally isolated from a shallow Alpine pond characterized by rapid changes in water temperature and elevated UV radiation due to its location at an altitude of 1300 m. To elucidate the strain’s adjustment to fluctuating environmental conditions, we recorded changes occurring in its transcriptomic and proteomic profiles under contrasting experimental conditions by simulating thermal conditions in winter and summer as well as high UV irradiation. To analyze the potential connection between gene expression and regulation via methyl group modification of the genome, we also analyzed its methylome. The methylation pattern differed between the three treatments, pointing to its potential role in differential gene expression. An adaptive process due to evolutionary pressure in the genus was deduced by calculating the ratios of non-synonymous to synonymous substitution rates for 20 Polynucleobacter spp. genomes obtained from geographically diverse isolates. The results indicate purifying selection.
KW  - DNA modification
KW  - gene expression
KW  - freshwater heterotrophic bacteria
KW  - UV radiation
KW  - purifying selection
Y1  - 2020
U6  - https://doi.org/10.3389/fmicb.2020.544785
SN  - 1664-302X
VL  - 11
PB  - Frontiers Media
CY  - Lausanne
ER  - 
TY  - GEN
A1  - Srivastava, Abhishek
A1  - Murugaiyan, Jayaseelan
A1  - Garcia, Juan A. L.
A1  - De Corte, Daniele
A1  - Hoetzinger, Matthias
A1  - Eravci, Murat
A1  - Weise, Christoph
A1  - Kumar, Yadhu
A1  - Roesler, Uwe
A1  - Hahn, Martin W.
A1  - Grossart, Hans-Peter
T1  - Combined Methylome, Transcriptome and Proteome Analyses Document Rapid Acclimatization of a Bacterium to Environmental Changes
T2  - Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe
N2  - Polynucleobacter asymbioticus strain QLW-P1DMWA-1T represents a group of highly successful heterotrophic ultramicrobacteria that is frequently very abundant (up to 70% of total bacterioplankton) in freshwater habitats across all seven continents. This strain was originally isolated from a shallow Alpine pond characterized by rapid changes in water temperature and elevated UV radiation due to its location at an altitude of 1300 m. To elucidate the strain’s adjustment to fluctuating environmental conditions, we recorded changes occurring in its transcriptomic and proteomic profiles under contrasting experimental conditions by simulating thermal conditions in winter and summer as well as high UV irradiation. To analyze the potential connection between gene expression and regulation via methyl group modification of the genome, we also analyzed its methylome. The methylation pattern differed between the three treatments, pointing to its potential role in differential gene expression. An adaptive process due to evolutionary pressure in the genus was deduced by calculating the ratios of non-synonymous to synonymous substitution rates for 20 Polynucleobacter spp. genomes obtained from geographically diverse isolates. The results indicate purifying selection.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1011 
KW  - DNA modification
KW  - gene expression
KW  - freshwater heterotrophic bacteria
KW  - UV radiation
KW  - purifying selection
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-481993
SN  - 1866-8372
IS  - 1011
ER  -