TY  - GEN
A1  - Tzoneva, Rumiana
A1  - Stoyanova, Tihomira
A1  - Petrich, Annett
A1  - Popova, Desislava
A1  - Uzunova, Veselina
A1  - Albena, Momchilova
A1  - Chiantia, Salvatore
T1  - Effect of Erufosine on Membrane Lipid Order in Breast Cancer Cell Models
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Alkylphospholipids are a novel class of antineoplastic drugs showing remarkable therapeutic potential. Among them, erufosine (EPC3) is a promising drug for the treatment of several types of tumors. While EPC3 is supposed to exert its function by interacting with lipid membranes, the exact molecular mechanisms involved are not known yet. In this work, we applied a combination of several fluorescence microscopy and analytical chemistry approaches (i.e., scanning fluorescence correlation spectroscopy, line-scan fluorescence correlation spectroscopy, generalized polarization imaging, as well as thin layer and gas chromatography) to quantify the effect of EPC3 in biophysical models of the plasma membrane, as well as in cancer cell lines. Our results indicate that EPC3 affects lipid–lipid interactions in cellular membranes by decreasing lipid packing and increasing membrane disorder and fluidity. As a consequence of these alterations in the lateral organization of lipid bilayers, the diffusive dynamics of membrane proteins are also significantly increased. Taken together, these findings suggest that the mechanism of action of EPC3 could be linked to its effects on fundamental biophysical properties of lipid membranes, as well as on lipid metabolism in cancer cells.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1000 
KW  - alkylphospholipids
KW  - fluorescence microscopy
KW  - fluorescence correlation spectroscopy
KW  - lipids
KW  - plasma membrane
KW  - cancer
KW  - lipid–lipid interactions
KW  - membrane microdomains
KW  - membrane biophysics
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-477056
SN  - 1866-8372
IS  - 1000
ER  -