TY  - JOUR
A1  - Cisek, Richard
A1  - Tokarz, Danielle
A1  - Steup, Martin
A1  - Tetlow, Ian J.
A1  - Emes, Michael J.
A1  - Hebelstrup, Kim H.
A1  - Blennow, Andreas
A1  - Barzda, Virginijus
T1  - Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration
JF  - Biomedical optics express
N2  - Second harmonic generation (SHG) microscopy is employed to study changes in crystalline organization due to altered gene expression and hydration in barley starch granules. SHG intensity and susceptibility ratio values (R'(SHG)) are obtained using reduced Stokes-Mueller polarimetric microscopy. The maximum R'(SHG) values occur at moderate moisture indicating the narrowest orientation distribution of nonlinear dipoles from the cylindrical axis of glucan helices. The maximum SHG intensity occurs at the highest moisture and amylopectin content. These results support the hypothesis that SHG is caused by ordered hydrogen and hydroxyl bond networks which increase with hydration of starch granules. (C) 2015 Optical Society of America
Y1  - 2015
U6  - https://doi.org/10.1364/BOE.6.003694
SN  - 2156-7085
VL  - 6
IS  - 10
SP  - 3694
EP  - 3700
PB  - Optical Society of America
CY  - Washington
ER  -