TY  - JOUR
A1  - Sun, Zhiyong
A1  - Glebe, Ulrich
A1  - Charan, Himanshu
A1  - Böker, Alexander
A1  - Wu, Changzhu
T1  - Enzyme-Polymer Conjugates as Robust Pickering Interfacial Biocatalysts for Efficient Biotransformations and One-Pot Cascade Reactions
JF  - Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition
N2  - Despite the rapid development of Pickering interfacial catalysis (PIC) at liquid-liquid interfaces with chemocatalysts, the use of unstable biocatalysts at emulsion interfaces remains a technical challenge. Herein, we present a Pickering interfacial biocatalysis (PIB) platform based on robust and recyclable enzyme-polymer conjugates that act as both catalytic sites and stabilizers at the interface of Pickering emulsions. The conjugates were prepared by growing poly(N-isopropylacrylamide) on a fragile enzyme, benzaldehyde lyase, under physiological conditions. The mild in situ conjugation process preserved the enzyme structure, and the conjugates were used to emulsify a water-organic two-phase system into a stable Pickering emulsion, leading to a significantly larger interfacial area and a 270-fold improvement in catalytic performance as compared to the unemulsified two-phase system. The PIB system could be reused multiple times. Conjugates of other enzymes were also fabricated and applied for cascade reactions.
KW  - biphasic catalysis
KW  - cascade reactions
KW  - enzyme catalysis
KW  - enzyme-polymer conjugates
KW  - Pickering interfacial catalysis
Y1  - 2018
U6  - https://doi.org/10.1002/anie.201806049
SN  - 1433-7851
SN  - 1521-3773
VL  - 57
IS  - 42
SP  - 13810
EP  - 13814
PB  - Wiley-VCH
CY  - Weinheim
ER  -