TY  - JOUR
A1  - Hüttl, Christine
A1  - Hettrich, Cornelia
A1  - Riedel, Melanie
A1  - Henklein, Petra
A1  - Rawel, Harshadrai Manilal
A1  - Bier, Frank Fabian
T1  - Development of Peptidyl Lysine Dendrons: 1,3-Dipolar Cycloaddition for Peptide Coupling and Antibody Recognition
JF  - Chemical biology & drug design
N2  - A straightforward synthesis strategy to multimerize a peptide mimotopes for antibody B13-DE1 recognition is described based on lysine dendrons as multivalent scaffolds. Lysine dendrons that possess N-terminal alkyne residues at the periphery were quantitative functionalized with azido peptides using click chemistry. The solid-phase peptide synthesis (SPPS) allows preparing the peptide dendron in high purity and establishing the possibility of automation. The presented peptide dendron is a promising candidate as multivalent ligand and was used for antibody B13-DE1 recognition. The binding affinity increases with higher dendron generation without loss of specificity. The analysis of biospecific interaction between the synthesized peptide dendron and the antibody was done via surface plasmon resonance (SPR) technique. The presented results show a promising tool for investigations of antigen-antibody reactions.
KW  - click chemistry
KW  - lysine dendron
KW  - peptide mimotopes
KW  - solid-phase peptide synthesis
KW  - surface plasmon resonance
Y1  - 2015
U6  - https://doi.org/10.1111/cbdd.12444
SN  - 1747-0277
SN  - 1747-0285
VL  - 85
IS  - 5
SP  - 565
EP  - 573
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Couturier, Jean-Philippe
A1  - Sütterlin, Martin
A1  - Laschewsky, André
A1  - Hettrich, Cornelia
A1  - Wischerhoff, Erik
T1  - Responsive Inverse Opal Hydrogels for the Sensing of Macromolecules
JF  - Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition
N2  - Dual responsive inverse opal hydrogels were designed as autonomous sensor systems for (bio)macromolecules, exploiting the analyte-induced modulation of the opal's structural color. The systems that are based on oligo(ethylene glycol) macromonomers additionally incorporate comonomers with various recognition units. They combine a coil-to-globule collapse transition of the LCST type with sensitivity of the transition temperature toward molecular recognition processes. This enables the specific detection of macromolecular analytes, such as glycopolymers and proteins, by simple optical methods. While the inverse opal structure assists the effective diffusion even of large analytes into the photonic crystal, the stimulus responsiveness gives rise to strong shifts of the optical Bragg peak of more than 100nm upon analyte binding at a given temperature. The systems' design provides a versatile platform for the development of easy-to-use, fast, and low-cost sensors for pathogens.
KW  - hydrogels
KW  - photonic crystals
KW  - polymers
KW  - responsive materials
KW  - sensors
Y1  - 2015
U6  - https://doi.org/10.1002/anie.201500674
SN  - 1433-7851
SN  - 1521-3773
VL  - 54
IS  - 22
SP  - 6641
EP  - 6644
PB  - Wiley-VCH
CY  - Weinheim
ER  -